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CypHer7E NHS Ester

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Physical properties
Molecular weight864.91
Spectral properties
Excitation (nm)748
Emission (nm)769
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
CypHer7E NHS ester is an excellent building block for developing site-specific pH probes. It is analogous to CypHer5E NHS ester with much longer excitation and emission. It is one of the very few acidotropic pH probes that exhibit NIR fluorescence. It can be readily conjugated to amino-containing biomolecules (such as antibodies, peptides and amino-modified oligos). CypHer7E is a NIR pH-sensitive cyanine dye derivative that can be used to study most classes of cell surface receptors which internalize into the endocytic pathway when activated by a ligand. CypHer7E NHS ester is a flexible, generic fluorophore that can be coupled to a receptor or epitope tag antibody and monitors neutral to acidic intracellular events.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CypHer7E NHS Ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM115.619 µL578.095 µL1.156 mL5.781 mL11.562 mL
5 mM23.124 µL115.619 µL231.238 µL1.156 mL2.312 mL
10 mM11.562 µL57.809 µL115.619 µL578.095 µL1.156 mL

Molarity calculator

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Spectral properties

Excitation (nm)748
Emission (nm)769

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
XFD488 NHS Ester *Same Structure to Alexa Fluor™ 488 NHS Ester*499520710000.9210.300.11
XFD350 NHS Ester *Same Structure to Alexa Fluor™ 350 NHS Ester*34344119000-0.250.19
XFD532 NHS Ester *Same Structure to Alexa Fluor™ 532 NHS Ester*534553810000.6110.240.09
XFD594 NHS Ester *Same Structure to Alexa Fluor™ 594 NHS Ester*590618900000.6610.430.56
QXY21 NHS ester [equivalent to QSY-21 NHS ester]--890001--0.32
XFD555 NHS Ester *Same Structure to Alexa Fluor™ 555 NHS Ester*5535681500000.110.080.08
XFD647 NHS Ester *Same Structure to Alexa Fluor™ 647 NHS Ester*6506712390000.3310.000.03
XFD680 NHS Ester *Same Structure to Alexa Fluor™ 680 NHS Ester*6817041840000.3610.000.05
XFD700 NHS Ester *Same Structure to Alexa Fluor™ 700 NHS Ester*6967191920000.2510.000.07
XFD750 NHS Ester *Same Structure to Alexa Fluor™ 750 NHS Ester*7527762400000.1210.000.04
Cy5DIGE NHS ester65167025000010.271, 0.420.020.03
Cy2DIGE NHS ester4925081500000.1200.080.15
Cy3DIGE NHS ester55556915000010.1510.070.073
XFD546 NHS Ester *Same Structure to Alexa Fluor™ 546 NHS Ester*5615721120000.7910.210.12
XFD568 NHS Ester *Same Structure to Alexa Fluor™ 568 NHS Ester*579603913000.6910.450.46
XFD514 NHS Ester *Same Structure to Alexa Fluor™ 514 NHS Ester*51854380000-0.310.18
QXY7 NHS ester [equivalent to QSY-7 NHS ester]--900001--0.22
Cy3B NHS ester56057112000010.5810.0480.069
CypHer5E NHS Ester643660----
XFD660 NHS Ester [equivalent to Alexa Fluor® 660 NHS Ester]663691132000---
Show More (11)



View all 15 references: Citation Explorer
Altered exocytosis of inhibitory synaptic vesicles at single presynaptic terminals of cultured striatal neurons in a knock-in mouse model of Huntington's disease.
Authors: Xu, Chen and Chen, Sidong and Chen, Xingxiang and Ho, Ka Hei and Park, Chungwon and Yoo, Hanna and Lee, Suk-Ho and Park, Hyokeun
Journal: Frontiers in molecular neuroscience (2023): 1175522
Measuring Phagosomal pH by Fluorescence Microscopy.
Authors: Gonzales, Gerone A and Canton, Johnathan
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 153-169
A near-infrared fluorescent pH sensing film for wound milieu pH monitoring.
Authors: Li, Shuxin and Vu, Hong and Senkowsky, Jon and Hu, Wenjing and Tang, Liping
Journal: Experimental dermatology (2020): 107-111
RNAi in Spodoptera frugiperda Sf9 Cells via Nanomaterial Mediated Delivery of dsRNA: A Comparison of Poly-l-arginine Polyplexes and Poly-l-arginine-Functionalized Au Nanoparticles.
Authors: Laisney, Jérôme and Gurusamy, Dhandapani and Baddar, Zeinah Elhaj and Palli, Subba Reddy and Unrine, Jason M
Journal: ACS applied materials & interfaces (2020): 25645-25657
Transport of orally delivered dsRNA in southern green stink bug, Nezara viridula.
Authors: Gurusamy, Dhandapani and Howell, Jeffrey L and Chereddy, Shankar C R R and Koo, Jinmo and Palli, Subba Reddy
Journal: Archives of insect biochemistry and physiology (2020): e21692
Distinct modes of endocytotic presynaptic membrane and protein uptake at the calyx of Held terminal of rats and mice.
Authors: Okamoto, Yuji and Lipstein, Noa and Hua, Yunfeng and Lin, Kun-Han and Brose, Nils and Sakaba, Takeshi and Midorikawa, Mitsuharu
Journal: eLife (2016)
High-Throughput Screening for Internalizing Antibodies by Homogeneous Fluorescence Imaging of a pH-Activated Probe.
Authors: Riedl, Thilo and van Boxtel, Egon and Bosch, Martijn and Parren, Paul W H I and Gerritsen, Arnout F
Journal: Journal of biomolecular screening (2016): 12-23
The antidepressant fluoxetine mobilizes vesicles to the recycling pool of rat hippocampal synapses during high activity.
Authors: Jung, Jasmin and Loy, Kristina and Schilling, Eva-Maria and Röther, Mareike and Brauner, Jan M and Huth, Tobias and Schlötzer-Schrehardt, Ursula and Alzheimer, Christian and Kornhuber, Johannes and Welzel, Oliver and Groemer, Teja W
Journal: Molecular neurobiology (2014): 916-30
Real-time noninvasive monitoring of in vivo inflammatory responses using a pH ratiometric fluorescence imaging probe.
Authors: Tsai, Yi-Ting and Zhou, Jun and Weng, Hong and Shen, Jinhui and Tang, Liping and Hu, Wen-Jing
Journal: Advanced healthcare materials (2014): 221-9
The pH probe CypHer™5E is effectively quenched by FM dyes.
Authors: Welzel, Oliver and Loy, Kristina and Tischbirek, Carsten H and Tabor, Alina and Gmeiner, Peter and Kornhuber, Johannes and Groemer, Teja W
Journal: Journal of fluorescence (2013): 487-94