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iFluor® 405 Styramide
Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA radicals have much higher reactivity than tyramide radicals, making the PSA system much faster, more robust and sensitive than the traditional TSA reagents. iFluor® 405 Styramide is a new unique blue fluorescent PSA reagent for multicolor application with our existing PSA and TSA reagents. It has the maximum fluorescence ~421 nm. iFluor® 405 Styramide is a superior replacement for Alexa Fluor 405 tyramide or other spectrally similar fluorescent tyramide conjugates or TSA reagents. AAT Bioquest offers the largest collection of TSA regents. We are the exclusive source of the superior PSA reagents for multicolor applications.
Fixed and permeabilized HeLa cells were incubated with rabbit anti-tubulin antibody, then labeled with HRP-labeled Goat anti-Rabbit IgG (Cat No. 16793), and detected using iFluor® 405 styramide (Cat No. 44908). Images were captured on a fluorescence microscope equipped with a DAPI filter set.
Fixed and permeabilized HeLa cells were incubated with rabbit anti-tubulin antibody, then labeled with HRP-labeled Goat anti-Rabbit IgG (Cat No. 16793), and detected using iFluor® 405 styramide (Cat No. 44908). Images were captured on a fluorescence microscope equipped with a DAPI filter set.
CatalogSize
Price
Quantity
44908100 Slides
Price
 
Physical properties

Molecular weight1174.49
SolventDMSO
Spectral properties

Correction factor (260 nm)0.48
Correction factor (280 nm)0.77
Extinction coefficient (cm -1 M -1)
37000
1
Excitation (nm)403
Emission (nm)427
Quantum yield
0.91
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Instrument settings

Fluorescence microscope
ExcitationDAPI/Violet filter set
EmissionDAPI/Violet filter set
Recommended plateBlack wall/clear bottom
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Page updated on October 13, 2025