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XFD514 NHS Ester *Same Structure to Alexa Fluor™ 514 NHS Ester*

Chemical structure for XFD 514 NHS ester.
Chemical structure for XFD 514 NHS ester.
Ordering information
Price ()
Catalog Number1818
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight916.07
SolventDMSO
Spectral properties
Extinction coefficient (cm -1 M -1)80000
Excitation (nm)518
Emission (nm)543
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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XFD514 acid
XFD514 tyramide
XFD532 tyramide
XFD647 Azide
XFD647 Alkyne
XFD488 TCO
Show More (42)

OverviewpdfSDSpdfProtocol


Molecular weight
916.07
Extinction coefficient (cm -1 M -1)
80000
Excitation (nm)
518
Emission (nm)
543
XFD514 NHS ester is manufactured by AAT Bioquest, and it has the same chemical structure to Alexa Fluor® 514 NHS ester (Alexa Fluor® is the trademark of ThermoFisher). It is a bright yellow-fluorescent dye that can be used to replace rhodamine 6G with dramatically improved water solubility. XFD514 dye is water soluble and pH-insensitive from pH 4 to pH 10. The NHS ester (or succinimidyl ester) of XFD514 is the most convenient amine-reactive form for conjugating Alexa Fluor® 514 to a protein or antibody.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD514 NHS Ester *Same Structure to Alexa Fluor™ 514 NHS Ester* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM109.162 µL545.81 µL1.092 mL5.458 mL10.916 mL
5 mM21.832 µL109.162 µL218.324 µL1.092 mL2.183 mL
10 mM10.916 µL54.581 µL109.162 µL545.81 µL1.092 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)80000
Excitation (nm)518
Emission (nm)543

References


View all 40 references: Citation Explorer
Evaluation of Blood-Brain Barrier Integrity Using Vascular Permeability Markers: Evans Blue, Sodium Fluorescein, Albumin-Alexa Fluor Conjugates, and Horseradish Peroxidase.
Authors: Ahishali, Bulent and Kaya, Mehmet
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 87-103
Effects of Viscosity and Refractive Index on the Emission and Diffusion Properties of Alexa Fluor 405 Using Fluorescence Correlation and Lifetime Spectroscopies.
Authors: van Zanten, Camila and Melnikau, Dzmitry and Ryder, Alan G
Journal: Journal of fluorescence (2021): 835-845
Hot-Band Anti-Stokes Fluorescence Properties of Alexa Fluor 568.
Authors: Gajdos, Tamás and Hopp, Béla and Erdélyi, Miklós
Journal: Journal of fluorescence (2020): 437-443
Photo-isomerization of the Cyanine Dye Alexa-Fluor 647 (AF-647) in the Context of dSTORM Super-Resolution Microscopy.
Authors: Karlsson, Joshua K G and Laude, Alex and Hall, Michael J and Harriman, Anthony
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2019): 14983-14998
Observing the Reversible Single Molecule Electrochemistry of Alexa Fluor 647 Dyes by Total Internal Reflection Fluorescence Microscopy.
Authors: Fan, Sanjun and Webb, James E A and Yang, Ying and Nieves, Daniel J and Gonçales, Vinicius R and Tran, Jason and Hilzenrat, Geva and Kahram, Mohaddeseh and Tilley, Richard D and Gaus, Katharina and Gooding, J Justin
Journal: Angewandte Chemie (International ed. in English) (2019): 14495-14498
Temporal Distribution Patterns of Alexa Fluor 647-Conjugated CeNPs in the Mouse Retina After a Single Intravitreal Injection.
Authors: Wong, Lily L and Barkam, Swetha and Seal, Sudipta and McGinnis, James F
Journal: Advances in experimental medicine and biology (2019): 125-130
Photobleaching Comparison of R-Phycoerythrin-Streptavidin and Streptavidin-Alexa Fluor 568 in a Breast Cancer Cell Line.
Authors: Ostad, Seyed Nasser and Babaei, Sepideh and Bayat, Ali Ahmad and Mahmoudian, Jafar
Journal: Monoclonal antibodies in immunodiagnosis and immunotherapy (2019): 25-29
A combined solvatochromic shift and TDDFT study probing solute-solvent interactions of blue fluorescent Alexa Fluor 350 dye: Evaluation of ground and excited state dipole moments.
Authors: Patil, Mallikarjun K and Kotresh, M G and Inamdar, Sanjeev R
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2019): 142-152
Comparison between photostability of Alexa Fluor 448 and Alexa Fluor 647 with conventional dyes FITC and APC by flow cytometry.
Authors: Rai, S and Bhardwaj, U and Misra, A and Singh, S and Gupta, R
Journal: International journal of laboratory hematology (2018): e52-e54
Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye.
Authors: Winne, Johan M and Irani, Niloufer G and Van den Begin, Jos and Madder, Annemieke
Journal: Methods in molecular biology (Clifton, N.J.) (2017): 9-21