Tide Quencher™ 8WS acid [TQ8WS acid]
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 877.90 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
Tide Quencher™ 8WS amine [TQ8WS amine] |
Tide Quencher™ 8WS maleimide [TQ8WS maleimide] |
Tide Quencher™ 8WS azide [TQ8WS azide] |
Tide Quencher™ 8WS alkyne [TQ8WS alkyne] |
Related products
Overview | ![]() ![]() |
See also: Tide Quencher Dyes
Molecular weight 877.90 |
Tide Quencher™ 8WS (TQ8WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of IR fluorophores such as ICG, iFluor® 820, iFluor® 840 and iFluor® 860. It has the longest absorption wavelength among all the commercial quenchers. TQ8WS is designed to be the most effective IR quencher with (a). much stronger absorption, and (b). much higher quenching efficiency for IR dyes. Tide Quencher™ 8WS acid is primarily used for labeling amino-modified oligonucleotides and peptides. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ8WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ8WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively "quenching" the fluorescence signal.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 8WS acid [TQ8WS acid] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 113.908 µL | 569.541 µL | 1.139 mL | 5.695 mL | 11.391 mL |
5 mM | 22.782 µL | 113.908 µL | 227.816 µL | 1.139 mL | 2.278 mL |
10 mM | 11.391 µL | 56.954 µL | 113.908 µL | 569.541 µL | 1.139 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Product Family
Name | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
Tide Quencher™ 2WS acid [TQ2WS acid] | 48000 | 0.100 | 0.120 |
Tide Quencher™ 4WS acid [TQ4WS acid] | 900001 | 0.149 | 0.136 |
Tide Quencher™ 5WS acid [TQ5WS acid] | 130000 | 0.072 | 0.082 |
Tide Quencher™ 6WS acid [TQ6WS acid] | 130000 | 0.120 | 0.102 |
Tide Quencher™ 7WS acid [TQ7WS acid] | 140000 | 0.072 | 0.091 |
Tide Quencher™ 1 acid [TQ1 acid] | 20000 | 0.147 | 0.194 |
Tide Quencher™ 2 acid [TQ2 acid] | 21000 | 0.100 | 0.12 |
Tide Quencher™ 3 acid [TQ3 acid] | 22000 | 0.085 | 0.091 |
Tide Quencher™ 3WS acid [TQ3WS acid] | 90000 | 0.186 | 0.205 |
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Images
References
View all 50 references: Citation Explorer
Specific Targeting and Labeling of Colonic Polyps in CPC-APC Mice with Mucin 5AC Fluorescent Antibodies: A Model for Detection of Early Colon Cancer.
Authors: Turner, Michael A and Cox, Kristin E and Liu, Shanglei and Neel, Nicholas and Amirfakhri, Siamak and Nishino, Hiroto and Hosseini, Mojgan and Alcantara, Joshua A and Abd El-Hafeez, Amer Ali and Lwin, Thinzar M and Mallya, Kavita and Pisegna, Joseph R and Singh, Satish K and Ghosh, Pradipta and Hoffman, Robert M and Batra, Surinder K and Bouvet, Michael
Journal: Current issues in molecular biology (2023): 3347-3358
Authors: Turner, Michael A and Cox, Kristin E and Liu, Shanglei and Neel, Nicholas and Amirfakhri, Siamak and Nishino, Hiroto and Hosseini, Mojgan and Alcantara, Joshua A and Abd El-Hafeez, Amer Ali and Lwin, Thinzar M and Mallya, Kavita and Pisegna, Joseph R and Singh, Satish K and Ghosh, Pradipta and Hoffman, Robert M and Batra, Surinder K and Bouvet, Michael
Journal: Current issues in molecular biology (2023): 3347-3358
Performance of two clinical fluorescence imaging systems with different targeted and non-targeted near-infrared fluorophores: a cadaveric explorative study.
Authors: Chiti, Lavinia E and Husi, Benjamin and Park, Brian and Beer, Patricia and D'Orchymont, Faustine and Holland, Jason P and Nolff, Mirja C
Journal: Frontiers in veterinary science (2023): 1091842
Authors: Chiti, Lavinia E and Husi, Benjamin and Park, Brian and Beer, Patricia and D'Orchymont, Faustine and Holland, Jason P and Nolff, Mirja C
Journal: Frontiers in veterinary science (2023): 1091842
Optimizing Axial and Peripheral Substitutions in Si-Centered Naphthalocyanine Dyes for Enhancing Aqueous Solubility and Photoacoustic Signal Intensity.
Authors: Saad, Mohammad Ahsan and Pawle, Robert and Selfridge, Scott and Contreras, Leslie and Xavierselvan, Marvin and Nguyen, Christopher D and Mallidi, Srivalleesha and Hasan, Tayyaba
Journal: International journal of molecular sciences (2023)
Authors: Saad, Mohammad Ahsan and Pawle, Robert and Selfridge, Scott and Contreras, Leslie and Xavierselvan, Marvin and Nguyen, Christopher D and Mallidi, Srivalleesha and Hasan, Tayyaba
Journal: International journal of molecular sciences (2023)
Preclinical assessment of IRDye800CW-labeled gastrin-releasing peptide receptor-targeting peptide for near infrared-II imaging of brain malignancies.
