XFD568 NHS Ester *Same Structure to Alexa Fluor™ 568 NHS Ester*
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See also: Amine Reactive Dyes and Probes for Conjugation, Bioconjugation, Chemical Reagents, Dyes by Functional Group
CAS 247145-38-6 | Molecular weight 791.80 | Extinction coefficient (cm -1 M -1) 88000 | Excitation (nm) 579 | Emission (nm) 603 | Quantum yield 0.691 |
XFD568 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 568 (Alexa Fluor® is the trademark of ThermoFisher). It is a bright orange-fluorescent dye optimal for use with the frequency-doubled Nd:YAG laser line. XFD568 dye is water soluble and pH-insensitive from pH 4 to pH 10. The NHS ester (or succinimidyl ester) of XFD568 is the most convenient amine-reactive form for conjugating this dye to a protein or antibody.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of XFD568 NHS Ester *Same Structure to Alexa Fluor™ 568 NHS Ester* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 126.295 µL | 631.473 µL | 1.263 mL | 6.315 mL | 12.629 mL |
| 5 mM | 25.259 µL | 126.295 µL | 252.589 µL | 1.263 mL | 2.526 mL |
| 10 mM | 12.629 µL | 63.147 µL | 126.295 µL | 631.473 µL | 1.263 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
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Spectral properties
| Extinction coefficient (cm -1 M -1) | 88000 |
| Excitation (nm) | 579 |
| Emission (nm) | 603 |
| Quantum yield | 0.691 |
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
| XFD488 NHS Ester *Same Structure to Alexa Fluor™ 488 NHS Ester* | 499 | 520 | 73000 | 0.921 |
| XFD350 NHS Ester *Same Structure to Alexa Fluor™ 350 NHS Ester* | 343 | 441 | 19000 | - |
| XFD532 NHS Ester *Same Structure to Alexa Fluor™ 532 NHS Ester* | 534 | 553 | 81000 | 0.611 |
| XFD594 NHS Ester *Same Structure to Alexa Fluor™ 594 NHS Ester* | 590 | 618 | 92000 | 0.661 |
| XFD555 NHS Ester *Same Structure to Alexa Fluor™ 555 NHS Ester* | 553 | 568 | 155000 | 0.11 |
| XFD647 NHS Ester *Same Structure to Alexa Fluor™ 647 NHS Ester* | 650 | 671 | 270000 | 0.331 |
| XFD680 NHS Ester *Same Structure to Alexa Fluor™ 680 NHS Ester* | 681 | 704 | 132000 | 0.361 |
| XFD700 NHS Ester *Same Structure to Alexa Fluor™ 700 NHS Ester* | 696 | 719 | 205000 | 0.251 |
| XFD750 NHS Ester *Same Structure to Alexa Fluor™ 750 NHS Ester* | 752 | 776 | 290000 | 0.121 |
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Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
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FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)