Tetramethylrhodamine-dUTP 1 mM in Tris Buffer (pH 7.5)

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Physical properties

Molecular weight	1115.77
Solvent	Water

Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	552
Emission (nm)	578

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

Overview SDS Protocol

Molecular weight

1115.77

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.178

Extinction coefficient (cm ^-1 M ^-1)

90000

Excitation (nm)

552

Emission (nm)

578

The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This TMR-dUTP conjugate can be used as an orange fluorescence color with Spectrum Orange™ filter set (Spectrum Orange™ is the trademark of Vysis).

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Tetramethylrhodamine-dUTP *1 mM in Tris Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	89.624 µL	448.121 µL	896.242 µL	4.481 mL	8.962 mL
5 mM	17.925 µL	89.624 µL	179.248 µL	896.242 µL	1.792 mL
10 mM	8.962 µL	44.812 µL	89.624 µL	448.121 µL	896.242 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	552
Emission (nm)	578

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
TF1-dUTP 1 mM in Tris Buffer (pH 7.5)	341	448	20000	0.187
TF2-dUTP 1 mM in Tris Buffer (pH 7.5)	503	525	75000	0.09
TF3-dUTP 1 mM in Tris Buffer (pH 7.5)	554	578	75000¹	0.179
TF4-dUTP 1 mM in Tris Buffer (pH 7.5)	578	602	90000	0.436
TF5-dUTP 1 mM in Tris Buffer (pH 7.5)	649	664	250000	0.027
DEAC-dUTP 1 mM in Tris Buffer (pH 7.5)	411	472	-	0.12

Images

Figure 1. Chemical structure for Tetramethylrhodamine-dUTP *1 mM in Tris Buffer (pH 7.5)*

References

View all 9 references: Citation Explorer

Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114

A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685

Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919

Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518

Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405

Quantitative analysis of polymerase chain reaction products using biotinylated dUTP incorporation
Authors: Duplaa C, Couffinhal T, Labat L, Moreau C, Lamaziere JM, Bonnet J.
Journal: Anal Biochem (1993): 229

A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates
Authors: Urabe T, Sano K, Tanno M, Mizoguchi J, Otani M, Lee MH, Takasaki T, Kusakabe H, Imagawa DT, Nakai M.
Journal: J Virol Methods (1992): 145

Affinity isolation of active murine erythroleukemia cell chromatin: uniform distribution of ubiquitinated histone H2A between active and inactive fractions
Authors: Dawson BA, Herman T, Haas AL, Lough J.
Journal: J Cell Biochem (1991): 166

Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2'-deoxyuridine-5'-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin)
Authors: Muhlegger K, Huber E, von der Eltz H, Ruger R, Kessler C.
Journal: Biol Chem Hoppe Seyler (1990): 953