XFD700 acid
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1102.97 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (280 nm) | 0.07 |
| Extinction coefficient (cm -1 M -1) | 205000 |
| Excitation (nm) | 696 |
| Emission (nm) | 719 |
| Quantum yield | 0.251 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Related products
| Overview |  SDSProtocol |
Molecular weight 1102.97 | Correction Factor (280 nm) 0.07 | Extinction coefficient (cm -1 M -1) 205000 | Excitation (nm) 696 | Emission (nm) 719 | Quantum yield 0.251 |
XFD700 is the same molecule as the Alexa Fluor® 700 (Alexa Fluor® is the trademark of ThermoFisher). It is a bright and photostable near-IR dye that can be excited with a xenon-arc lamp, far-red diode lasers, or dye pumped lasers operating in the 675–700 nm range. It is used for stable signal generation in imaging and flow cytometry, and can be conjugated to antibodies, peptides, proteins, tracers, and amplification substrates. XFD700 has an absorption maximum at 702 nm and an emission maximum at 723 nm. It is compatible with all common excitation sources and instruments and can replace standard fluorophores such as Cy5.5 or IR680 for most applications.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of XFD700 acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 90.664 µL | 453.321 µL | 906.643 µL | 4.533 mL | 9.066 mL |
| 5 mM | 18.133 µL | 90.664 µL | 181.329 µL | 906.643 µL | 1.813 mL |
| 10 mM | 9.066 µL | 45.332 µL | 90.664 µL | 453.321 µL | 906.643 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (280 nm) | 0.07 |
| Extinction coefficient (cm -1 M -1) | 205000 |
| Excitation (nm) | 696 |
| Emission (nm) | 719 |
| Quantum yield | 0.251 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| XFD532 acid *Same Structure to Alexa Fluor™ 532 acid* | 317 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
| XFD488 acid *Same Structure to Alexa Fluor™ 488 acid* | 490 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
| XFD350 acid *Same Structure to Alexa Fluor™ 350 acid* | 303 | 439 | 19000 | - | 0.25 | 0.19 |
| XFD546 acid *Same Structure to Alexa Fluor™ 546 acid* | 323 | 572 | 112000 | 0.791 | 0.21 | 0.12 |
| XFD568 acid *Same Structure to Alexa Fluor™ 568 acid* | 546 | 603 | 91300 | 0.691 | 0.45 | 0.46 |
| XFD514 acid | 315 | 543 | 80000 | - | 0.31 | 0.18 |
| XFD555 acid | 553 | 568 | 155000 | 0.11 | 0.08 | 0.08 |
| XFD647 acid | 650 | 671 | 270000 | 0.331 | - | 0.03 |
| XFD750 acid | 752 | 776 | 290000 | 0.121 | - | 0.04 |
Show More (11) | ||||||
Images
Figure 1. XFD700 is the same molecule as the Alexa Fluor® 700. It reacts with an amino compound in the presence of a catalyst such as EDAC etc.
References
View all 40 references: Citation Explorer
Visualization of preimplantation uterine fluid absorption in mice using Alexa Fluor™ 488 hydrazide.
Authors: Li, Yuehuan and Martin, Taylor Elijah and Hancock, Jonathan Matthew and Li, Rong and Viswanathan, Suvitha and Lydon, John P and Zheng, Yi and Ye, Xiaoqin
Journal: Biology of reproduction (2022)
Authors: Li, Yuehuan and Martin, Taylor Elijah and Hancock, Jonathan Matthew and Li, Rong and Viswanathan, Suvitha and Lydon, John P and Zheng, Yi and Ye, Xiaoqin
Journal: Biology of reproduction (2022)
Alexa Fluor 488-conjugated cholera toxin subunit B optimally labels neurons 3-7 days after injection into the rat gastrocnemius muscle.
