XFD™488-dUTP *1 mM in Tris Buffer (pH 7.5)*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1152.83 |
Solvent | Water |
Spectral properties
Correction Factor (260 nm) | 0.3 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 73000 |
Excitation (nm) | 499 |
Emission (nm) | 520 |
Quantum yield | 0.921 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | SDSProtocol |
See also: Antibody and Protein Labeling, Fluorescent in situ hybridization (FISH), Nucleic Acid Building Blocks, RNA Purification & Analysis
Molecular weight 1152.83 | Correction Factor (260 nm) 0.3 | Correction Factor (280 nm) 0.11 | Extinction coefficient (cm -1 M -1) 73000 | Excitation (nm) 499 | Emission (nm) 520 | Quantum yield 0.921 |
The dye-modified deoxyuridine 5'-triphosphates are one of the most common methods to produce dye-labelled DNA via the conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This XFD 488-dUTP conjugate has the same chemical structure to ChromaTide™Alexa Fluor 488-5-dUTP. It is used as a green fluorescence color with SpectrumGreen™ filter set. It provides a bright signal that has high photostability and is not affected by pH. ChromaTide™ and Alexa Fluor™ are the trademarks of ThermoFisher, and SpectrumGreen™ is the trademark of Vysis.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of XFD™488-dUTP *1 mM in Tris Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 86.743 µL | 433.715 µL | 867.431 µL | 4.337 mL | 8.674 mL |
5 mM | 17.349 µL | 86.743 µL | 173.486 µL | 867.431 µL | 1.735 mL |
10 mM | 8.674 µL | 43.372 µL | 86.743 µL | 433.715 µL | 867.431 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.3 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 73000 |
Excitation (nm) | 499 |
Emission (nm) | 520 |
Quantum yield | 0.921 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor®488-dUTP *1 mM in Tris Buffer (pH 7.5)* | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
References
View all 6 references: Citation Explorer
Pegylated-Catalase Is Protective in Lung Ischemic Injury and Oxidative Stress.
Authors: Kim, Jung-Lye and Reader, Brenda F and Dumond, Curtis and Lee, Yonggyu and Mokadam, Nahush A and Black, Sylvester M and Whitson, Bryan A
Journal: The Annals of thoracic surgery (2021): 1019-1027
Authors: Kim, Jung-Lye and Reader, Brenda F and Dumond, Curtis and Lee, Yonggyu and Mokadam, Nahush A and Black, Sylvester M and Whitson, Bryan A
Journal: The Annals of thoracic surgery (2021): 1019-1027
Visualization of heavy ion tracks by labeling 3'-OH termini of induced DNA strand breaks.
Authors: Konishi, Teruaki and Takeyasu, Akihiro and Natsume, Toshiyuki and Furusawa, Yoshiya and Hieda, Kotaro
Journal: Journal of radiation research (2011): 433-40
Authors: Konishi, Teruaki and Takeyasu, Akihiro and Natsume, Toshiyuki and Furusawa, Yoshiya and Hieda, Kotaro
Journal: Journal of radiation research (2011): 433-40
Peptide amphiphile nanofiber delivery of sonic hedgehog protein to reduce smooth muscle apoptosis in the penis after cavernous nerve resection.
Authors: Bond, Christopher W and Angeloni, Nicholas L and Harrington, Daniel A and Stupp, Samuel I and McKenna, Kevin E and Podlasek, Carol A
Journal: The journal of sexual medicine (2011): 78-89
Authors: Bond, Christopher W and Angeloni, Nicholas L and Harrington, Daniel A and Stupp, Samuel I and McKenna, Kevin E and Podlasek, Carol A
Journal: The journal of sexual medicine (2011): 78-89
A novel screening method for competitive FRET-aptamers applied to E. coli assay development.
Authors: Bruno, John G and Carrillo, Maria P and Phillips, Taylor and Andrews, Carrie J
Journal: Journal of fluorescence (2010): 1211-23
Authors: Bruno, John G and Carrillo, Maria P and Phillips, Taylor and Andrews, Carrie J
Journal: Journal of fluorescence (2010): 1211-23
Competitive FRET-aptamer-based detection of methylphosphonic acid, a common nerve agent metabolite.
Authors: Bruno, John G and Carrillo, Maria P and Phillips, Taylor and Vail, Neal K and Hanson, Douglas
Journal: Journal of fluorescence (2008): 867-76
Authors: Bruno, John G and Carrillo, Maria P and Phillips, Taylor and Vail, Neal K and Hanson, Douglas
Journal: Journal of fluorescence (2008): 867-76
Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection.
Authors: Bruno, John G and Carrillo, Maria P and Phillips, Taylor
Journal: Journal of biomolecular techniques : JBT (2008): 109-15
Authors: Bruno, John G and Carrillo, Maria P and Phillips, Taylor
Journal: Journal of biomolecular techniques : JBT (2008): 109-15
Application notes
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A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
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