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QXY7 NHS ester [equivalent to QSY-7 NHS ester]
QXY7 is the same molecule to QSY-7 acid (ThermoFisher). QXY7 has a broad and intense absorption at ~550 nm maxima with no detectable fluorescence, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications paring with Cy3, TAMRA, Alexa Fluor 555 and iFluor 647 or other spectrally similar fluorescent dyes. QXY7 NHS ester is quite stable and readily reacts with amino-containing molecules such as amino-modified oligos, proteins and peptides.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Cy2DIGE NHS ester | 492 | 508 | 150000 | 0.120 | 0.08 | 0.15 |
| Cy3B NHS ester | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
| Cy3DIGE NHS ester | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
| Cy5DIGE NHS ester | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
| CypHer5E NHS Ester | 643 | 660 | - | - | - | - |
| CypHer7E NHS Ester | 748 | 769 | - | - | - | - |
| QXY21 NHS ester [equivalent to QSY-21 NHS ester] | - | - | 890001 | - | - | 0.32 |
| XFD350 NHS Ester | 343 | 441 | 19000 | - | 0.25 | 0.19 |
| XFD430 NHS ester | 432 | 540 | 15,000 | - | - | 0.28 |
Show More (13) | ||||||
References
View all 11 references: Citation Explorer
[Development of Novel Dark Quenchers and Their Application to Imaging Probes].
Authors: Hanaoka, Kenjiro
Journal: Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan (2019): 277-283
Authors: Hanaoka, Kenjiro
Journal: Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan (2019): 277-283
A real-time in vitro assay to evaluate the release of macromolecules from liposomes.
Authors: Mujoo, Himang and Reynolds, John N J and Tucker, Ian G
Journal: Drug testing and analysis (2018): 1025-1032
Authors: Mujoo, Himang and Reynolds, John N J and Tucker, Ian G
Journal: Drug testing and analysis (2018): 1025-1032
Structural exploration and Förster theory modeling for the interpretation of gas-phase FRET measurements: Chromophore-grafted amyloid-β peptides.
Authors: Kulesza, Alexander and Daly, Steven and MacAleese, Luke and Antoine, Rodolphe and Dugourd, Philippe
Journal: The Journal of chemical physics (2015): 025101
Authors: Kulesza, Alexander and Daly, Steven and MacAleese, Luke and Antoine, Rodolphe and Dugourd, Philippe
Journal: The Journal of chemical physics (2015): 025101
Action-FRET: probing the molecular conformation of mass-selected gas-phase peptides with Förster resonance energy transfer detected by acceptor-specific fragmentation.
Authors: Daly, Steven and Poussigue, Frédéric and Simon, Anne-Laure and MacAleese, Luke and Bertorelle, Franck and Chirot, Fabien and Antoine, Rodolphe and Dugourd, Philippe
Journal: Analytical chemistry (2014): 8798-804
Authors: Daly, Steven and Poussigue, Frédéric and Simon, Anne-Laure and MacAleese, Luke and Bertorelle, Franck and Chirot, Fabien and Antoine, Rodolphe and Dugourd, Philippe
Journal: Analytical chemistry (2014): 8798-804
Reversible off-on fluorescence probe for hypoxia and imaging of hypoxia-normoxia cycles in live cells.
Authors: Takahashi, Shodai and Piao, Wen and Matsumura, Yuriko and Komatsu, Toru and Ueno, Tasuku and Terai, Takuya and Kamachi, Toshiaki and Kohno, Masahiro and Nagano, Tetsuo and Hanaoka, Kenjiro
Journal: Journal of the American Chemical Society (2012): 19588-91
Authors: Takahashi, Shodai and Piao, Wen and Matsumura, Yuriko and Komatsu, Toru and Ueno, Tasuku and Terai, Takuya and Kamachi, Toshiaki and Kohno, Masahiro and Nagano, Tetsuo and Hanaoka, Kenjiro
Journal: Journal of the American Chemical Society (2012): 19588-91
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