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ReadiLink™ Rapid mFluor™ Violet 510 Antibody Labeling Kit
Microscale Optimized for Labeling 50 μg Antibody Per Reaction
AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm). mFluor™ Violet 510 dyes have fluorescence excitation and emission maxima of ~405 nm and ~510 nm respectively. mFluor™ Violet 510 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. This kit has all the essential components to perform two separate labeling reactions with no column purification needed. Each of the two vials of mFluor™ Violet 510 SE provided in the kit is optimized for labeling ~50 µg antibody. mFluor™ Violet 510 SE antibody labeling kit provides a convenient method to label small amounts of monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the Violet Laser-excitable mFluor™ Violet 510 SE.

 

readilinkworkflow

 

Figure 1. Overview of the ReadiLink™ Rapid Antibody Labeling protocol. In just two simple steps, and with no purification necessary, covalently label microgram amounts of antibodies in under an hour.

Flow cytometric analysis of cells undergoing apoptosis using Annexin V-mFluor™ Violet 510. Jurkat cells were treated with (red) or without 1 µM staurosporine (green) for 4 hours at 37 ºC. Cells were then incubated with Annexin V labeled using the ReadiLink™ Rapid mFluor™ Violet 510 Antibody Labeling Kit (Cat No. 1110) for 30 minutes to identify apoptotic cells. Fluorescence intensity was measured using an ACEA NovoCyte flow cytometer.
Flow cytometric analysis of cells undergoing apoptosis using Annexin V-mFluor™ Violet 510. Jurkat cells were treated with (red) or without 1 µM staurosporine (green) for 4 hours at 37 ºC. Cells were then incubated with Annexin V labeled using the ReadiLink™ Rapid mFluor™ Violet 510 Antibody Labeling Kit (Cat No. 1110) for 30 minutes to identify apoptotic cells. Fluorescence intensity was measured using an ACEA NovoCyte flow cytometer.
CatalogSize
Price
Quantity
11102 Labelings
Price
 
Spectral properties

Absorbance (nm)410
Correction factor (260 nm)0.464
Correction factor (280 nm)0.366
Extinction coefficient (cm -1 M -1)
25000
1
Excitation (nm)412
Emission (nm)505
Quantum yield
0.86
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
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Page updated on October 29, 2025