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XFD405 NHS Ester [equivalent to Alexa Fluor™ 405 NHS Ester]
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1028.26 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Related products
| Overview |  SDSProtocol |
See also: Antibody and Protein Labeling
Molecular weight 1028.26 |
XFD405 NHS Ester (Succinimidyl Ester) is the same molecule as Alexa Fluor® 405 NHS Ester (#A30000 from ThermoFisher, Alexa Fluor® is the trademark of ThermoFisher). It is a moderately bright blue-fluorescent dye optimal for use with the 405 nm Violet laser. XFD405 dye is water soluble and pH-insensitive from pH 4 to pH 10. XFD405 NHS ester is the most convenient amine-reactive form for conjugating this dye to a protein or antibody. XFD405 NHS Ester is primarily used for preparing fluorescent antibody conjugates for multicolor flow cytometry applications.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of XFD405 NHS Ester [equivalent to Alexa Fluor™ 405 NHS Ester] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 97.252 µL | 486.258 µL | 972.517 µL | 4.863 mL | 9.725 mL |
| 5 mM | 19.45 µL | 97.252 µL | 194.503 µL | 972.517 µL | 1.945 mL |
| 10 mM | 9.725 µL | 48.626 µL | 97.252 µL | 486.258 µL | 972.517 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| XFD488 NHS Ester *Same Structure to Alexa Fluor™ 488 NHS Ester* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
| XFD350 NHS Ester *Same Structure to Alexa Fluor™ 350 NHS Ester* | 343 | 441 | 19000 | - | 0.25 | 0.19 |
| XFD532 NHS Ester *Same Structure to Alexa Fluor™ 532 NHS Ester* | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
| XFD594 NHS Ester *Same Structure to Alexa Fluor™ 594 NHS Ester* | 590 | 618 | 90000 | 0.661 | 0.43 | 0.56 |
| XFD555 NHS Ester *Same Structure to Alexa Fluor™ 555 NHS Ester* | 553 | 568 | 150000 | 0.11 | 0.08 | 0.08 |
| XFD647 NHS Ester *Same Structure to Alexa Fluor™ 647 NHS Ester* | 650 | 671 | 239000 | 0.331 | 0.00 | 0.03 |
| XFD680 NHS Ester *Same Structure to Alexa Fluor™ 680 NHS Ester* | 681 | 704 | 184000 | 0.361 | 0.00 | 0.05 |
| XFD700 NHS Ester *Same Structure to Alexa Fluor™ 700 NHS Ester* | 696 | 719 | 192000 | 0.251 | 0.00 | 0.07 |
| XFD750 NHS Ester *Same Structure to Alexa Fluor™ 750 NHS Ester* | 752 | 776 | 240000 | 0.121 | 0.00 | 0.04 |
Show More (11) | ||||||
Images
Figure 1. XFD405 NHS Ester (Succinimidyl Ester) is the same molecule as Alexa Fluor® 405 NHS Ester (#A30000 from ThermoFisher, Alexa Fluor® is the trademark of ThermoFisher). It is primarily used for preparing fluorescent antibody conjugates for multicolor flow cytometry applications.
References
View all 6 references: Citation Explorer
Effects of Viscosity and Refractive Index on the Emission and Diffusion Properties of Alexa Fluor 405 Using Fluorescence Correlation and Lifetime Spectroscopies.
Authors: van Zanten, Camila and Melnikau, Dzmitry and Ryder, Alan G
Journal: Journal of fluorescence (2021): 835-845
Authors: van Zanten, Camila and Melnikau, Dzmitry and Ryder, Alan G
Journal: Journal of fluorescence (2021): 835-845
Author Correction: A dynamic three-step mechanism drives the HIV-1 pre-fusion reaction.
Authors: Iliopoulou, Maro and Nolan, Rory and Alvarez, Luis and Watanabe, Yasunori and Coomer, Charles A and Jakobsdottir, G Maria and Bowden, Thomas A and Padilla-Parra, Sergi
Journal: Nature structural & molecular biology (2019): 526
Authors: Iliopoulou, Maro and Nolan, Rory and Alvarez, Luis and Watanabe, Yasunori and Coomer, Charles A and Jakobsdottir, G Maria and Bowden, Thomas A and Padilla-Parra, Sergi
Journal: Nature structural & molecular biology (2019): 526
Egg autofluorescence and options for detecting peanut agglutinin binding for the identification of Haemonchus contortus eggs in fecal samples.
Authors: Abbas, Ibrahim and Hildreth, Michael
Journal: Veterinary parasitology (2019): 69-74
Authors: Abbas, Ibrahim and Hildreth, Michael
Journal: Veterinary parasitology (2019): 69-74
Identification of novel cell-impermeant fluorescent substrates for testing the function and drug interaction of Organic Anion-Transporting Polypeptides, OATP1B1/1B3 and 2B1.
Authors: Patik, Izabel and Székely, Virág and Német, Orsolya and Szepesi, Áron and Kucsma, Nóra and Várady, György and Szakács, Gergely and Bakos, Éva and Özvegy-Laczka, Csilla
Journal: Scientific reports (2018): 2630
Authors: Patik, Izabel and Székely, Virág and Német, Orsolya and Szepesi, Áron and Kucsma, Nóra and Várady, György and Szakács, Gergely and Bakos, Éva and Özvegy-Laczka, Csilla
Journal: Scientific reports (2018): 2630
Energy transfer between a biological labelling dye and gold nanorods.
Authors: Racknor, Chris and Singh, Mahi R and Zhang, Yinan and Birch, David J S and Chen, Yu
Journal: Methods and applications in fluorescence (2013): 015002
Authors: Racknor, Chris and Singh, Mahi R and Zhang, Yinan and Birch, David J S and Chen, Yu
Journal: Methods and applications in fluorescence (2013): 015002
Violet laser diodes in flow cytometry: an update.
Authors: Telford, William and Kapoor, Veena and Jackson, James and Burgess, Walter and Buller, Gayle and Hawley, Teresa and Hawley, Robert
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2006): 1153-60
Authors: Telford, William and Kapoor, Veena and Jackson, James and Burgess, Walter and Buller, Gayle and Hawley, Teresa and Hawley, Robert
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2006): 1153-60
Application notes
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A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
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Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
FAQ
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