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Relative Brightness of Fluorescent Dyes

DNA Dyes



Fluorescence images of oleic acid treated HeLa cells stained with Cell Navigator® Lipid Droplets Fluorescence Assay Kit (Cat#22735) and Nuclear Green™ LCS1 (Cat#17540).

 

Table 1. Relative Brightness of DNA Dyes

Dye Name
Function
Laser
Filter
Brightness
DAPIDNA355 nmDAPI6
Nuclear Blue™ DCS1DNA355 nmDAPI6
Propidium iodideDNA488 nmTexas Red2
Nuclear Green™DNA488 nmFITC6
Propidium iodideDNA532 nmTexas Red3
Nuclear Red™DNA633 nmCy5.56

 

Proliferation Dyes



Cell proliferation assay with CytoTell™ Green and CFSE. Jurkat cells (~2×106 cells/mL) were stained with CytoTell™ Green or CFSE on Day 0. The cells were passed serially at 1:1 ratio on the day specified. Fluorescence intensity was measured with ACEA NovoCyte 3000 flow cytometer in FITC channel on the day of passage. Successive generations were represented by different colors.

 

Table 2. Relative Brightness of Proliferation Dyes

Dye Name
Function
Laser
Filter
Brightness
CytoTell™ BlueProliferation405 nmDAPI6
CFSEProliferation488 nmFITC7
CytoTell™ GreenProliferation488 nmFITC7
CytoTell™ OrangeProliferation488 nmCy3/TRITC7
CytoTell™ UltraGreenProliferation488 nmFITC7
CytoTell™ Red 590Proliferation561 nmCy37
CytoTell™ Red 650Proliferation633 nmCy57

 

Viability Dyes



Images of Live HeLa cells stained with CytoCalcein Violet 500 (Cat.22013). Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17535).

 

Table 3. Relative Brightness of Viability Dyes

Dye Name
Function
Laser
Filter
Brightness
Calcein BlueViability405 nmDAPI6
Calcein UltraBlue™ AMViability405 nmDAPI6
CytoCalcein™ Violet 450Viability405 nmDAPI7
CytoCalcein™ Violet 500Viability405 nmFITC7
7-AADViability488 nmCy51
Calcein *UltraPure Grade*Viability488 nmCy51
Calcein Orange™Viability488 nmTRITC7
Calcein UltraGreen™ AMViability488 nmFITC7
7-AADViability532 nmCy52
Calcein Red™ AMViability561 nmCy3/TRITC7

 

Labeling Dyes



HeLa cells were incubated with (Tubulin+) or without (Tubulin-) mouse anti-tubulin followed by iFluor® 488 goat anti-mouse IgG conjugate (Green, Left) or Alexa Fluor® 488 goat anti-mouse IgG conjugate (Green, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue).

 

Table 4. Relative Brightness of Labeling Dyes

Dye Name
Function
Laser
Filter
Brightness
Alexa Fluor 350Labeling355 nmDAPI2
iFluor® 350Labeling355 nmDAPI6
iFluor® 450Labeling405 nmFITC5
Pacific GreenLabeling405 nmFITC5
Pacific OrangeLabeling405 nmTRITC2
iFluor® 405Labeling405 nmDAPI6
iFluor® 430 Labeling405 nmFITC6
PacBlueLabeling405 nmDAPI2
Alexa Fluor 555Labeling488 nmTRITC4
Cy3Labeling488 nmTRITC4


Original created on July 19, 2019, last updated on July 19, 2019
Tagged under: iFluor dyes, AAT, APC, Alexa Fluor 488, Allophycocyanin, CytoTell, DNA, DAPI, Propidium iodide (PI), flow cytometry, flow cytometer, Flow Cytometry