ReadiCleave™ iFluor® 514 Styramide

Power Styramide™ Signal Amplification (PSA) system is one of the most sensitive fluorescence imaging methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the commonly used tyramide (TSA) reagents. ReadiCleave™ Styramides are the new iteration of our PSA products that add the reversible capability to chemically remove the PSA staining on tissue or in cells if needed. As our other PSA reagents, the ReadiCleave™ Styramides are an excellent collection of multicolor reagents to simultaneously detect multiple targets in the same tissue samples. They provide an additional benefit, i.e., the PSA staining can be removed (if desired) while preserving the integrity of tissue samples. A specific protein is first recognized by its selective primary antibody. Subsequently, the target is stained by the HRP-secondary antibody conjugate of its immunoglobulin class. A ReadiCleave™ Styramide reagent (e.g., the bright green fluorescent ReadiCleave™ iFluor® 514 Styramide) is added subsequently. The HRP-antibody conjugate catalyzes the coupling reaction between ReadiCleave™ Styramides and the target protein in proximity. The PSA staining can be gently removed with nearly 100% efficiency using ReadiCleave™ AML Cleavage Buffer.