XFD488 NHS Ester *Same Structure to Alexa Fluor™ 488 NHS Ester*
XFD488 NHS Ester (Succinimidyl Ester) is the same molecule to the Alexa Fluor® 488 NHS Ester (Alexa Fluor® is the trademark of ThermoFisher). It is a bright green-fluorescent dye optimal for use with the 488 nm Argon laser. XFD488 dye is water soluble and pH-insensitive from pH 4 to pH 10. The NHS ester (or succinimidyl ester) of XFD488 is the most convenient amine-reactive form for conjugating this dye to a protein or antibody.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of XFD488 NHS Ester *Same Structure to Alexa Fluor™ 488 NHS Ester* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 155.424 µL | 777.122 µL | 1.554 mL | 7.771 mL | 15.542 mL |
5 mM | 31.085 µL | 155.424 µL | 310.849 µL | 1.554 mL | 3.108 mL |
10 mM | 15.542 µL | 77.712 µL | 155.424 µL | 777.122 µL | 1.554 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
XFD350 NHS Ester *Same Structure to Alexa Fluor™ 350 NHS Ester* | 343 | 441 | 19000 | 0.25 | 0.19 |
XFD532 NHS Ester *Same Structure to Alexa Fluor™ 532 NHS Ester* | 534 | 553 | 81000 | 0.24 | 0.09 |
XFD594 NHS Ester *Same Structure to Alexa Fluor™ 594 NHS Ester* | 590 | 618 | 90000 | 0.43 | 0.56 |
XFD555 NHS Ester *Same Structure to Alexa Fluor™ 555 NHS Ester* | 553 | 568 | 150000 | 0.08 | 0.08 |
XFD647 NHS Ester *Same Structure to Alexa Fluor™ 647 NHS Ester* | 650 | 671 | 239000 | 0.00 | 0.03 |
XFD680 NHS Ester *Same Structure to Alexa Fluor™ 680 NHS Ester* | 681 | 704 | 184000 | 0.00 | 0.05 |
XFD700 NHS Ester *Same Structure to Alexa Fluor™ 700 NHS Ester* | 696 | 719 | 192000 | 0.00 | 0.07 |
XFD750 NHS Ester *Same Structure to Alexa Fluor™ 750 NHS Ester* | 752 | 776 | 240000 | 0.00 | 0.04 |
XFD546 NHS Ester *Same Structure to Alexa Fluor™ 546 NHS Ester* | 561 | 572 | 112000 | 0.21 | 0.12 |
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Citations
View all 5 citations: Citation Explorer
Biophysical characterization of the phase separation of TDP-43 devoid of the C-terminal domain
Authors: Staderini, Tommaso and Bigi, Alessandra and Lagr{\`e}ve, Cl{\'e}ment and Marzi, Isabella and Bemporad, Francesco and Chiti, Fabrizio
Journal: Cellular \& Molecular Biology Letters (2024): 104
Authors: Staderini, Tommaso and Bigi, Alessandra and Lagr{\`e}ve, Cl{\'e}ment and Marzi, Isabella and Bemporad, Francesco and Chiti, Fabrizio
Journal: Cellular \& Molecular Biology Letters (2024): 104
A rational designed multi-epitope vaccine elicited robust protective efficacy against Klebsiella pneumoniae lung infection
Authors: Liao, Jingwen and Zhang, Xiaoli and Zeng, Xi and Zhao, Zhuo and Sun, Tianjun and Xia, Zhenping and Jing, Haiming and Yuan, Yue and Chen, Zhifu and Gou, Qiang and others,
Journal: Biomedicine \& Pharmacotherapy (2024): 116611
Authors: Liao, Jingwen and Zhang, Xiaoli and Zeng, Xi and Zhao, Zhuo and Sun, Tianjun and Xia, Zhenping and Jing, Haiming and Yuan, Yue and Chen, Zhifu and Gou, Qiang and others,
Journal: Biomedicine \& Pharmacotherapy (2024): 116611
In vitro cytocompatibility of antibacterial silver and copper-doped bioactive glasses
Authors: Lallukka, Mari and Houaoui, Amel and Miola, Marta and Miettinen, Susanna and Massera, Jonathan and Vern{\'e}, Enrica
Journal: Ceramics International (2023)
Authors: Lallukka, Mari and Houaoui, Amel and Miola, Marta and Miettinen, Susanna and Massera, Jonathan and Vern{\'e}, Enrica
Journal: Ceramics International (2023)
Biophysical Characterization of Full Length TAR DNA-Binding Protein (TDP-43) Phase Separation
Authors: Staderini, Tommaso and Bigi, Alessandra and Mongiello, Daniele and Cecchi, Cristina and Chiti, Fabrizio
Journal: Protein Science (2022): e4509
Authors: Staderini, Tommaso and Bigi, Alessandra and Mongiello, Daniele and Cecchi, Cristina and Chiti, Fabrizio
Journal: Protein Science (2022): e4509
Electroretinography Changes in Feline Model of Iodoacetic Acid-induced Retinal Degeneration
Authors: Kim, Changzoo and Lee, Myungshin and Lee, Seung Uk and Lee, Sang Joon
Journal: Journal of the Korean Ophthalmological Society (2019): 1205--1215
Authors: Kim, Changzoo and Lee, Myungshin and Lee, Seung Uk and Lee, Sang Joon
Journal: Journal of the Korean Ophthalmological Society (2019): 1205--1215
References
View all 39 references: Citation Explorer
Development of new hCaM-Alexa Fluor(R) biosensors for a wide range of ligands
Authors: Velazquez-Lopez, I.; Leon-Cruz, E.; Pardo, J. P.; Sosa-Peinado, A.; Gonzalez-Andrade, M.
Journal: Anal Biochem (2017): 13-22
Authors: Velazquez-Lopez, I.; Leon-Cruz, E.; Pardo, J. P.; Sosa-Peinado, A.; Gonzalez-Andrade, M.
Journal: Anal Biochem (2017): 13-22
Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye
Authors: Winne, J. M.; Irani, N. G.; Van den Begin, J.; Madder, A.
Journal: Methods Mol Biol (2017): 21-Sep
Authors: Winne, J. M.; Irani, N. G.; Van den Begin, J.; Madder, A.
Journal: Methods Mol Biol (2017): 21-Sep
Alteration of AMPA Receptor-Mediated Synaptic Transmission by Alexa Fluor 488 and 594 in Cerebellar Stellate Cells
Authors: Maroteaux, M.; Liu, S. J.
Journal: eNeuro (2016)
Authors: Maroteaux, M.; Liu, S. J.
Journal: eNeuro (2016)
Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments
Authors: Grate, J. W.; Mo, K. F.; Shin, Y.; Vasdekis, A.; Warner, M. G.; Kelly, R. T.; Orr, G.; Hu, D.; Dehoff, K. J.; Brockman, F. J.; Wilkins, M. J.
Journal: Bioconjug Chem (2015): 593-601
Authors: Grate, J. W.; Mo, K. F.; Shin, Y.; Vasdekis, A.; Warner, M. G.; Kelly, R. T.; Orr, G.; Hu, D.; Dehoff, K. J.; Brockman, F. J.; Wilkins, M. J.
Journal: Bioconjug Chem (2015): 593-601
A mouse monoclonal antibody against Alexa Fluor 647
Authors: Wuethrich, I.; Guillen, E.; Ploegh, H. L.
Journal: Monoclon Antib Immunodiagn Immunother (2014): 109-20
Authors: Wuethrich, I.; Guillen, E.; Ploegh, H. L.
Journal: Monoclon Antib Immunodiagn Immunother (2014): 109-20