QXY7 NHS ester [equivalent to QSY-7 NHS ester]

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Physical properties

Molecular weight	791.32
Solvent	DMSO

Spectral properties

Absorbance (nm)	561
Correction Factor (280 nm)	0.22
Extinction coefficient (cm ^-1 M ^-1)	90000¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

791.32

Absorbance (nm)

561

Correction Factor (280 nm)

0.22

Extinction coefficient (cm ^-1 M ^-1)

90000¹

QXY7 is the same molecule to QSY-7 acid (ThermoFisher). QXY7 has a broad and intense absorption at ~550 nm maxima with no detectable fluorescence, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications paring with Cy3, TAMRA, Alexa Fluor 555 and iFluor 647 or other spectrally similar fluorescent dyes. QXY7 NHS ester is quite stable and readily reacts with amino-containing molecules such as amino-modified oligos, proteins and peptides.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of QXY7 NHS ester [equivalent to QSY-7 NHS ester] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	126.371 µL	631.856 µL	1.264 mL	6.319 mL	12.637 mL
5 mM	25.274 µL	126.371 µL	252.742 µL	1.264 mL	2.527 mL
10 mM	12.637 µL	63.186 µL	126.371 µL	631.856 µL	1.264 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Absorbance (nm)	561
Correction Factor (280 nm)	0.22
Extinction coefficient (cm ^-1 M ^-1)	90000¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
XFD488 NHS Ester Same Structure to Alexa Fluor™ 488 NHS Ester	499	520	71000	0.92¹	0.30	0.11
XFD350 NHS Ester Same Structure to Alexa Fluor™ 350 NHS Ester	343	441	19000	-	0.25	0.19
XFD532 NHS Ester Same Structure to Alexa Fluor™ 532 NHS Ester	534	553	81000	0.61¹	0.24	0.09
XFD594 NHS Ester Same Structure to Alexa Fluor™ 594 NHS Ester	590	618	90000	0.66¹	0.43	0.56
QXY21 NHS ester [equivalent to QSY-21 NHS ester]	-	-	89000¹	-	-	0.32
XFD555 NHS Ester Same Structure to Alexa Fluor™ 555 NHS Ester	553	568	150000	0.1¹	0.08	0.08
XFD647 NHS Ester Same Structure to Alexa Fluor™ 647 NHS Ester	650	671	239000	0.33¹	0.00	0.03
XFD680 NHS Ester Same Structure to Alexa Fluor™ 680 NHS Ester	681	704	184000	0.36¹	0.00	0.05
XFD700 NHS Ester Same Structure to Alexa Fluor™ 700 NHS Ester	696	719	192000	0.25¹	0.00	0.07
XFD750 NHS Ester Same Structure to Alexa Fluor™ 750 NHS Ester	752	776	240000	0.12¹	0.00	0.04
Cy5DIGE NHS ester	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
Cy2DIGE NHS ester	492	508	150000	0.120	0.08	0.15
Cy3DIGE NHS ester	555	569	150000¹	0.15¹	0.07	0.073
XFD546 NHS Ester Same Structure to Alexa Fluor™ 546 NHS Ester	561	572	112000	0.79¹	0.21	0.12
XFD568 NHS Ester Same Structure to Alexa Fluor™ 568 NHS Ester	579	603	91300	0.69¹	0.45	0.46
XFD514 NHS Ester Same Structure to Alexa Fluor™ 514 NHS Ester	518	543	80000	-	0.31	0.18
Cy3B NHS ester	560	571	120000¹	0.58¹	0.048	0.069
CypHer5E NHS Ester	643	660	-	-	-	-
CypHer7E NHS Ester	748	769	-	-	-	-
Show More (10)

Images

Figure 1. Chemical structure for QXY7 NHS ester [equivalent to QSY-7 NHS ester].

References

View all 11 references: Citation Explorer

[Development of Novel Dark Quenchers and Their Application to Imaging Probes].
Authors: Hanaoka, Kenjiro
Journal: Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan (2019): 277-283

A real-time in vitro assay to evaluate the release of macromolecules from liposomes.
Authors: Mujoo, Himang and Reynolds, John N J and Tucker, Ian G
Journal: Drug testing and analysis (2018): 1025-1032

Structural exploration and Förster theory modeling for the interpretation of gas-phase FRET measurements: Chromophore-grafted amyloid-β peptides.
Authors: Kulesza, Alexander and Daly, Steven and MacAleese, Luke and Antoine, Rodolphe and Dugourd, Philippe
Journal: The Journal of chemical physics (2015): 025101

Action-FRET: probing the molecular conformation of mass-selected gas-phase peptides with Förster resonance energy transfer detected by acceptor-specific fragmentation.
Authors: Daly, Steven and Poussigue, Frédéric and Simon, Anne-Laure and MacAleese, Luke and Bertorelle, Franck and Chirot, Fabien and Antoine, Rodolphe and Dugourd, Philippe
Journal: Analytical chemistry (2014): 8798-804

Reversible off-on fluorescence probe for hypoxia and imaging of hypoxia-normoxia cycles in live cells.
Authors: Takahashi, Shodai and Piao, Wen and Matsumura, Yuriko and Komatsu, Toru and Ueno, Tasuku and Terai, Takuya and Kamachi, Toshiaki and Kohno, Masahiro and Nagano, Tetsuo and Hanaoka, Kenjiro
Journal: Journal of the American Chemical Society (2012): 19588-91

Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer.
Authors: Ogawa, Mikako and Kosaka, Nobuyuki and Choyke, Peter L and Kobayashi, Hisataka
Journal: Bioconjugate chemistry (2009): 147-54

Near-infrared fluorescent oligodeoxyribonucleotide reporters for sensing NF-kappaB DNA interactions in vitro.
Authors: Zhang, Surong and Metelev, Valeri and Tabatadze, David and Zamecnik, Paul and Bogdanov, Alexei
Journal: Oligonucleotides (2008): 235-43

Fluorescence-based sensing of glucose using engineered glucose/galactose-binding protein: a comparison of fluorescence resonance energy transfer and environmentally sensitive dye labelling strategies.
Authors: Khan, Faaizah and Gnudi, Luigi and Pickup, John C
Journal: Biochemical and biophysical research communications (2008): 102-6

A time-resolved, internally quenched fluorescence assay to characterize inhibition of hepatitis C virus nonstructural protein 3-4A protease at low enzyme concentrations.
Authors: Mao, Shi-Shan and DiMuzio, Jillian and McHale, Carolyn and Burlein, Christine and Olsen, David and Carroll, Steven S
Journal: Analytical biochemistry (2008): 1-8

Longer wavelength fluorescence resonance energy transfer depsipeptide substrates for hepatitis C virus NS3 protease.
Authors: Konstantinidis, Alex K and Richardson, Paul L and Kurtz, Kevin A and Tripathi, Rakesh and Chen, Chih-Ming and Huang, Peggy and Randolph, John and Towne, Danli and Donnelly, Jennifer and Warrior, Usha and Middleton, Tim and Kati, Warren M
Journal: Analytical biochemistry (2007): 156-67