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ReadiUse™ Preactivated PerCP-Cy7 Maleimide

Ordering information
Price ()
Catalog Number2725
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weightN/A
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
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OverviewpdfSDSpdfProtocol


Molecular weight
N/A
PerCP-Cy7 is a popular tandem color used in flow cytometry. Its primary absorption peak is at ~480 nm with emission peak at ~780 nm. AAT Bioquest offers this preactivated PerCP-Cy7 to facilitate the PerCP-Cy7 tandem conjugations to reduced antibodies and other biomolecules that contain a thiol group. Our preactivated PerCP-Cy7 maleimide is prepared from the commonly used crosslinker SMCC, and ready to conjugate. PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. It has an extremely high extinction coefficient, a high quantum efficiency and a large Stokes shift. Its tandem conjugates (such as PerCP-Cy7) can be excited with a standard 488 nm laser and emits in the far red at a longer wavelength for multicolor flow cytometric analysis of cells. These multiple emission wavelengths make PerCP- Cyanine conjugates potentially useful fluorochromes for multicolor analysis with FITC, PE and other fluorochromes. PerCP tandem structure may make it more photostable than PerCP alone, which generally photobleaches rapidly with more powerful water-cooled gas lasers.

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
ReadiUse™ Preactivated PE-Cy7 Maleimide5667781960000
ReadiUse™ Preactivated APC-Cy7 Maleimide754779700000

References


View all 15 references: Citation Explorer
Recombinant thrombomodulin attenuates hyper-inflammation and glycocalyx damage in a murine model of Streptococcus pneumoniae-induced sepsis.
Authors: Watanabe, Eizo and Akamatsu, Toshinobu and Ohmori, Masaaki and Kato, Mayu and Takeuchi, Noriko and Ishiwada, Naruhiko and Nishimura, Rintaro and Hishiki, Haruka and Fujimura, Lisa and Ito, Chizuru and Hatano, Masahiko
Journal: Cytokine (2022): 155723
Routine flow cytometry approach for the evaluation of solid tumor neoplasms and immune cells in minimally invasive samples.
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Journal: Cytometry. Part B, Clinical cytometry (2022)
The ISCCA flow protocol for the monitoring of anti-CD20 therapies in autoimmune disorders.
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Journal: International immunopharmacology (2016): 56-65
Inhibition of histone deacetylase 6 restores innate immune cells in the bone marrow in a lethal septic model.
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