Name: ReadiUse™ Preactivated PerCP
Brand: AAT Bioquest
SKU: 2590
Price: 339 USD
Availability: InStock

ReadiUse™ Preactivated PerCP

ReadiUse™ Preactivated PerCP is a ready-to-conjugate fluorescent protein label for preparing antibody and protein conjugates used in flow cytometry.

Fluorescence Detection: Excitation at 488 nm and emission at 678 nm.
Conjugation Chemistry: Buccutite™ FOL/MTA chemistry enables protein conjugation through amino-group modification.
High Conjugation Yield: Higher yields than conventional SMCC-based chemistry.
Ready-to-Use: Includes preactivated PerCP and Buccutite™ MTA reagent.
Applications: Suitable for fluorescent immunolabeling and flow cytometry.

Conjugation scheme for ReadiUse™ Preactivated PerCP. The activated PerCP label is premodified with our Buccutite™ FOL and can be readily used for conjugation. To conjugate your desired antibody or protein, first modify it with our Buccutite™ MTA (provided) and then mix both components. The Buccutite™ MTA-modified protein will readily react with the Buccutite™ FOL-activated PerCP to give the desired PerCP-antibody conjugate in much higher yield than SMCC chemistry. In addition our preactivated PerCP reacts with MTA-modified biopolymers at a much lower concentration than SMCC chemistry.

Protocol

SDS

Catalog	Size	Price	Quantity
2590	1 mg	Price

Overview

PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. It has an extremely high extinction coefficient, a high quantum efficiency and a large Stokes shift. It is well excited with the Argon laser at 488 nm with its maximum emission peak at 677 nm. PerCP protein is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting (FACS). Its tandem conjugates (such as PerCP-Cy5.5) can be excited with a standard 488 nm laser and emits in the far red at a longer wavelength for multicolor flow cytometric analysis of cells. These multiple emission wavelengths make PerCP- Cyanine conjugates potentially useful fluorochromes for multicolor analysis with FITC, PE and other fluorochromes. PerCP tandem structure may make it more photostable than PerCP alone, which generally photobleaches rapidly with more powerful water-cooled gas lasers. AAT Bioquest offers this preactivated PerCP to facilitate the PerCP conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PerCP is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated PerCP is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.

References

View all 50 references: Citation Explorer

Chronic and oxidative stress association with total count of endothelial microvesicles in healthy young male plasma.

Authors:

Žėkas, Vytautas and Matuzevičienė, Reda and Karčiauskaitė, Dovilė and Mažeikienė, Asta and Burokienė, Neringa and Radzevičius, Mantas and Janilionienė, Aušra and Linkevičiūtė, Aušra and Kučinskienė, Zita Aušrelė

Journal:

Advances in clinical and experimental medicine : official organ Wroclaw Medical University (2019): 683-692

B lymphocyte subsets and outcomes in patients with an initial diagnosis of transient hypogammaglobulinemia of infancy.

Authors:

Eroglu, Fehime K and Aerts Kaya, Fatima and Cagdas, Deniz and Özgür, Tuba Turul and Yılmaz, Togay and Tezcan, İlhan and Sanal, Özden

Journal:

Scandinavian journal of immunology (2018): e12709

Exogenous IL-9 Ameliorates Experimental Autoimmune Myasthenia Gravis Symptoms in Rats.

Authors:

Yao, Xiuhua and Zhao, Jiarui and Kong, Qingfei and Xie, Xiaoli and Wang, Jinghua and Sun, Bo and Xu, Lixia and Mu, Lili and Li, Hulun

Journal:

Immunological investigations (2018): 712-724

One tube with eight antibodies for 14-part bone marrow leukocyte differential using flow cytometry.

Authors:

Jacob, Marie-Christine and Souvignet, Alice and Pont, Julie and Solly, Françoise and Mondet, Julie and Kesr, Sanae and Pernollet, Martine and Dumestre-Perard, Chantal and Campos, Lydia and Cesbron, Jean-Yves

Journal:

Cytometry. Part B, Clinical cytometry (2017): 299-309

[Diagnostic Value of CD27 Antigen in Patients with Multiple Myeloma].

Authors:

Wang, Hui and Liu, Lu and Liu, Fang and Chen, Li-Juan and Qu, Xiao-Yan and Li, Jian-Yong and Wu, Yu-Jie

Journal:

Zhongguo shi yan xue ye xue za zhi (2017): 1069-1073

Spectral properties

Correction factor (280 nm)	0.22
Extinction coefficient (cm ^-1 M ^-1)	406000
Excitation (nm)	477
Emission (nm)	678

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on October 15, 2026

A: ReadiUse™ Preactivated PerCP	1 vial (1 mg)
B: Buccutite™ MTA	1 vial (100 µg)
C: Spin Desalting Column	Not Included