iFluor® 405 Styramide

Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA radicals have much higher reactivity than tyramide radicals, making the PSA system much faster, more robust and sensitive than the traditional TSA reagents. iFluor® 405 Styramide is a new unique blue fluorescent PSA reagent for multicolor application with our existing PSA and TSA reagents. It has the maximum fluorescence ~421 nm. iFluor® 405 Styramide is a superior replacement for Alexa Fluor 405 tyramide or other spectrally similar fluorescent tyramide conjugates or TSA reagents. AAT Bioquest offers the largest collection of TSA regents. We are the exclusive source of the superior PSA reagents for multicolor applications.

Fixed and permeabilized HeLa cells were incubated with rabbit anti-tubulin antibody, then labeled with HRP-labeled Goat anti-Rabbit IgG (Cat No. 16793), and detected using iFluor® 405 styramide (Cat No. 44908). Images were captured on a fluorescence microscope equipped with a DAPI filter set.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
44908	100 Slides	Price

References

View all 50 references: Citation Explorer

Immunofluorescent Staining of Adult Murine Paraffin-Embedded Skeletal Tissue.

Authors:

Felsenthal, Neta and Zelzer, Elazar

Journal:

Methods in molecular biology (Clifton, N.J.) (2021): 337-344

Highly Sensitive and Multiplexed In Situ RNA Profiling with Cleavable Fluorescent Tyramide.

Authors:

Xiao, Lu and Labaer, Joshua and Guo, Jia

Journal:

Cells (2021)

Single-cell RNA sequencing of human liver reveals hepatic stellate cell heterogeneity.

Authors:

Payen, Valéry L and Lavergne, Arnaud and Alevra Sarika, Niki and Colonval, Megan and Karim, Latifa and Deckers, Manon and Najimi, Mustapha and Coppieters, Wouter and Charloteaux, Benoît and Sokal, Etienne M and El Taghdouini, Adil

Journal:

JHEP reports : innovation in hepatology (2021): 100278

Multiplexed In Situ Protein Profiling with High-Performance Cleavable Fluorescent Tyramide.

Authors:

Pham, Thai and Liao, Renjie and Labaer, Joshua and Guo, Jia

Journal:

Molecules (Basel, Switzerland) (2021)

Accessibility-dependent topology studies of membrane proteins using a SpyTag/SpyCatcher protein-ligation system.

Authors:

Bae, Yoonji and Lee, Sang Kwon and Chae, Young Chan and Park, Chan Young and Kang, Sebyung

Journal:

International journal of biological macromolecules (2021): 171-178

Physical properties

Molecular weight	1174.49
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.48
Correction factor (280 nm)	0.77
Extinction coefficient (cm ^-1 M ^-1)	37000 ¹
Excitation (nm)	403
Emission (nm)	427
Quantum yield	0.91 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microscope
Excitation	DAPI/Violet filter set
Emission	DAPI/Violet filter set
Recommended plate	Black wall/clear bottom

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Page updated on October 13, 2025