Authors: Zhang, Yuan and Wang, Li and Zhang, Chengkai and Zhang, Jingjing and Yuan, Linhao and Jin, Shucheng and Zhou, Wenjianlong and Guan, Xiudong and Kang, Peng and Zhang, Chuanbao and Tian, Jie and Chen, Xiaoyuan and Li, Deling and Jia, Wang
Journal: Bioengineering & translational medicine (2023): e10532
Authors: Zhang, Yuan and Wang, Li and Zhang, Chengkai and Zhang, Jingjing and Yuan, Linhao and Jin, Shucheng and Zhou, Wenjianlong and Guan, Xiudong and Kang, Peng and Zhang, Chuanbao and Tian, Jie and Chen, Xiaoyuan and Li, Deling and Jia, Wang
Journal: Bioengineering & translational medicine (2023): e10532
Enhancing intraoperative tumor delineation with multispectral short-wave infrared fluorescence imaging and machine learning.
Authors: Waterhouse, Dale J and Privitera, Laura and Anderson, John and Stoyanov, Danail and Giuliani, Stefano
Journal: Journal of biomedical optics (2023): 094804
Authors: Waterhouse, Dale J and Privitera, Laura and Anderson, John and Stoyanov, Danail and Giuliani, Stefano
Journal: Journal of biomedical optics (2023): 094804
Impact of Surgical Lights on the Performance of Fluorescence-Guided Surgery Systems: A Pilot Study.
Authors: Chiti, Lavinia E and Park, Brian and d'Orchymont, Faustine and Holland, Jason P and Nolff, Mirja C
Journal: Animals : an open access journal from MDPI (2023)
Authors: Chiti, Lavinia E and Park, Brian and d'Orchymont, Faustine and Holland, Jason P and Nolff, Mirja C
Journal: Animals : an open access journal from MDPI (2023)
Test method for evaluating the photocytotoxic potential of fluorescence imaging products.
Authors: Vig, Shruti and Gaitan, Brandon and Frankle, Lucas and Chen, Yu and Elespuru, Rosalie and Pfefer, T Joshua and Huang, Huang-Chiao
Journal: Photochemistry and photobiology (2023)
Authors: Vig, Shruti and Gaitan, Brandon and Frankle, Lucas and Chen, Yu and Elespuru, Rosalie and Pfefer, T Joshua and Huang, Huang-Chiao
Journal: Photochemistry and photobiology (2023)
The Use of Panitumumab-IRDye800CW in a Novel Murine Model for Conjunctival Squamous Cell Carcinoma.
Authors: Youn, Gun Min and Case, Ayden G and Jarin, Trent and Li, BaoXiang and Swarup, Aditi and Naranjo, Andrea and Bou-Khalil, Charbel and Yao, Jacqueline and Zhou, Quan and Hom, Marisa E and Rosenthal, Eben L and Wu, Albert Y
Journal: Translational vision science & technology (2022): 23
Authors: Youn, Gun Min and Case, Ayden G and Jarin, Trent and Li, BaoXiang and Swarup, Aditi and Naranjo, Andrea and Bou-Khalil, Charbel and Yao, Jacqueline and Zhou, Quan and Hom, Marisa E and Rosenthal, Eben L and Wu, Albert Y
Journal: Translational vision science & technology (2022): 23
89Zr-panitumumab Combined With 18F-FDG PET Improves Detection and Staging of Head and Neck Squamous Cell Carcinoma.
Authors: Lee, Yu-Jin and van den Berg, Nynke S and Duan, Heying and Azevedo, E Carmen and Ferri, Valentina and Hom, Marisa and Raymundo, Roan C and Valencia, Alex and Castillo, Jessa and Shen, Bin and Zhou, Quan and Freeman, Laura and Koran, Mary Ellen and Kaplan, Michael J and Colevas, A Dimitrios and Baik, Fred M and Chin, Frederick T and Martin, Brock A and Iagaru, Andrei and Rosenthal, Eben L
Journal: Clinical cancer research : an official journal of the American Association for Cancer Research (2022): 4425-4434
Authors: Lee, Yu-Jin and van den Berg, Nynke S and Duan, Heying and Azevedo, E Carmen and Ferri, Valentina and Hom, Marisa and Raymundo, Roan C and Valencia, Alex and Castillo, Jessa and Shen, Bin and Zhou, Quan and Freeman, Laura and Koran, Mary Ellen and Kaplan, Michael J and Colevas, A Dimitrios and Baik, Fred M and Chin, Frederick T and Martin, Brock A and Iagaru, Andrei and Rosenthal, Eben L
Journal: Clinical cancer research : an official journal of the American Association for Cancer Research (2022): 4425-4434
Towards Photodynamic Image-Guided Surgery of Head and Neck Tumors: Photodynamic Priming Improves Delivery and Diagnostic Accuracy of Cetuximab-IRDye800CW.
Authors: Bhandari, Chanda and Fakhry, John and Eroy, Menitte and Song, Jane Junghwa and Samkoe, Kimberley and Hasan, Tayyaba and Hoyt, Kenneth and Obaid, Girgis
Journal: Frontiers in oncology (2022): 853660
Authors: Bhandari, Chanda and Fakhry, John and Eroy, Menitte and Song, Jane Junghwa and Samkoe, Kimberley and Hasan, Tayyaba and Hoyt, Kenneth and Obaid, Girgis
Journal: Frontiers in oncology (2022): 853660