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Cy3B NHS ester
Cy3® dye is one of the most common cyanine dyes used for labeling proteins, nucleic acids and other biological molecules. A variety of Cy3® dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. Cy3 is the least fluorescent dyes among all the Cy dyes. Cy3B is an improved version of Cy3 dyes with significantly increased fluorescence quantum yield and photostability. Cy3® is the trademark of GE Healthcare.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cy3B NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 152.036 µL | 760.179 µL | 1.52 mL | 7.602 mL | 15.204 mL |
| 5 mM | 30.407 µL | 152.036 µL | 304.072 µL | 1.52 mL | 3.041 mL |
| 10 mM | 15.204 µL | 76.018 µL | 152.036 µL | 760.179 µL | 1.52 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Cy3DIGE NHS ester | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
| Cy2DIGE NHS ester | 492 | 508 | 150000 | 0.120 | 0.08 | 0.15 |
| Cy5DIGE NHS ester | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
| CypHer5E NHS Ester | 643 | 660 | - | - | - | - |
| CypHer7E NHS Ester | 748 | 769 | - | - | - | - |
| QXY21 NHS ester [equivalent to QSY-21 NHS ester] | - | - | 890001 | - | - | 0.32 |
| QXY7 NHS ester [equivalent to QSY-7 NHS ester] | - | - | 900001 | - | - | 0.22 |
| XFD350 NHS Ester *Same Structure to Alexa Fluor™ 350 NHS Ester* | 343 | 441 | 19000 | - | 0.25 | 0.19 |
| XFD430 NHS ester | 432 | 540 | 15,000 | - | - | 0.28 |
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Citations
View all 3 citations: Citation Explorer
Homogeneous fluorescence anisotropy-based assay for characterization of ligand binding dynamics to GPCRs in budded baculoviruses: the case of Cy3B-NDP-alpha-MSH binding to MC4 receptors
Authors: Veiksina, S., Kopanchuk, S., Mazina, O., Link, R., Lille, A., Rinken, A.
Journal: Methods Mol Biol (2015): 37-50
Authors: Veiksina, S., Kopanchuk, S., Mazina, O., Link, R., Lille, A., Rinken, A.
Journal: Methods Mol Biol (2015): 37-50
A highly fluorescent DNA toolkit: synthesis and properties of oligonucleotides containing new Cy3, Cy5 and Cy3B monomers
Authors: Hall, L. M., Gerowska, M., Brown, T.
Journal: Nucleic Acids Res (2012): e108
Authors: Hall, L. M., Gerowska, M., Brown, T.
Journal: Nucleic Acids Res (2012): e108
Cy3B: improving the performance of cyanine dyes
Authors: Cooper, M., Ebner, A., Briggs, M., Burrows, M., Gardner, N., Richardson, R., West, R.
Journal: J Fluoresc (2004): 145-50
Authors: Cooper, M., Ebner, A., Briggs, M., Burrows, M., Gardner, N., Richardson, R., West, R.
Journal: J Fluoresc (2004): 145-50
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