ReadiUse™ MTS Reagent *50 mM aqueous solution*
Ordering information
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Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 487.51 |
Solvent | Water |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Refrigerated (2-8 °C); Minimize light exposure |
Related products
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See also: Cell Proliferation Assays, Cell Viability Assays
CAS 138169-43-4 | Molecular weight 487.51 |
MTS reagent is one of the most common water-soluble tetrazolium reagents that are widely used to quantify cell viability by absorption spectrophotometry. A variety of tetrazolium inner salts are used for determining cell viability in proliferation assays by monitoring their color changes. Metabolically active cells reduce colorless tetrazolium inner salts into the colored formazan products that might be either soluble or insoluble in cell culture media depending on the structures of tetrazolium reagents. The conversion of tetrazolium inner salts to formazans is thought to be carried out by NAD(P)H-dependent dehydrogenase enzymes.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of ReadiUse™ MTS Reagent *50 mM aqueous solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 205.124 µL | 1.026 mL | 2.051 mL | 10.256 mL | 20.512 mL |
5 mM | 41.025 µL | 205.124 µL | 410.248 µL | 2.051 mL | 4.102 mL |
10 mM | 20.512 µL | 102.562 µL | 205.124 µL | 1.026 mL | 2.051 mL |
Molarity calculator
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References
View all 39 references: Citation Explorer
The edge effect: A global problem. The trouble with culturing cells in 96-well plates.
Authors: Mansoury, Morva and Hamed, Maya and Karmustaji, Rashid and Al Hannan, Fatima and Safrany, Stephen T
Journal: Biochemistry and biophysics reports (2021): 100987
Authors: Mansoury, Morva and Hamed, Maya and Karmustaji, Rashid and Al Hannan, Fatima and Safrany, Stephen T
Journal: Biochemistry and biophysics reports (2021): 100987
Profiling the Neoplasm Microenvironment of Silica Nanomaterial-Derived Scaffolds of Single, 2-, and 3-Composite Systems.
Authors: Akpe, Victor and Murhekar, Shweta and Kim, Tak H and Brown, Christopher L and Cock, Ian E
Journal: Assay and drug development technologies (2021): 191-203
Authors: Akpe, Victor and Murhekar, Shweta and Kim, Tak H and Brown, Christopher L and Cock, Ian E
Journal: Assay and drug development technologies (2021): 191-203
A Modified MTS Proliferation Assay for Suspended Cells to Avoid the Interference by Hydralazine and β-Mercaptoethanol.
Authors: Wang, Yutang and Nguyen, Dinh Tam and Yang, Guang and Anesi, Jack and Kelly, Jason and Chai, Zhonglin and Ahmady, Fahima and Charchar, Fadi and Golledge, Jonathan
Journal: Assay and drug development technologies (2021): 184-190
Authors: Wang, Yutang and Nguyen, Dinh Tam and Yang, Guang and Anesi, Jack and Kelly, Jason and Chai, Zhonglin and Ahmady, Fahima and Charchar, Fadi and Golledge, Jonathan
Journal: Assay and drug development technologies (2021): 184-190
Synthesis, structural characterization, and prospects for new cobalt (II) complexes with thiocarbamoyl-pyrazoline ligands as promising antifungal agents.
Authors: Dias, Bianca Boni and da Silva Dantas, Fabiana Gomes and Galvão, Fernanda and Cupozak-Pinheiro, Wellinton Jhon and Wender, Heberton and Pizzuti, Lucas and Rosa, Persiely Pires and Tenório, Kátia Veronica and Gatto, Claudia Cristina and Negri, Melyssa and Casagrande, Gleison Antônio and de Oliveira, Kelly Mari Pires
Journal: Journal of inorganic biochemistry (2020): 111277
Authors: Dias, Bianca Boni and da Silva Dantas, Fabiana Gomes and Galvão, Fernanda and Cupozak-Pinheiro, Wellinton Jhon and Wender, Heberton and Pizzuti, Lucas and Rosa, Persiely Pires and Tenório, Kátia Veronica and Gatto, Claudia Cristina and Negri, Melyssa and Casagrande, Gleison Antônio and de Oliveira, Kelly Mari Pires
Journal: Journal of inorganic biochemistry (2020): 111277
The Predictive Value of Estrogen Receptor 1 on Adjuvant Chemotherapy in Locally Advanced Colorectal Cancer: A Retrospective Analysis With Independent Validation and Its Potential Mechanism.
