XFD546 NHS Ester Same Structure to Alexa Fluor™ 546 NHS Ester

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Physical properties

Molecular weight	1159.60
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.21
Correction Factor (280 nm)	0.12
Extinction coefficient (cm ^-1 M ^-1)	112000
Excitation (nm)	323
Emission (nm)	572
Quantum yield	0.79¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

1159.60

Correction Factor (260 nm)

0.21

Correction Factor (280 nm)

0.12

Extinction coefficient (cm ^-1 M ^-1)

112000

Excitation (nm)

323

Emission (nm)

572

Quantum yield

0.79¹

XFD546 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 546 (Alexa Fluor® is the trademark of ThermoFisher). XFD546 NHS Ester (Succinimidyl Ester) has the same molecule as the Alexa Fluor® 546 NHS Ester. It is a bright orange-fluorescent dye optimal for use with the frequency-doubled Nd:YAG laser line. XFD546 dye is water soluble and pH-insensitive from pH 4 to pH 10. The NHS ester (or succinimidyl ester) of XFD546 is the most convenient amine-reactive form for conjugating this dye to a protein or antibody.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD546 NHS Ester *Same Structure to Alexa Fluor™ 546 NHS Ester* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	86.237 µL	431.183 µL	862.366 µL	4.312 mL	8.624 mL
5 mM	17.247 µL	86.237 µL	172.473 µL	862.366 µL	1.725 mL
10 mM	8.624 µL	43.118 µL	86.237 µL	431.183 µL	862.366 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.21
Correction Factor (280 nm)	0.12
Extinction coefficient (cm ^-1 M ^-1)	112000
Excitation (nm)	323
Emission (nm)	572
Quantum yield	0.79¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
XFD488 NHS Ester Same Structure to Alexa Fluor™ 488 NHS Ester	490	520	71000	0.92¹	0.30	0.11
XFD350 NHS Ester Same Structure to Alexa Fluor™ 350 NHS Ester	303	439	19000	-	0.25	0.19
XFD532 NHS Ester Same Structure to Alexa Fluor™ 532 NHS Ester	317	553	81000	0.61¹	0.24	0.09
XFD594 NHS Ester Same Structure to Alexa Fluor™ 594 NHS Ester	375	618	90000	0.66¹	0.43	0.56
XFD555 NHS Ester Same Structure to Alexa Fluor™ 555 NHS Ester	520	568	150000	0.1¹	0.08	0.08
XFD647 NHS Ester Same Structure to Alexa Fluor™ 647 NHS Ester	604	671	239000	0.33¹	0.00	0.03
XFD680 NHS Ester Same Structure to Alexa Fluor™ 680 NHS Ester	681	704	184000	0.36¹	0.00	0.05
XFD700 NHS Ester Same Structure to Alexa Fluor™ 700 NHS Ester	696	719	192000	0.25¹	0.00	0.07
XFD750 NHS Ester Same Structure to Alexa Fluor™ 750 NHS Ester	752	776	240000	0.12¹	0.00	0.04
XFD568 NHS Ester Same Structure to Alexa Fluor™ 568 NHS Ester	546	603	91300	0.69¹	0.45	0.46
XFD514 NHS Ester Same Structure to Alexa Fluor™ 514 NHS Ester	315	543	80000	-	0.31	0.18
QXY21 NHS ester [equivalent to QSY-21 NHS ester]	-	-	89000¹	-	-	0.32
Cy5DIGE NHS ester	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
Cy2DIGE NHS ester	492	508	150000	0.120	0.08	0.15
Cy3DIGE NHS ester	555	569	150000¹	0.15¹	0.07	0.073
QXY7 NHS ester [equivalent to QSY-7 NHS ester]	-	-	90000¹	-	-	0.22
Cy3B NHS ester	560	571	120000¹	0.58¹	0.048	0.069
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Citations

View all 2 citations: Citation Explorer

A combined solvatochromic shift and TDDFT study probing solute-solvent interactions of blue fluorescent Alexa Fluor 350 dye: Evaluation of ground and excited state dipole moments
Authors: Patil, M. K., Kotresh, M. G., Inamdar, S. R.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2019): 142-152

Observing the Reversible Single Molecule Electrochemistry of Alexa Fluor 647 Dyes by Total Internal Reflection Fluorescence Microscopy
Authors: Fan, S., Webb, J. E. A., Yang, Y., Nieves, D. J., Goncales, V. R., Tran, J., Hilzenrat, G., Kahram, M., Tilley, R. D., Gaus, K., Gooding, J. J.
Journal: Angew Chem Int Ed Engl (2019): 14495-14498

References

View all 1 references: Citation Explorer

Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: fluorescence of the dyes and their bioconjugates
Authors: Berlier JE, Rothe A, Buller G, Bradford J, Gray DR, Filanoski BJ, Telford WG, Yue S, Liu J, Cheung CY, Chang W, Hirsch JD, Beechem JM, Haugl and RP., undefined
Journal: J Histochem Cytochem (2003): 1699