XFD488 NHS Ester *Same Structure to Alexa Fluor™ 488 NHS Ester*
| Overview |  SDSProtocol |
See also: Amine Reactive Dyes and Probes for Conjugation, Bioconjugation, Chemical Reagents, Dyes by Functional Group
Molecular weight 643.40 | Extinction coefficient (cm -1 M -1) 73000 | Excitation (nm) 499 | Emission (nm) 520 | Quantum yield 0.921 |
XFD488 NHS Ester (Succinimidyl Ester) is the same molecule to the Alexa Fluor® 488 NHS Ester (Alexa Fluor® is the trademark of ThermoFisher). It is a bright green-fluorescent dye optimal for use with the 488 nm Argon laser. XFD488 dye is water soluble and pH-insensitive from pH 4 to pH 10. The NHS ester (or succinimidyl ester) of XFD488 is the most convenient amine-reactive form for conjugating this dye to a protein or antibody.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of XFD488 NHS Ester *Same Structure to Alexa Fluor™ 488 NHS Ester* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 155.424 µL | 777.122 µL | 1.554 mL | 7.771 mL | 15.542 mL |
| 5 mM | 31.085 µL | 155.424 µL | 310.849 µL | 1.554 mL | 3.108 mL |
| 10 mM | 15.542 µL | 77.712 µL | 155.424 µL | 777.122 µL | 1.554 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
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Spectral properties
| Extinction coefficient (cm -1 M -1) | 73000 |
| Excitation (nm) | 499 |
| Emission (nm) | 520 |
| Quantum yield | 0.921 |
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
| XFD350 NHS Ester *Same Structure to Alexa Fluor™ 350 NHS Ester* | 343 | 441 | 19000 | - |
| XFD532 NHS Ester *Same Structure to Alexa Fluor™ 532 NHS Ester* | 534 | 553 | 81000 | 0.611 |
| XFD594 NHS Ester *Same Structure to Alexa Fluor™ 594 NHS Ester* | 590 | 618 | 92000 | 0.661 |
| XFD555 NHS Ester *Same Structure to Alexa Fluor™ 555 NHS Ester* | 553 | 568 | 155000 | 0.11 |
| XFD647 NHS Ester *Same Structure to Alexa Fluor™ 647 NHS Ester* | 650 | 671 | 270000 | 0.331 |
| XFD680 NHS Ester *Same Structure to Alexa Fluor™ 680 NHS Ester* | 681 | 704 | 132000 | 0.361 |
| XFD700 NHS Ester *Same Structure to Alexa Fluor™ 700 NHS Ester* | 696 | 719 | 205000 | 0.251 |
| XFD750 NHS Ester *Same Structure to Alexa Fluor™ 750 NHS Ester* | 752 | 776 | 290000 | 0.121 |
| XFD546 NHS Ester *Same Structure to Alexa Fluor™ 546 NHS Ester* | 561 | 572 | 112000 | 0.791 |
Show More (21) | ||||
Citations
View all 2 citations: Citation Explorer
Biophysical Characterization of Full Length TAR DNA-Binding Protein (TDP-43) Phase Separation
Authors: Staderini, Tommaso and Bigi, Alessandra and Mongiello, Daniele and Cecchi, Cristina and Chiti, Fabrizio
Journal: Protein Science (2022): e4509
Authors: Staderini, Tommaso and Bigi, Alessandra and Mongiello, Daniele and Cecchi, Cristina and Chiti, Fabrizio
Journal: Protein Science (2022): e4509
Electroretinography Changes in Feline Model of Iodoacetic Acid-induced Retinal Degeneration
Authors: Kim, Changzoo and Lee, Myungshin and Lee, Seung Uk and Lee, Sang Joon
Journal: Journal of the Korean Ophthalmological Society (2019): 1205--1215
Authors: Kim, Changzoo and Lee, Myungshin and Lee, Seung Uk and Lee, Sang Joon
Journal: Journal of the Korean Ophthalmological Society (2019): 1205--1215
References
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Authors: Velazquez-Lopez, I.; Leon-Cruz, E.; Pardo, J. P.; Sosa-Peinado, A.; Gonzalez-Andrade, M.
