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ReadiLink™ Rapid mFluor™ Violet 420 Antibody Labeling Kit
Microscale Optimized for Labeling 50 μg Antibody Per Reaction
AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications. These dyes can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm). mFluor™ Violet 420 dyes have fluorescence excitation and emission maxima of ~405 nm and ~420 nm respectively. These spectral characteristics make them an excellent replacement for Cascade® Blue labeling dye ( Cascade® Blue is the trademark of InvitroGen). mFluor™ Violet 420 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. This kit has all the essential components for labeling ~100 µg antibody with no column purification needed. mFluor™ Violet 420 SE antibody labeling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the Violet Laser-excitable mFluor™ Violet 420 SE.

 

readilinkworkflow

 

Figure 1. Overview of the ReadiLink™ Rapid Antibody Labeling protocol. In just two simple steps, and with no purification necessary, covalently label microgram amounts of antibodies in under an hour.

Detection of CD4 expression on human peripheral blood lymphocytes stained by flow cytometry. Human PBMCs were stained with mFluor™ Violet 420 anti-human CD4 monoclonal antibody *SK3*. The fluorescence signal was monitored using an ACEA NovoCyte flow cytometer in the Pacific Blue channel.
Detection of CD4 expression on human peripheral blood lymphocytes stained by flow cytometry. Human PBMCs were stained with mFluor™ Violet 420 anti-human CD4 monoclonal antibody *SK3*. The fluorescence signal was monitored using an ACEA NovoCyte flow cytometer in the Pacific Blue channel.
CatalogSize
Price
Quantity
11052 Labelings
Price
 
Spectral properties

Extinction coefficient (cm -1 M -1)
37000
1
Excitation (nm)403
Emission (nm)427
Quantum yield
0.91
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
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Page updated on October 19, 2025