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Cy3B NHS ester

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Physical properties
Molecular weight657.74
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.048
Correction Factor (280 nm)0.069
Extinction coefficient (cm -1 M -1)1200001
Excitation (nm)560
Emission (nm)571
Quantum yield0.581
Storage, safety and handling
Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C); Minimize light exposure

OverviewpdfSDSpdfProtocol


Molecular weight
657.74
Correction Factor (260 nm)
0.048
Correction Factor (280 nm)
0.069
Extinction coefficient (cm -1 M -1)
1200001
Excitation (nm)
560
Emission (nm)
571
Quantum yield
0.581
Cy3® dye is one of the most common cyanine dyes used for labeling proteins, nucleic acids and other biological molecules. A variety of Cy3® dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. Cy3 is the least fluorescent dyes among all the Cy dyes. Cy3B is an improved version of Cy3 dyes with significantly increased fluorescence quantum yield and photostability. Cy3® is the trademark of GE Healthcare.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cy3B NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM152.036 µL760.179 µL1.52 mL7.602 mL15.204 mL
5 mM30.407 µL152.036 µL304.072 µL1.52 mL3.041 mL
10 mM15.204 µL76.018 µL152.036 µL760.179 µL1.52 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.048
Correction Factor (280 nm)0.069
Extinction coefficient (cm -1 M -1)1200001
Excitation (nm)560
Emission (nm)571
Quantum yield0.581

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
Cy3DIGE NHS ester55556915000010.070.073
XFD488 NHS Ester *Same Structure to Alexa Fluor™ 488 NHS Ester*499520710000.300.11
XFD350 NHS Ester *Same Structure to Alexa Fluor™ 350 NHS Ester*343441190000.250.19
XFD532 NHS Ester *Same Structure to Alexa Fluor™ 532 NHS Ester*534553810000.240.09
XFD594 NHS Ester *Same Structure to Alexa Fluor™ 594 NHS Ester*590618900000.430.56
QXY21 NHS ester [equivalent to QSY-21 NHS ester]--890001-0.32
XFD555 NHS Ester *Same Structure to Alexa Fluor™ 555 NHS Ester*5535681500000.080.08
XFD647 NHS Ester *Same Structure to Alexa Fluor™ 647 NHS Ester*6506712390000.000.03
XFD680 NHS Ester *Same Structure to Alexa Fluor™ 680 NHS Ester*6817041840000.000.05
XFD700 NHS Ester *Same Structure to Alexa Fluor™ 700 NHS Ester*6967191920000.000.07
XFD750 NHS Ester *Same Structure to Alexa Fluor™ 750 NHS Ester*7527762400000.000.04
Cy5DIGE NHS ester65167025000010.020.03
Cy2DIGE NHS ester4925081500000.080.15
XFD546 NHS Ester *Same Structure to Alexa Fluor™ 546 NHS Ester*5615721120000.210.12
XFD568 NHS Ester *Same Structure to Alexa Fluor™ 568 NHS Ester*579603913000.450.46
XFD514 NHS Ester *Same Structure to Alexa Fluor™ 514 NHS Ester*518543800000.310.18
QXY7 NHS ester [equivalent to QSY-7 NHS ester]--900001-0.22
CypHer5E NHS Ester643660---
CypHer7E NHS Ester748769---
XFD660 NHS Ester [equivalent to Alexa Fluor® 660 NHS Ester]663691132000--
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Citations


View all 3 citations: Citation Explorer
Homogeneous fluorescence anisotropy-based assay for characterization of ligand binding dynamics to GPCRs in budded baculoviruses: the case of Cy3B-NDP-alpha-MSH binding to MC4 receptors
Authors: Veiksina, S., Kopanchuk, S., Mazina, O., Link, R., Lille, A., Rinken, A.
Journal: Methods Mol Biol (2015): 37-50
A highly fluorescent DNA toolkit: synthesis and properties of oligonucleotides containing new Cy3, Cy5 and Cy3B monomers
Authors: Hall, L. M., Gerowska, M., Brown, T.
Journal: Nucleic Acids Res (2012): e108
Cy3B: improving the performance of cyanine dyes
Authors: Cooper, M., Ebner, A., Briggs, M., Burrows, M., Gardner, N., Richardson, R., West, R.
Journal: J Fluoresc (2004): 145-50