XFD568 NHS Ester Same Structure to Alexa Fluor™ 568 NHS Ester

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	791.80
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.45
Correction Factor (280 nm)	0.46
Extinction coefficient (cm ^-1 M ^-1)	91300
Excitation (nm)	579
Emission (nm)	603
Quantum yield	0.69¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

247145-38-6

Molecular weight

791.80

Correction Factor (260 nm)

0.45

Correction Factor (280 nm)

0.46

Extinction coefficient (cm ^-1 M ^-1)

91300

Excitation (nm)

579

Emission (nm)

603

Quantum yield

0.69¹

XFD568 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 568 (Alexa Fluor® is the trademark of ThermoFisher). It is a bright orange-fluorescent dye optimal for use with the frequency-doubled Nd:YAG laser line. XFD568 dye is water soluble and pH-insensitive from pH 4 to pH 10. The NHS ester (or succinimidyl ester) of XFD568 is the most convenient amine-reactive form for conjugating this dye to a protein or antibody.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD568 NHS Ester *Same Structure to Alexa Fluor™ 568 NHS Ester* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	126.295 µL	631.473 µL	1.263 mL	6.315 mL	12.629 mL
5 mM	25.259 µL	126.295 µL	252.589 µL	1.263 mL	2.526 mL
10 mM	12.629 µL	63.147 µL	126.295 µL	631.473 µL	1.263 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Spectral properties

Correction Factor (260 nm)	0.45
Correction Factor (280 nm)	0.46
Extinction coefficient (cm ^-1 M ^-1)	91300
Excitation (nm)	579
Emission (nm)	603
Quantum yield	0.69¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
XFD488 NHS Ester Same Structure to Alexa Fluor™ 488 NHS Ester	499	520	71000	0.92¹	0.30	0.11
XFD350 NHS Ester Same Structure to Alexa Fluor™ 350 NHS Ester	343	441	19000	-	0.25	0.19
XFD532 NHS Ester Same Structure to Alexa Fluor™ 532 NHS Ester	534	553	81000	0.61¹	0.24	0.09
XFD594 NHS Ester Same Structure to Alexa Fluor™ 594 NHS Ester	590	618	90000	0.66¹	0.43	0.56
XFD555 NHS Ester Same Structure to Alexa Fluor™ 555 NHS Ester	553	568	150000	0.1¹	0.08	0.08
XFD647 NHS Ester Same Structure to Alexa Fluor™ 647 NHS Ester	650	671	239000	0.33¹	0.00	0.03
XFD680 NHS Ester Same Structure to Alexa Fluor™ 680 NHS Ester	681	704	184000	0.36¹	0.00	0.05
XFD700 NHS Ester Same Structure to Alexa Fluor™ 700 NHS Ester	696	719	192000	0.25¹	0.00	0.07
XFD750 NHS Ester Same Structure to Alexa Fluor™ 750 NHS Ester	752	776	240000	0.12¹	0.00	0.04
XFD546 NHS Ester Same Structure to Alexa Fluor™ 546 NHS Ester	561	572	112000	0.79¹	0.21	0.12
XFD514 NHS Ester Same Structure to Alexa Fluor™ 514 NHS Ester	518	543	80000	-	0.31	0.18
QXY21 NHS ester [equivalent to QSY-21 NHS ester]	-	-	89000¹	-	-	0.32
Cy5DIGE NHS ester	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
Cy2DIGE NHS ester	492	508	150000	0.120	0.08	0.15
Cy3DIGE NHS ester	555	569	150000¹	0.15¹	0.07	0.073
QXY7 NHS ester [equivalent to QSY-7 NHS ester]	-	-	90000¹	-	-	0.22
Cy3B NHS ester	560	571	120000¹	0.58¹	0.048	0.069
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