Authors: Cui, Jing-Jing and Wang, Jia and Xu, Dong-Sheng and Wu, Shuang and Guo, Ya-Ting and Su, Yu-Xin and Liu, Yi-Han and Wang, Yu-Qing and Jing, Xiang-Hong and Bai, Wan-Zhu
Journal: Neural regeneration research (2022): 2316-2320
Authors: Cui, Jing-Jing and Wang, Jia and Xu, Dong-Sheng and Wu, Shuang and Guo, Ya-Ting and Su, Yu-Xin and Liu, Yi-Han and Wang, Yu-Qing and Jing, Xiang-Hong and Bai, Wan-Zhu
Journal: Neural regeneration research (2022): 2316-2320
Click-iT ® Plus OPP Alexa Fluor ® Protein Synthesis Assay in Embryonic Cells.
Authors: Li, Yan and Ji, Xu and Chang, Lu and Tang, Jianan and Hua, Min-Min and Liu, Jing and O'Neill, Christopher and Huang, Xuefeng and Jin, Xingliang
Journal: Bio-protocol (2022): e4441
Authors: Li, Yan and Ji, Xu and Chang, Lu and Tang, Jianan and Hua, Min-Min and Liu, Jing and O'Neill, Christopher and Huang, Xuefeng and Jin, Xingliang
Journal: Bio-protocol (2022): e4441
Evaluation of Blood-Brain Barrier Integrity Using Vascular Permeability Markers: Evans Blue, Sodium Fluorescein, Albumin-Alexa Fluor Conjugates, and Horseradish Peroxidase.
Authors: Ahishali, Bulent and Kaya, Mehmet
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 87-103
Authors: Ahishali, Bulent and Kaya, Mehmet
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 87-103
Effects of Viscosity and Refractive Index on the Emission and Diffusion Properties of Alexa Fluor 405 Using Fluorescence Correlation and Lifetime Spectroscopies.
Authors: van Zanten, Camila and Melnikau, Dzmitry and Ryder, Alan G
Journal: Journal of fluorescence (2021): 835-845
Authors: van Zanten, Camila and Melnikau, Dzmitry and Ryder, Alan G
Journal: Journal of fluorescence (2021): 835-845
Molecular and Spectroscopic Characterization of Green and Red Cyanine Fluorophores from the Alexa Fluor and AF Series*.
Authors: Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2021)
Authors: Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2021)
Molecular and Spectroscopic Characterization of Green and Red Cyanine Fluorophores from the Alexa Fluor and AF Series.
Authors: Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2021): 1546
Authors: Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2021): 1546
Hot-Band Anti-Stokes Fluorescence Properties of Alexa Fluor 568.
Authors: Gajdos, Tamás and Hopp, Béla and Erdélyi, Miklós
Journal: Journal of fluorescence (2020): 437-443
Authors: Gajdos, Tamás and Hopp, Béla and Erdélyi, Miklós
Journal: Journal of fluorescence (2020): 437-443
Photo-isomerization of the Cyanine Dye Alexa-Fluor 647 (AF-647) in the Context of dSTORM Super-Resolution Microscopy.
Authors: Karlsson, Joshua K G and Laude, Alex and Hall, Michael J and Harriman, Anthony
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2019): 14983-14998
Authors: Karlsson, Joshua K G and Laude, Alex and Hall, Michael J and Harriman, Anthony
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2019): 14983-14998
Observing the Reversible Single Molecule Electrochemistry of Alexa Fluor 647 Dyes by Total Internal Reflection Fluorescence Microscopy.
Authors: Fan, Sanjun and Webb, James E A and Yang, Ying and Nieves, Daniel J and Gonçales, Vinicius R and Tran, Jason and Hilzenrat, Geva and Kahram, Mohaddeseh and Tilley, Richard D and Gaus, Katharina and Gooding, J Justin
Journal: Angewandte Chemie (International ed. in English) (2019): 14495-14498
Authors: Fan, Sanjun and Webb, James E A and Yang, Ying and Nieves, Daniel J and Gonçales, Vinicius R and Tran, Jason and Hilzenrat, Geva and Kahram, Mohaddeseh and Tilley, Richard D and Gaus, Katharina and Gooding, J Justin
Journal: Angewandte Chemie (International ed. in English) (2019): 14495-14498