XFD546 NHS Ester *Same Structure to Alexa Fluor™ 546 NHS Ester*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1159.60 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.12 |
Extinction coefficient (cm -1 M -1) | 112000 |
Excitation (nm) | 323 |
Emission (nm) | 572 |
Quantum yield | 0.791 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | ![]() ![]() |
Molecular weight 1159.60 | Correction Factor (260 nm) 0.21 | Correction Factor (280 nm) 0.12 | Extinction coefficient (cm -1 M -1) 112000 | Excitation (nm) 323 | Emission (nm) 572 | Quantum yield 0.791 |
XFD546 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 546 (Alexa Fluor® is the trademark of ThermoFisher). XFD546 NHS Ester (Succinimidyl Ester) has the same molecule as the Alexa Fluor® 546 NHS Ester. It is a bright orange-fluorescent dye optimal for use with the frequency-doubled Nd:YAG laser line. XFD546 dye is water soluble and pH-insensitive from pH 4 to pH 10. The NHS ester (or succinimidyl ester) of XFD546 is the most convenient amine-reactive form for conjugating this dye to a protein or antibody.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of XFD546 NHS Ester *Same Structure to Alexa Fluor™ 546 NHS Ester* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 86.237 µL | 431.183 µL | 862.366 µL | 4.312 mL | 8.624 mL |
5 mM | 17.247 µL | 86.237 µL | 172.473 µL | 862.366 µL | 1.725 mL |
10 mM | 8.624 µL | 43.118 µL | 86.237 µL | 431.183 µL | 862.366 µL |
Molarity calculator
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.12 |
Extinction coefficient (cm -1 M -1) | 112000 |
Excitation (nm) | 323 |
Emission (nm) | 572 |
Quantum yield | 0.791 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
XFD488 NHS Ester *Same Structure to Alexa Fluor™ 488 NHS Ester* | 490 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
XFD350 NHS Ester *Same Structure to Alexa Fluor™ 350 NHS Ester* | 303 | 439 | 19000 | - | 0.25 | 0.19 |
XFD532 NHS Ester *Same Structure to Alexa Fluor™ 532 NHS Ester* | 317 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
XFD594 NHS Ester *Same Structure to Alexa Fluor™ 594 NHS Ester* | 375 | 618 | 90000 | 0.661 | 0.43 | 0.56 |
XFD555 NHS Ester *Same Structure to Alexa Fluor™ 555 NHS Ester* | 520 | 568 | 150000 | 0.11 | 0.08 | 0.08 |
XFD647 NHS Ester *Same Structure to Alexa Fluor™ 647 NHS Ester* | 604 | 671 | 239000 | 0.331 | 0.00 | 0.03 |
XFD680 NHS Ester *Same Structure to Alexa Fluor™ 680 NHS Ester* | 681 | 704 | 184000 | 0.361 | 0.00 | 0.05 |
XFD700 NHS Ester *Same Structure to Alexa Fluor™ 700 NHS Ester* | 696 | 719 | 192000 | 0.251 | 0.00 | 0.07 |
XFD750 NHS Ester *Same Structure to Alexa Fluor™ 750 NHS Ester* | 752 | 776 | 240000 | 0.121 | 0.00 | 0.04 |
Show More (8) |
Citations
View all 2 citations: Citation Explorer
A combined solvatochromic shift and TDDFT study probing solute-solvent interactions of blue fluorescent Alexa Fluor 350 dye: Evaluation of ground and excited state dipole moments
Authors: Patil, M. K., Kotresh, M. G., Inamdar, S. R.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2019): 142-152
Authors: Patil, M. K., Kotresh, M. G., Inamdar, S. R.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2019): 142-152
Observing the Reversible Single Molecule Electrochemistry of Alexa Fluor 647 Dyes by Total Internal Reflection Fluorescence Microscopy
Authors: Fan, S., Webb, J. E. A., Yang, Y., Nieves, D. J., Goncales, V. R., Tran, J., Hilzenrat, G., Kahram, M., Tilley, R. D., Gaus, K., Gooding, J. J.
Journal: Angew Chem Int Ed Engl (2019): 14495-14498
Authors: Fan, S., Webb, J. E. A., Yang, Y., Nieves, D. J., Goncales, V. R., Tran, J., Hilzenrat, G., Kahram, M., Tilley, R. D., Gaus, K., Gooding, J. J.
Journal: Angew Chem Int Ed Engl (2019): 14495-14498
References
View all 1 references: Citation Explorer
Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: fluorescence of the dyes and their bioconjugates
Authors: Berlier JE, Rothe A, Buller G, Bradford J, Gray DR, Filanoski BJ, Telford WG, Yue S, Liu J, Cheung CY, Chang W, Hirsch JD, Beechem JM, Haugl and RP., undefined
Journal: J Histochem Cytochem (2003): 1699
Authors: Berlier JE, Rothe A, Buller G, Bradford J, Gray DR, Filanoski BJ, Telford WG, Yue S, Liu J, Cheung CY, Chang W, Hirsch JD, Beechem JM, Haugl and RP., undefined
Journal: J Histochem Cytochem (2003): 1699
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)