Do you have any cytotoxicity information for your Nuclear LCS dyes?
Posted June 12, 2019
I am interested in using your nucleic acid stains for an application I am designing. Do you have any information on the cytotoxicity, long-term stability and photostability of your Nuclear LCS1/LCS2 dyes?
We’ve tested our Nuclear LCS dyes under these conditions:
HeLa cells were labeled with LCS dyes at various concentrations, from 5 uM - 0.5 uM, for 30 mins in Henderson-Hasselbalch (HH) buffer. Cells were then washed with HH buffer 2-3 times. After washing, cells were incubated for 24 hours in a regular DMEM medium. Fluorescence images were taken in cells before and after incubation in DMEM to test for long-term stability. Cell Meter™ Colorimetric WST-8 Cell Quantification Kit (Cat#22770) was applied for cytotoxicity testing.
- Cytotoxicity of LCS dyes:
- Nuclear Violet™ LCS1 (Cat#17543) has the least cytotoxic effect.
- Cells labeled with 5 uM of Nuclear Red™ LCS1 (Cat#17542) were still viable.
- Cells labeled with Nuclear Green™ LCS1 (Cat#17540) or Nuclear Red™ LCS1 at concentrations lower than 1.5 uM remained viable after 24 hours.
- Nuclear Red™ LCS2 (Cat#17545) showed similar cytotoxic effects compared to Nuclear Green™ LCS1, BUT the fluorescence signal was low.
- All of our Nuclear LCS1/LCS2 dyes are quite stable.
- In general, photostability can be ranked as such:
- Red (more stable) > Green > Violet and Blue (least stable)