Do you have any cytotoxicity information for your Nuclear LCS dyes?

Q: Do you have any cytotoxicity information for your Nuclear LCS dyes?

We’ve tested our Nuclear LCS dyes under these conditions: HeLa cells were labeled with LCS dyes at various concentrations, from 5 uM - 0.5 uM, for 30 mins in Henderson-Hasselbalch (HH) buffer. Cells were then washed with HH buffer 2-3 times. After washing, cells were incubated for 24 hours in a regular DMEM medium. Fluorescence images were taken in cells before and after incubation in DMEM to test for long-term stability. Cell Meter™ Colorimetric WST-8 Cell Quantification Kit (Cat#22770) was applied for cytotoxicity testing. Cytotoxicity of LCS dyes: Nuclear Violet™ LCS1 (Cat#17543) has the least cytotoxic effect. Cells labeled with 5 uM of Nuclear Red™ LCS1 (Cat#17542) were still viable. Cells labeled with Nuclear Green™ LCS1 (Cat#17540) or Nuclear Red™ LCS1 at concentrations lower than 1.5 uM remained viable after 24 hours. Nuclear Red™ LCS2 (Cat#17545) showed similar cytotoxic effects compared to Nuclear Green™ LCS1, BUT the fluorescence signal was low. All of our Nuclear LCS1/LCS2 dyes are quite stable. In general, photostability can be ranked as such: Red (more stable) > Green > Violet and Blue (least stable)

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Accepted Answer

We&rsquo;ve tested our Nuclear LCS dyes under these conditions:
HeLa cells were labeled with LCS dyes at various concentrations, from 5 uM - 0.5 uM, for 30 mins in Henderson-Hasselbalch (HH) buffer. Cells were then washed with HH buffer 2-3 times. After washing, cells were incubated for 24 hours in a regular DMEM medium. Fluorescence images were taken in cells before and after incubation in DMEM to test for long-term stability. Cell Meter&trade; Colorimetric WST-8 Cell Quantification Kit (Cat#22770) was applied for cytotoxicity testing.

Cytotoxicity of LCS dyes:

Nuclear Violet&trade; LCS1 (Cat#17543) has the least cytotoxic effect.
Cells labeled with 5 uM of Nuclear Red&trade; LCS1 (Cat#17542) were still viable.   
Cells labeled with Nuclear Green&trade; LCS1 (Cat#17540) or Nuclear Red&trade; LCS1 at concentrations lower than 1.5 uM remained viable after 24 hours. 
Nuclear Red&trade; LCS2 (Cat#17545) showed similar cytotoxic effects compared to Nuclear Green&trade; LCS1, BUT the fluorescence signal was low.

All of our Nuclear LCS1/LCS2 dyes are quite stable.

In general, photostability can be ranked as such:

Red (more stable) > Green > Violet and Blue (least stable)

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