Journal: Anal Biochem (2017): 13-22
Authors: Velazquez-Lopez, I.; Leon-Cruz, E.; Pardo, J. P.; Sosa-Peinado, A.; Gonzalez-Andrade, M.
Journal: Anal Biochem (2017): 13-22
Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye
Authors: Winne, J. M.; Irani, N. G.; Van den Begin, J.; Madder, A.
Journal: Methods Mol Biol (2017): 21-Sep
Authors: Winne, J. M.; Irani, N. G.; Van den Begin, J.; Madder, A.
Journal: Methods Mol Biol (2017): 21-Sep
Alteration of AMPA Receptor-Mediated Synaptic Transmission by Alexa Fluor 488 and 594 in Cerebellar Stellate Cells
Authors: Maroteaux, M.; Liu, S. J.
Journal: eNeuro (2016)
Authors: Maroteaux, M.; Liu, S. J.
Journal: eNeuro (2016)
Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments
Authors: Grate, J. W.; Mo, K. F.; Shin, Y.; Vasdekis, A.; Warner, M. G.; Kelly, R. T.; Orr, G.; Hu, D.; Dehoff, K. J.; Brockman, F. J.; Wilkins, M. J.
Journal: Bioconjug Chem (2015): 593-601
Authors: Grate, J. W.; Mo, K. F.; Shin, Y.; Vasdekis, A.; Warner, M. G.; Kelly, R. T.; Orr, G.; Hu, D.; Dehoff, K. J.; Brockman, F. J.; Wilkins, M. J.
Journal: Bioconjug Chem (2015): 593-601
In vivo visualization of GL261-luc2 mouse glioma cells by use of Alexa Fluor-labeled TRP-2 antibodies
Authors: Fenton, K. E.; Martirosyan, N. L.; Abdelwahab, M. G.; Coons, S. W.; Preul, M. C.; Scheck, A. C.
Journal: Neurosurg Focus (2014): E12
Authors: Fenton, K. E.; Martirosyan, N. L.; Abdelwahab, M. G.; Coons, S. W.; Preul, M. C.; Scheck, A. C.
Journal: Neurosurg Focus (2014): E12
A mouse monoclonal antibody against Alexa Fluor 647
Authors: Wuethrich, I.; Guillen, E.; Ploegh, H. L.
Journal: Monoclon Antib Immunodiagn Immunother (2014): 109-20
Authors: Wuethrich, I.; Guillen, E.; Ploegh, H. L.
Journal: Monoclon Antib Immunodiagn Immunother (2014): 109-20
Volume labeling with Alexa Fluor dyes and surface functionalization of highly sensitive fluorescent silica (SiO2) nanoparticles
Authors: Wang, W.; Nallathamby, P. D.; Foster, C. M.; Morrell-Falvey, J. L.; Mortensen, N. P.; Doktycz, M. J.; Gu, B.; Retterer, S. T.
Journal: Nanoscale (2013): 10369-75
Authors: Wang, W.; Nallathamby, P. D.; Foster, C. M.; Morrell-Falvey, J. L.; Mortensen, N. P.; Doktycz, M. J.; Gu, B.; Retterer, S. T.
Journal: Nanoscale (2013): 10369-75
Green tea catechins quench the fluorescence of bacteria-conjugated Alexa fluor dyes
Authors: Zhao, L.; Li, W.; Zhu, S.; Tsai, S.; Li, J.; Tracey, K. J.; Wang, P.; Fan, S.; Sama, A. E.; Wang, H.
Journal: Inflamm Allergy Drug Targets (2013): 308-14
Authors: Zhao, L.; Li, W.; Zhu, S.; Tsai, S.; Li, J.; Tracey, K. J.; Wang, P.; Fan, S.; Sama, A. E.; Wang, H.
Journal: Inflamm Allergy Drug Targets (2013): 308-14
Fluorescence of Alexa fluor dye tracks protein folding
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Journal: PLoS One (2012): e46838
Authors: Lindhoud, S.; Westphal, A. H.; Visser, A. J.; Borst, J. W.; van Mierlo, C. P.
Journal: PLoS One (2012): e46838
Visualizing dengue virus through Alexa Fluor labeling
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)
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