XFD350 NHS Ester Same Structure to Alexa Fluor™ 350 NHS Ester

XFD350 NHS Ester (Succinimidyl Ester) is the same molecule to the Alexa Fluor® 350 NHS Ester (Alexa Fluor® is the trademark of ThermoFisher). It is a blue-fluorescent dye optimal for use with the 350 nm UV laser. XFD350 dye is water soluble and pH-insensitive from pH 4 to pH 10. The NHS ester (or succinimidyl ester) of XFD350 is the most convenient amine-reactive form for conjugating this dye to a protein or antibody.

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
XFD488 NHS Ester Same Structure to Alexa Fluor™ 488 NHS Ester	499	520	71000	0.92¹	0.30	0.11
XFD532 NHS Ester Same Structure to Alexa Fluor™ 532 NHS Ester	534	553	81000	0.61¹	0.24	0.09
XFD594 NHS Ester Same Structure to Alexa Fluor™ 594 NHS Ester	590	618	90000	0.66¹	0.43	0.56
XFD555 NHS Ester Same Structure to Alexa Fluor™ 555 NHS Ester	553	568	150000	0.1¹	0.08	0.08
XFD647 NHS Ester Same Structure to Alexa Fluor™ 647 NHS Ester	650	671	239000	0.33¹	0.00	0.03
XFD680 NHS Ester Same Structure to Alexa Fluor™ 680 NHS Ester	681	704	184000	0.36¹	0.00	0.05
XFD700 NHS Ester Same Structure to Alexa Fluor™ 700 NHS Ester	696	719	192000	0.25¹	0.00	0.07
XFD750 NHS Ester Same Structure to Alexa Fluor™ 750 NHS Ester	752	776	240000	0.12¹	0.00	0.04
XFD546 NHS Ester Same Structure to Alexa Fluor™ 546 NHS Ester	561	572	112000	0.79¹	0.21	0.12
XFD568 NHS Ester Same Structure to Alexa Fluor™ 568 NHS Ester	579	603	91300	0.69¹	0.45	0.46
XFD514 NHS Ester Same Structure to Alexa Fluor™ 514 NHS Ester	518	543	80000	-	0.31	0.18
XFD660 NHS Ester [equivalent to Alexa Fluor® 660 NHS Ester]	663	691	132000	-	-	-
QXY21 NHS ester [equivalent to QSY-21 NHS ester]	-	-	89000¹	-	-	0.32
Cy5DIGE NHS ester	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
Cy2DIGE NHS ester	492	508	150000	0.120	0.08	0.15
Cy3DIGE NHS ester	555	569	150000¹	0.15¹	0.07	0.073
QXY7 NHS ester [equivalent to QSY-7 NHS ester]	-	-	90000¹	-	-	0.22
Cy3B NHS ester	560	571	120000¹	0.58¹	0.048	0.069
CypHer5E NHS Ester	643	660	-	-	-	-
CypHer7E NHS Ester	748	769	-	-	-	-
Show More (11)

Citations

View all 2 citations: Citation Explorer

A combined solvatochromic shift and TDDFT study probing solute-solvent interactions of blue fluorescent Alexa Fluor 350 dye: Evaluation of ground and excited state dipole moments
Authors: Patil, M. K., Kotresh, M. G., Inamdar, S. R.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2019): 142-152

Observing the Reversible Single Molecule Electrochemistry of Alexa Fluor 647 Dyes by Total Internal Reflection Fluorescence Microscopy
Authors: Fan, S., Webb, J. E. A., Yang, Y., Nieves, D. J., Goncales, V. R., Tran, J., Hilzenrat, G., Kahram, M., Tilley, R. D., Gaus, K., Gooding, J. J.
Journal: Angew Chem Int Ed Engl (2019): 14495-14498

References

View all 39 references: Citation Explorer

Development of new hCaM-Alexa Fluor(R) biosensors for a wide range of ligands
Authors: Velazquez-Lopez, I.; Leon-Cruz, E.; Pardo, J. P.; Sosa-Peinado, A.; Gonzalez-Andrade, M.
Journal: Anal Biochem (2017): 13-22

Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye
Authors: Winne, J. M.; Irani, N. G.; Van den Begin, J.; Madder, A.
Journal: Methods Mol Biol (2017): 21-Sep

Alteration of AMPA Receptor-Mediated Synaptic Transmission by Alexa Fluor 488 and 594 in Cerebellar Stellate Cells
Authors: Maroteaux, M.; Liu, S. J.
Journal: eNeuro (2016)

Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments
Authors: Grate, J. W.; Mo, K. F.; Shin, Y.; Vasdekis, A.; Warner, M. G.; Kelly, R. T.; Orr, G.; Hu, D.; Dehoff, K. J.; Brockman, F. J.; Wilkins, M. J.
Journal: Bioconjug Chem (2015): 593-601

In vivo visualization of GL261-luc2 mouse glioma cells by use of Alexa Fluor-labeled TRP-2 antibodies
Authors: Fenton, K. E.; Martirosyan, N. L.; Abdelwahab, M. G.; Coons, S. W.; Preul, M. C.; Scheck, A. C.
Journal: Neurosurg Focus (2014): E12

Page updated on September 19, 2024

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Physical properties

Molecular weight	511.55
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.25
Correction Factor (280 nm)	0.19
Extinction coefficient (cm ^-1 M ^-1)	19000
Excitation (nm)	343
Emission (nm)	441

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501