XFD™488-dUTP 1 mM in Tris Buffer (pH 7.5)

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Physical properties

Molecular weight	1152.83
Solvent	Water

Spectral properties

Correction Factor (260 nm)	0.3
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	499
Emission (nm)	520
Quantum yield	0.92¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

1152.83

Correction Factor (260 nm)

0.3

Correction Factor (280 nm)

0.11

Extinction coefficient (cm ^-1 M ^-1)

73000

Excitation (nm)

499

Emission (nm)

520

Quantum yield

0.92¹

The dye-modified deoxyuridine 5'-triphosphates are one of the most common methods to produce dye-labelled DNA via the conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This XFD 488-dUTP conjugate has the same chemical structure to ChromaTide™Alexa Fluor 488-5-dUTP. It is used as a green fluorescence color with SpectrumGreen™ filter set. It provides a bright signal that has high photostability and is not affected by pH. ChromaTide™ and Alexa Fluor™ are the trademarks of ThermoFisher, and SpectrumGreen™ is the trademark of Vysis.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of XFD™488-dUTP *1 mM in Tris Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	86.743 µL	433.715 µL	867.431 µL	4.337 mL	8.674 mL
5 mM	17.349 µL	86.743 µL	173.486 µL	867.431 µL	1.735 mL
10 mM	8.674 µL	43.372 µL	86.743 µL	433.715 µL	867.431 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.3
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	499
Emission (nm)	520
Quantum yield	0.92¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor®488-dUTP 1 mM in Tris Buffer (pH 7.5)	491	516	75000¹	0.9¹	0.21	0.11

Images

Figure 1. Chemical structure for XFD™488-dUTP *1 mM in Tris Buffer (pH 7.5)*.

References

View all 6 references: Citation Explorer

Pegylated-Catalase Is Protective in Lung Ischemic Injury and Oxidative Stress.
Authors: Kim, Jung-Lye and Reader, Brenda F and Dumond, Curtis and Lee, Yonggyu and Mokadam, Nahush A and Black, Sylvester M and Whitson, Bryan A
Journal: The Annals of thoracic surgery (2021): 1019-1027

Visualization of heavy ion tracks by labeling 3'-OH termini of induced DNA strand breaks.
Authors: Konishi, Teruaki and Takeyasu, Akihiro and Natsume, Toshiyuki and Furusawa, Yoshiya and Hieda, Kotaro
Journal: Journal of radiation research (2011): 433-40

Peptide amphiphile nanofiber delivery of sonic hedgehog protein to reduce smooth muscle apoptosis in the penis after cavernous nerve resection.
Authors: Bond, Christopher W and Angeloni, Nicholas L and Harrington, Daniel A and Stupp, Samuel I and McKenna, Kevin E and Podlasek, Carol A
Journal: The journal of sexual medicine (2011): 78-89

A novel screening method for competitive FRET-aptamers applied to E. coli assay development.
Authors: Bruno, John G and Carrillo, Maria P and Phillips, Taylor and Andrews, Carrie J
Journal: Journal of fluorescence (2010): 1211-23

Competitive FRET-aptamer-based detection of methylphosphonic acid, a common nerve agent metabolite.
Authors: Bruno, John G and Carrillo, Maria P and Phillips, Taylor and Vail, Neal K and Hanson, Douglas
Journal: Journal of fluorescence (2008): 867-76

Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection.
Authors: Bruno, John G and Carrillo, Maria P and Phillips, Taylor
Journal: Journal of biomolecular techniques : JBT (2008): 109-15