AAT Bioquest

ReadiUse™ Preactivated PerCP-Cy7 Maleimide

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Physical properties
Molecular weightN/A
Spectral properties
Extinction coefficient (cm -1 M -1)350000
Excitation (nm)757
Emission (nm)783
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
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See also: PE and APC
Molecular weight
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
PerCP-Cy7 is a popular tandem color used in flow cytometry. Its primary absorption peak is at ~480 nm with emission peak at ~780 nm. AAT Bioquest offers this preactivated PerCP-Cy7 to facilitate the PerCP-Cy7 tandem conjugations to reduced antibodies and other biomolecules that contain a thiol group. Our preactivated PerCP-Cy7 maleimide is prepared from the commonly used crosslinker SMCC, and ready to conjugate. PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. It has an extremely high extinction coefficient, a high quantum efficiency and a large Stokes shift. Its tandem conjugates (such as PerCP-Cy7) can be excited with a standard 488 nm laser and emits in the far red at a longer wavelength for multicolor flow cytometric analysis of cells. These multiple emission wavelengths make PerCP- Cyanine conjugates potentially useful fluorochromes for multicolor analysis with FITC, PE and other fluorochromes. PerCP tandem structure may make it more photostable than PerCP alone, which generally photobleaches rapidly with more powerful water-cooled gas lasers.


Open in Advanced Spectrum Viewer

Spectral properties

Extinction coefficient (cm -1 M -1)350000
Excitation (nm)757
Emission (nm)783

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
ReadiUse™ Preactivated PE-Cy7 Maleimide5657781960000
ReadiUse™ Preactivated APC-Cy7 Maleimide651779700000


View all 15 references: Citation Explorer
Routine flow cytometry approach for the evaluation of solid tumor neoplasms and immune cells in minimally invasive samples.
Authors: Quirós-Caso, Covadonga and Arias Fernández, Tamara and Fonseca-Mourelle, Ariana and Torres, Héctor and Fernández, Luis and Moreno-Rodríguez, Maria and Ariza-Prota, Miguel Ángel and López-González, Francisco Julián and Carvajal-Álvarez, Miguel and Alonso-Álvarez, Sara and Moro-García, Marco Antonio and Colado, Enrique
Journal: Cytometry. Part B, Clinical cytometry (2022)
Recombinant thrombomodulin attenuates hyper-inflammation and glycocalyx damage in a murine model of Streptococcus pneumoniae-induced sepsis.
Authors: Watanabe, Eizo and Akamatsu, Toshinobu and Ohmori, Masaaki and Kato, Mayu and Takeuchi, Noriko and Ishiwada, Naruhiko and Nishimura, Rintaro and Hishiki, Haruka and Fujimura, Lisa and Ito, Chizuru and Hatano, Masahiko
Journal: Cytokine (2022): 155723
The ISCCA flow protocol for the monitoring of anti-CD20 therapies in autoimmune disorders.
Authors: Gatti, Arianna and Buccisano, Francesco and Scupoli, Maria T and Brando, Bruno
Journal: Cytometry. Part B, Clinical cytometry (2021): 194-205
[Clinical features of imbalance between Th1 and Th22 cells and its association with disease progression in patients with liver cirrhosis].
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Journal: Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology (2017): 738-744
Authors: Kudryavtsev, I V and Serebryakova, M K and Totolyan, A A
Journal: Klinicheskaia laboratornaia diagnostika (2016): 179-84
Inhibition of histone deacetylase 6 restores innate immune cells in the bone marrow in a lethal septic model.
Authors: Zhao, Ting and Li, Yongqing and Liu, Baoling and Pan, Baihong and Cheng, Xin and Georgoff, Patrick and Alam, Hasan B
Journal: The journal of trauma and acute care surgery (2016): 34-40; discussion 40-1
Flow cytometric analysis of T lymphocyte proliferation in vivo by EdU incorporation.
Authors: Sun, Xiaojing and Zhang, Chunpan and Jin, Hua and Sun, Guangyong and Tian, Yue and Shi, Wen and Zhang, Dong
Journal: International immunopharmacology (2016): 56-65
Using six-colour flow cytometry to analyse the activation and interaction of platelets and leukocytes--A new assay suitable for bench and bedside conditions.
Authors: Granja, Tiago and Schad, Jessica and Schüssel, Patricia and Fischer, Claudius and Häberle, Helene and Rosenberger, Peter and Straub, Andreas
Journal: Thrombosis research (2015): 786-96
Presence of CD34(+)CD38(-)CD58(-) leukemia-propagating cells at diagnosis identifies patients at high risk of relapse with Ph chromosome-positive ALL after allo-hematopoietic SCT.
Authors: Kong, Y and Xu, L-P and Liu, Y-R and Qin, Y-Z and Sun, Y-Q and Wang, Y and Jiang, H and Jiang, Q and Chen, H and Chang, Y-J and Huang, X-J
Journal: Bone marrow transplantation (2015): 348-53
Combined normal donor and CLL: Single tube ZAP-70 analysis.
Authors: Degheidy, Heba A and Venzon, David J and Farooqui, Mohammed Z H and Abbasi, Fatima and Arthur, Diane C and Wilson, Wyndham H and Wiestner, Adrian and Stetler-Stevenson, M A and Marti, Gerald E
Journal: Cytometry. Part B, Clinical cytometry (2012): 67-77