XFD700 acid

• Ex/Em: 696/719 nm
• Extinction coefficient: 205,000 cm^-1M^-1
• Moderate Brightness: Suitable for high-abundance targets, preserving brighter dyes for low-abundance detection.
• Water Soluble: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies
• Panel Optimization: Enhances spectral resolution between APC and APC-iFluor® 780 in multicolor assays.

XFD700, manufactured by AAT Bioquest, is a near-infrared fluorescent dye structurally identical to Alexa Fluor™ 700 (ThermoFisher). It is optimally excited by 633–640 nm laser lines and offers a relatively low fluorescence intensity, making it particularly well-suited for direct imaging of high-abundance targets in both microscopy and flow cytometry. This allows researchers to allocate brighter dyes for detecting lower-abundance antigens, improving overall panel design. XFD700 exhibits excellent aqueous solubility and maintains consistent fluorescence stability across a broad pH range (pH 4–10), ensuring robust and reproducible performance under diverse experimental conditions. Its long-wavelength emission effectively minimizes background autofluorescence, leading to enhanced signal-to-noise ratios, especially in complex biological samples such as tissues. In multicolor flow cytometry panels, XFD700 serves as an ideal option between APC and APC-iFluor® 780, enabling better resolution in complex assays.

XFD700 acid is a non-reactive compound that can be employed as a reference standard in studies utilizing XFD700 conjugates. It is also suitable for use as a control in confocal microscopy, immunocytochemistry (ICC), high-content screening (HCS), flow cytometry, and live cell imaging applications. Furthermore, it can be utilized in the synthesis of activated esters and STP and can be coupled to hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides (e.g., EDAC). This allows for the conjugation of the dye to amino-containing molecules, such as proteins, antibodies, amine-modified oligonucleotides, and peptides.