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AAT Bioquest

XFD680 NHS Ester *Same Structure to Alexa Fluor™ 680 NHS Ester*

Flow cytometry analysis of PBMC stained with XFD680 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the XFD680 specific R4-A channel. XFD680 is the same structure as Alexa Fluor® 680.
Flow cytometry analysis of PBMC stained with XFD680 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the XFD680 specific R4-A channel. XFD680 is the same structure as Alexa Fluor® 680.
Flow cytometry analysis of PBMC stained with XFD680 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the XFD680 specific R4-A channel. XFD680 is the same structure as Alexa Fluor® 680.
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Physical properties
Molecular weight~1200
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.00
Correction Factor (280 nm)0.05
Extinction coefficient (cm -1 M -1)184000
Excitation (nm)681
Emission (nm)704
Quantum yield0.361
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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XFD532 C5 Maleimide *Same Structure to Alexa Fluor™ 532 C5 Maleimide*
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XFD488 Hydroxylamine *Same Structure to Alexa Fluor™ 488 Hydroxylamine*
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XFD350 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350*
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XFD488 amine *Same Structure to Alexa Fluor™ 488 amine*
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine*
XFD350-streptavidin conjugate *XFD350 Same Structure to Alexa Fluor™ 350*
XFD488-streptavidin conjugate *XFD488 Same Structure to Alexa Fluor™ 488*
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XFD350 Phalloidin *XFD350 Same Structure to Alexa Fluor™ 350*
XFD488 Phalloidin *XFD488 Same Structure to Alexa Fluor™ 488*
XFD594 Phalloidin *XFD594 Same Structure to Alexa Fluor™ 594*
XFD532 acid *Same Structure to Alexa Fluor™ 532 acid*
XFD488 acid *Same Structure to Alexa Fluor™ 488 acid*
XFD488 tyramide reagent *Same Structure to Alexa Fluor™ 488 tyramide*
XFD546 tyramide reagent *Same Structure to Alexa Fluor™ 546 tyramide*
XFD594 tyramide reagent *Same Structure to Alexa Fluor™ 594 tyramide*
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XFD647 C2 Maleimide *Same Structure to Alexa Fluor™ 647 C2 Maleimide*
XFD350 tyramide reagent *Same Structure to Alexa Fluor™ 350 tyramide*
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XFD350 acid *Same Structure to Alexa Fluor™ 350 acid*
XFD546 acid *Same Structure to Alexa Fluor™ 546 acid*
XFD568 acid *Same Structure to Alexa Fluor™ 568 acid*
XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine*
XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde*
XFD™488-dUTP *1 mM in Tris Buffer (pH 7.5)*
XFD514 acid
XFD514 tyramide
XFD532 tyramide
XFD647 Azide
XFD647 Alkyne
XFD488 TCO
XFD532 amine
XFD555 amine
XFD568 amine
XFD647 amine
XFD750 amine
XFD555 acid
XFD647 acid
XFD750 acid
XFD700 acid
XFD647 Phalloidin *equivalent to Alexa Fluor® 647 phalloidin*
Show More (42)

OverviewpdfSDSpdfProtocol


Molecular weight
~1200
Correction Factor (260 nm)
0.00
Correction Factor (280 nm)
0.05
Extinction coefficient (cm -1 M -1)
184000
Excitation (nm)
681
Emission (nm)
704
Quantum yield
0.361
XFD680 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 680 (Alexa Fluor® is the trademark of ThermoFisher). It is a bright red fluorescent dye. XFD680 dye is water soluble and pH-insensitive from pH 4 to pH 10. The NHS ester (or succinimidyl ester) of XFD680 is the most convenient amine-reactive form for conjugating this dye to a protein or antibody.

Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.00
Correction Factor (280 nm)0.05
Extinction coefficient (cm -1 M -1)184000
Excitation (nm)681
Emission (nm)704
Quantum yield0.361

Images


Citations


View all 6 citations: Citation Explorer
A combined solvatochromic shift and TDDFT study probing solute-solvent interactions of blue fluorescent Alexa Fluor 350 dye: Evaluation of ground and excited state dipole moments
Authors: Patil, M. K., Kotresh, M. G., Inamdar, S. R.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2019): 142-152
Photobleaching Comparison of R-Phycoerythrin-Streptavidin and Streptavidin-Alexa Fluor 568 in a Breast Cancer Cell Line
Authors: Ostad, S. N., Babaei, S., Bayat, A. A., Mahmoudian, J.
Journal: Monoclon Antib Immunodiagn Immunother (2019): 25-29
Comparison between photostability of Alexa Fluor 448 and Alexa Fluor 647 with conventional dyes FITC and APC by flow cytometry
Authors: Rai, S., Bhardwaj, U., Misra, A., Singh, S., Gupta, R.
Journal: Int J Lab Hematol (2018): e52-e54
Development of new hCaM-Alexa Fluor((R)) biosensors for a wide range of ligands
Authors: Velazquez-Lopez, I., Leon-Cruz, E., Pardo, J. P., Sosa-Peinado, A., Gonzalez-Andrade, M.
Journal: Anal Biochem (2017): 13-22
Neuroanatomical basis of clinical joint application of &quot;Jinggu&quot; (BL 64, a source-acupoint) and &quot;Dazhong&quot; (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
Authors: Cui, J. J., Zhu, X. L., Ji, C. F., Jing, X. H., Bai, W. Z.
Journal: Zhen Ci Yan Jiu (2011): 262-7
Neuroanatomical characteristics of acupoint &quot;Chengshan&quot; (BL 57) in the rat: a cholera toxin subunit B conjugated with Alexa Fluor 488 method study
Authors: Zhu, X. L., Bai, W. Z., Wu, F. D., Jiang, J., Jing, X. H.
Journal: Zhen Ci Yan Jiu (2010): 433-7

References


View all 39 references: Citation Explorer
Development of new hCaM-Alexa Fluor(R) biosensors for a wide range of ligands
Authors: Velazquez-Lopez, I.; Leon-Cruz, E.; Pardo, J. P.; Sosa-Peinado, A.; Gonzalez-Andrade, M.
Journal: Anal Biochem (2017): 13-22
Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye
Authors: Winne, J. M.; Irani, N. G.; Van den Begin, J.; Madder, A.
Journal: Methods Mol Biol (2017): 21-Sep
Alteration of AMPA Receptor-Mediated Synaptic Transmission by Alexa Fluor 488 and 594 in Cerebellar Stellate Cells
Authors: Maroteaux, M.; Liu, S. J.
Journal: eNeuro (2016)
Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments
Authors: Grate, J. W.; Mo, K. F.; Shin, Y.; Vasdekis, A.; Warner, M. G.; Kelly, R. T.; Orr, G.; Hu, D.; Dehoff, K. J.; Brockman, F. J.; Wilkins, M. J.
Journal: Bioconjug Chem (2015): 593-601
In vivo visualization of GL261-luc2 mouse glioma cells by use of Alexa Fluor-labeled TRP-2 antibodies
Authors: Fenton, K. E.; Martirosyan, N. L.; Abdelwahab, M. G.; Coons, S. W.; Preul, M. C.; Scheck, A. C.
Journal: Neurosurg Focus (2014): E12
A mouse monoclonal antibody against Alexa Fluor 647
Authors: Wuethrich, I.; Guillen, E.; Ploegh, H. L.
Journal: Monoclon Antib Immunodiagn Immunother (2014): 109-20
Volume labeling with Alexa Fluor dyes and surface functionalization of highly sensitive fluorescent silica (SiO2) nanoparticles
Authors: Wang, W.; Nallathamby, P. D.; Foster, C. M.; Morrell-Falvey, J. L.; Mortensen, N. P.; Doktycz, M. J.; Gu, B.; Retterer, S. T.
Journal: Nanoscale (2013): 10369-75
Green tea catechins quench the fluorescence of bacteria-conjugated Alexa fluor dyes
Authors: Zhao, L.; Li, W.; Zhu, S.; Tsai, S.; Li, J.; Tracey, K. J.; Wang, P.; Fan, S.; Sama, A. E.; Wang, H.
Journal: Inflamm Allergy Drug Targets (2013): 308-14
Fluorescence of Alexa fluor dye tracks protein folding
Authors: Lindhoud, S.; Westphal, A. H.; Visser, A. J.; Borst, J. W.; van Mierlo, C. P.
Journal: PLoS One (2012): e46838
Visualizing dengue virus through Alexa Fluor labeling
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)