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Relative Brightness of Fluorescent Dyes

DNA Dyes


Figure 1. Fluorescence images of oleic acid treated HeLa cells stained with Cell Navigator® Lipid Droplets Fluorescence Assay Kit (Cat#22735) and Nuclear Green™ LCS1 (Cat#17540).

Dye Name Function  Laser Filter Brightness
DAPI DNA 355 nm DAPI 6
Nuclear Blue DNA 355 nm DAPI 6
Propidium Iodide DNA 488 nm Texas Red 2
Nuclear Green DNA 488 nm FITC 6
Nuclear Orange DNA 488 nm Cy3 6
Propidium Iodide DNA 532 nm Texas Red 3
Nuclear Red DNA 633 nm Cy5.5 6

 

Proliferation Dyes


Figure 2. Cell proliferation assay with CytoTell™ Green and CFSE. Jurkat cells (~2×106 cells/mL) were stained with CytoTell™ Green or CFSE on Day 0. The cells were passed serially at 1:1 ratio on the day specified. Fluorescence intensity was measured with ACEA NovoCyte 3000 flow cytometer in FITC channel on the day of passage. Successive generations were represented by different colors.

Dye Name Function Laser Filter Brightness
CytoTell Blue Proliferation 405 nm DAPI 6
CFSE Proliferation 488 nm FITC 7
CytoTell Green Proliferation 488 nm FITC 7
CytoTell Orange Proliferation 488 nm Cy3/TRITC 7
CytoTell UltraGreen Proliferation 488 nm FITC 7
CytoTell Red 590 Proliferation 561 nm Cy3 7
CytoTell Red 650 Proliferation 633 nm Cy5 7

 

Viability Dyes


Figure 3. Images of Live HeLa cells stained with CytoCalcein Violet 500 (Cat.22013). Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17535).

Dye Name Function Laser Filter Brightness
Calcein Blue Viability 405 nm DAPI 6
Calcein UltraBlue Viability 405 nm DAPI 6
CytoCalcein Violet 450 Viability 405 nm DAPI 7
CytoCalcein Violet 500 Viability 405 nm FITC 7
7-AAD Viability 488 nm Cy5 1
Calcein Viability 488 nm FITC 7
Calcein Orange Viability 488 nm TRITC 7
Calcein UltraGreen Viability 488 nm FITC 7
7-AAD Viability 532 nm Cy5 2
Calcein Red Viability 561nm Cy3/TRITC 7
Calcein Deep Red Viability 633 nm Cy5 7

 

Labeling Dyes 


Figure 4. HeLa cells were incubated with (Tubulin+) or without (Tubulin-) mouse anti-tubulin followed by iFluor® 488 goat anti-mouse IgG conjugate (Green, Left) or Alexa Fluor® 488 goat anti-mouse IgG conjugate (Green, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue).

Dye Name Function Laser Filter Brightness
Alexa Fluor 350 Labeling 355 nm DAPI 2
iFluor 350 Labeling 355 nm DAPI 6
iFluor 450 Labeling 405 nm FITC 5
Pacific Green Labeling 405 nm FITC 5
Pacific Orange Labeling 405 nm TRITC 2
iFluor 405 Labeling 405 nm DAPI 6
iFluor 430 Labeling 405 nm FITC 6
Pacific Blue Labeling 405 nm DAPI 2
Alexa Fluor 555 Labeling 488 nm TRITC 4
Cy3 Labeling 488 nm TRITC 4
Alexa Fluor 488 Labeling 488 nm FITC 5
FITC Labeling 488 nm FITC 7
iFluor 488 Labeling 488 nm FITC 7
Oregon Green Labeling 488 nm FITC 7
TRITC Labeling 488 nm TRITC 7
PE-Alexa Fluor 594 Labeling 488 nm Texas Red 9
PE-Alexa Fluor 750 Labeling 488 nm Cy7 9
PE-iFluor 594 Labeling 488 nm Texas Red 9
PE-iFluor 750 Labeling 488 nm Cy7 9
PE Labeling 488 nm TRITC 10
PE-Alexa Fluor 647 Labeling 488 nm Cy5 7
PE-Cy5 Labeling 488 nm Cy5.5 10
PE-Cy5.5 Labeling 488 nm Cy5.5 7
PE-Cy7 Labeling 488 nm Cy7 10
PE-iFluor 647 Labeling 488 nm Cy5 10
PE-Texas Red Labeling 488 nm Texas Red 10
Alexa Fluor 555 Labeling 532 nm TRITC 5
Cy3 Labeling 532 nm TRITC 5
Alexa Fluor 532 Labeling 532 nm TRITC 7
iFluor 514 Labeling 532 nm Cy3/TRITC (590/40) 7
iFluor 532 Labeling 532 nm Cy3/TRITC (590/40) 7
iFluor 546 Labeling 532 nm Cy3/TRITC (590/40) 7
Alexa Fluor 594 Labeling 561 nm Texas Red 6
iFluor 610 Labeling 561 nm Texas Red (610/40) 6
Texas Red Labeling 561 nm Texas Red 6
iFluor 555 Labeling 561 nm Cy3/TRITC (590/40) 7
iFluor 568 Labeling 561 nm Cy3/TRITC (590/40) 7
iFluor 594 Labeling 561 nm Texas Red (610/40) 7
Alexa Fluor 750 Labeling 633 nm Cy7 4
iFluor 750 Labeling 633 nm Cy7 (780/60) 4
iFluor 680 Labeling 633 nm Custom (712/25) 5
Alexa Fluor 680 Labeling 633 nm Cy5.5 6
Alexa Fluor 700 Labeling 633 nm Cy7 6
Cy7 Labeling 633 nm Cy7 6
iFluor 647 Labeling 633 nm Cy5 (660/40) 6
iFluor 660 Labeling 633 nm Cy5 (660/40) 6
iFluor 700 Labeling 633 nm Custom (712/25) 6
Alexa Fluor 647 Labeling 633 nm Cy5 7
Cy5 Labeling 633 nm Cy5 7
iFluor 633 Labeling 633 nm Cy5 (660/40) 7
APC-Alexa Fluor 750 Labeling 633 nm Cy7 8
APC-Cy5.5 Labeling 633 nm Cy5.5 8
APC-Cy7 Labeling 633 nm Cy7 5
APC-iFluor 700 Labeling 633 nm Cy7 8
APC-iFluor 750 Labeling 633 nm Cy7 8
APC Labeling 633 nm Cy5 9
iFluor 790 Labeling 633 nm Cy7 (780/60) 3
iFluor 800 Labeling 633 nm Custom (840/20) 3
iFluor 810 Labeling 633 nm Custom (840/20) 3
iFluor 820 Labeling 633 nm Custom (840/20) 3
iFluor 860 Labeling 633 nm Custom (885/40) 3

 



Original created on July 19, 2019, last updated on July 19, 2019
Tagged under: iFluor dyes, AAT, APC, Alexa Fluor 488, Allophycocyanin, CytoTell, DNA, DAPI, Propidium iodide (PI), flow cytometry, flow cytometer, Flow Cytometry