XFD660 NHS Ester [equivalent to Alexa Fluor® 660 NHS Ester]
XFD660, synthesized by AAT Bioquest, shares the exact same chemical structure with Alexa Fluor® 660 (a ThermoFisher trademark). This far-red dye is optimized for excitation by the 633 or 647 nm laser lines, making it well-suited for applications in imaging and flow cytometry where bright and photostable signal generation is critical. XFD660 is water-soluble and demonstrates pH stability within a range from 4 to 10. The NHS ester derivative of XFD660 is predominantly employed for conjugation to proteins or antibodies. This functionality allows the NHS ester to covalently attach to the primary amines present in proteins, amine-modified oligonucleotides, and other amine-bearing molecules. Conjugates utilizing XFD660 are noted for their enhanced fluorescence intensity and increased photostability relative to conjugates using other fluorophores with similar spectral characteristics.
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
XFD488 NHS Ester *Same Structure to Alexa Fluor™ 488 NHS Ester* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
XFD350 NHS Ester *Same Structure to Alexa Fluor™ 350 NHS Ester* | 343 | 441 | 19000 | - | 0.25 | 0.19 |
XFD532 NHS Ester *Same Structure to Alexa Fluor™ 532 NHS Ester* | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
XFD594 NHS Ester *Same Structure to Alexa Fluor™ 594 NHS Ester* | 590 | 618 | 90000 | 0.661 | 0.43 | 0.56 |
XFD555 NHS Ester *Same Structure to Alexa Fluor™ 555 NHS Ester* | 553 | 568 | 150000 | 0.11 | 0.08 | 0.08 |
XFD647 NHS Ester *Same Structure to Alexa Fluor™ 647 NHS Ester* | 650 | 671 | 239000 | 0.331 | 0.00 | 0.03 |
XFD680 NHS Ester *Same Structure to Alexa Fluor™ 680 NHS Ester* | 681 | 704 | 184000 | 0.361 | 0.00 | 0.05 |
XFD700 NHS Ester *Same Structure to Alexa Fluor™ 700 NHS Ester* | 696 | 719 | 192000 | 0.251 | 0.00 | 0.07 |
XFD750 NHS Ester *Same Structure to Alexa Fluor™ 750 NHS Ester* | 752 | 776 | 240000 | 0.121 | 0.00 | 0.04 |
Show More (11) |
References
View all 50 references: Citation Explorer
Enhanced fluorescence blinking of AF647 fluorophores in Mowiol via violet and UV light induced recovery for superior localization microscopy.
Authors: Bharadwaj, Anupam and Kumar, Amalesh and Mitra, Rumela and Jaganathan, Bithiah Grace and Boruah, Bosanta Ranjan
Journal: Methods and applications in fluorescence (2024)
Authors: Bharadwaj, Anupam and Kumar, Amalesh and Mitra, Rumela and Jaganathan, Bithiah Grace and Boruah, Bosanta Ranjan
Journal: Methods and applications in fluorescence (2024)
Three species multiplexing of fluorescent dyes and gold nanoclusters recovered with fluorescence lifetime correlation spectroscopy.
Authors: Kayyil Veedu, Malavika and Hajda, Agata and Olesiak-Bańska, Joanna and Wenger, Jérôme
Journal: Biochimica et biophysica acta. General subjects (2024): 130611
Authors: Kayyil Veedu, Malavika and Hajda, Agata and Olesiak-Bańska, Joanna and Wenger, Jérôme
Journal: Biochimica et biophysica acta. General subjects (2024): 130611
Vanadocene-functionalized mesoporous silica nanoparticles: platforms for the development of theranostic materials against breast cancer.
Authors: Iorhemba, Michael Aondona and Álvarez-Conde, Javier and Díaz-García, Diana and Méndez-Arriaga, José Manuel and García-Almodóvar, Victoria and Ovejero-Paredes, Karina and Idris, Sulaiman Ola and Shallangwa, Gideon Adamu and Abdulkadir, Ibrahim and Prashar, Sanjiv and Filice, Marco and Gómez-Ruiz, Santiago
Journal: Biomedical materials (Bristol, England) (2024)
Authors: Iorhemba, Michael Aondona and Álvarez-Conde, Javier and Díaz-García, Diana and Méndez-Arriaga, José Manuel and García-Almodóvar, Victoria and Ovejero-Paredes, Karina and Idris, Sulaiman Ola and Shallangwa, Gideon Adamu and Abdulkadir, Ibrahim and Prashar, Sanjiv and Filice, Marco and Gómez-Ruiz, Santiago
Journal: Biomedical materials (Bristol, England) (2024)
Amphiphilic Poly(2-oxazoline)s with Glycine-Containing Hydrophobic Blocks Tailored for Panobinostat- and Imatinib-Loaded Micelles.
Authors: Göppert, Natalie E and Quader, Sabina and Van Guyse, Joachim F R and Weber, Christine and Kataoka, Kazunori and Schubert, Ulrich S
Journal: Biomacromolecules (2023): 5915-5925
Authors: Göppert, Natalie E and Quader, Sabina and Van Guyse, Joachim F R and Weber, Christine and Kataoka, Kazunori and Schubert, Ulrich S
Journal: Biomacromolecules (2023): 5915-5925
USE OF RECOMBINANT S1 PROTEIN WITH hFc FOR ANALYSIS OF SARS-COV-2 ADSORPTION AND EVALUATION OF DRUGS THAT INHIBIT ENTRY INTO VERO E6 CELLS.
Authors: Couto, Jéssica Carla Martins and Vidal, Taís and Decker, Eduardo Reichert and Santurio, Janio M and de Mello, Carlos Fernando and Pillat, Micheli Mainardi
Journal: Immunology letters (2023)
Authors: Couto, Jéssica Carla Martins and Vidal, Taís and Decker, Eduardo Reichert and Santurio, Janio M and de Mello, Carlos Fernando and Pillat, Micheli Mainardi
Journal: Immunology letters (2023)