Authors: Ye, Shu-Biao and Cheng, Yi-Kan and Deng, Ru and Deng, Yanhong and Li, Peisi and Zhang, Lin and Lan, Ping
Journal: Frontiers in oncology (2020): 214
Authors: Ye, Shu-Biao and Cheng, Yi-Kan and Deng, Ru and Deng, Yanhong and Li, Peisi and Zhang, Lin and Lan, Ping
Journal: Frontiers in oncology (2020): 214
Calcineurin/NFAT Signaling Modulates Pulmonary Artery Smooth Muscle Cell Proliferation, Migration and Apoptosis in Monocrotaline-Induced Pulmonary Arterial Hypertension Rats.
Authors: He, Rui-Lan and Wu, Zhi-Juan and Liu, Xiao-Ru and Gui, Long-Xin and Wang, Rui-Xing and Lin, Mo-Jun
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2018): 172-189
Authors: He, Rui-Lan and Wu, Zhi-Juan and Liu, Xiao-Ru and Gui, Long-Xin and Wang, Rui-Xing and Lin, Mo-Jun
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2018): 172-189
Glutathione release through connexin hemichannels: Implications for chemical modification of pores permeable to large molecules.
Authors: Tong, Xuhui and Lopez, William and Ramachandran, Jayalakshmi and Ayad, Wafaa A and Liu, Yu and Lopez-Rodriguez, Angelica and Harris, Andrew L and Contreras, Jorge E
Journal: The Journal of general physiology (2015): 245-54
Authors: Tong, Xuhui and Lopez, William and Ramachandran, Jayalakshmi and Ayad, Wafaa A and Liu, Yu and Lopez-Rodriguez, Angelica and Harris, Andrew L and Contreras, Jorge E
Journal: The Journal of general physiology (2015): 245-54
Caution is required in interpretation of mutations in the voltage sensing domain of voltage gated channels as evidence for gating mechanisms.
Authors: Kariev, Alisher M and Green, Michael E
Journal: International journal of molecular sciences (2015): 1627-43
Authors: Kariev, Alisher M and Green, Michael E
Journal: International journal of molecular sciences (2015): 1627-43
Cysteine accessibility probes timing and extent of NBD separation along the dimer interface in gating CFTR channels.
Authors: Chaves, Luiz A Poletto and Gadsby, David C
Journal: The Journal of general physiology (2015): 261-83
Authors: Chaves, Luiz A Poletto and Gadsby, David C
Journal: The Journal of general physiology (2015): 261-83
CHARMM-GUI PDB manipulator for advanced modeling and simulations of proteins containing nonstandard residues.
Authors: Jo, Sunhwan and Cheng, Xi and Islam, Shahidul M and Huang, Lei and Rui, Huan and Zhu, Allen and Lee, Hui Sun and Qi, Yifei and Han, Wei and Vanommeslaeghe, Kenno and MacKerell, Alexander D and Roux, Benoît and Im, Wonpil
Journal: Advances in protein chemistry and structural biology (2014): 235-65
Authors: Jo, Sunhwan and Cheng, Xi and Islam, Shahidul M and Huang, Lei and Rui, Huan and Zhu, Allen and Lee, Hui Sun and Qi, Yifei and Han, Wei and Vanommeslaeghe, Kenno and MacKerell, Alexander D and Roux, Benoît and Im, Wonpil
Journal: Advances in protein chemistry and structural biology (2014): 235-65
Application notes
Multicolor Analysis of Cell Proliferation Using a New Panel of CytoTell™ Fluorescent Dyes
Relative Brightness of Fluorescent Dyes
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Relative Brightness of Fluorescent Dyes
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
FAQ
AssayWise
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Unlocking the Potential of 3D Cell Culture: A Guide to Assay Optimization
Nucleic Acid Detection, Quantification and Imaging
A practical guide for use of PE and APC in flow cytometry
Calbryte™ series now available