XFD700 acid

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Physical properties

Molecular weight	1102.97
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.07
Extinction coefficient (cm ^-1 M ^-1)	205000
Excitation (nm)	696
Emission (nm)	719
Quantum yield	0.25¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD700 acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	90.664 µL	453.321 µL	906.643 µL	4.533 mL	9.066 mL
5 mM	18.133 µL	90.664 µL	181.329 µL	906.643 µL	1.813 mL
10 mM	9.066 µL	45.332 µL	90.664 µL	453.321 µL	906.643 µL

Molarity calculator

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Spectrum

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Spectral properties

Correction Factor (280 nm)	0.07
Extinction coefficient (cm ^-1 M ^-1)	205000
Excitation (nm)	696
Emission (nm)	719
Quantum yield	0.25¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
XFD532 acid Same Structure to Alexa Fluor™ 532 acid	534	553	81000	0.61¹	0.24	0.09
XFD488 acid Same Structure to Alexa Fluor™ 488 acid	499	520	71000	0.92¹	0.30	0.11
XFD350 acid Same Structure to Alexa Fluor™ 350 acid	343	441	19000	-	0.25	0.19
XFD546 acid Same Structure to Alexa Fluor™ 546 acid	561	572	112000	0.79¹	0.21	0.12
XFD568 acid Same Structure to Alexa Fluor™ 568 acid	579	603	91300	0.69¹	0.45	0.46
XFD514 acid	518	543	80000	-	0.31	0.18
XFD555 acid	553	568	155000	0.1¹	0.08	0.08
XFD647 acid	650	671	270000	0.33¹	-	0.03
XFD750 acid	752	776	290000	0.12¹	-	0.04
ICG acid	789	813	230000	-	-	0.076
Cy3NS acid CAS 1032678-01-5	555	569	150000¹	0.15¹	0.07	0.073
Cy5NS acid	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
Cy7NS acid	756	779	250000	0.3	0.05	0.036
AMCA acid CAS#: 106562-32-7	346	434	19000	-	-	0.153
EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] CAS 50402-56-7	336	455	5900	-	-	0.107
EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid]	336	455	5900	-	-	0.107
DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] CAS 6268-49-1	-	-	-	-	-	0.516
BCECF acid CAS#: 85138-49-4	504	527	90000	-	-	-
Cy3B acid	560	571	120000¹	0.58¹	0.048	0.069
QXY7 acid [equivalent to QSY-7 acid]	-	-	90000¹	-	-	0.22
OG514 acid [equivalent to Oregon Green® 514 carboxylic acid]	513	533	85000	-	-	0.19
Show More (12)

Images

Figure 1. XFD700 is the same molecule as the Alexa Fluor® 700. It reacts with an amino compound in the presence of a catalyst such as EDAC etc.

References

View all 40 references: Citation Explorer

Visualization of preimplantation uterine fluid absorption in mice using Alexa Fluor™ 488 hydrazide.
Authors: Li, Yuehuan and Martin, Taylor Elijah and Hancock, Jonathan Matthew and Li, Rong and Viswanathan, Suvitha and Lydon, John P and Zheng, Yi and Ye, Xiaoqin
Journal: Biology of reproduction (2022)

Alexa Fluor 488-conjugated cholera toxin subunit B optimally labels neurons 3-7 days after injection into the rat gastrocnemius muscle.
Authors: Cui, Jing-Jing and Wang, Jia and Xu, Dong-Sheng and Wu, Shuang and Guo, Ya-Ting and Su, Yu-Xin and Liu, Yi-Han and Wang, Yu-Qing and Jing, Xiang-Hong and Bai, Wan-Zhu
Journal: Neural regeneration research (2022): 2316-2320

Click-iT ® Plus OPP Alexa Fluor ® Protein Synthesis Assay in Embryonic Cells.
Authors: Li, Yan and Ji, Xu and Chang, Lu and Tang, Jianan and Hua, Min-Min and Liu, Jing and O'Neill, Christopher and Huang, Xuefeng and Jin, Xingliang
Journal: Bio-protocol (2022): e4441

Evaluation of Blood-Brain Barrier Integrity Using Vascular Permeability Markers: Evans Blue, Sodium Fluorescein, Albumin-Alexa Fluor Conjugates, and Horseradish Peroxidase.
Authors: Ahishali, Bulent and Kaya, Mehmet
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 87-103

Effects of Viscosity and Refractive Index on the Emission and Diffusion Properties of Alexa Fluor 405 Using Fluorescence Correlation and Lifetime Spectroscopies.
Authors: van Zanten, Camila and Melnikau, Dzmitry and Ryder, Alan G
Journal: Journal of fluorescence (2021): 835-845

Molecular and Spectroscopic Characterization of Green and Red Cyanine Fluorophores from the Alexa Fluor and AF Series*.
Authors: Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2021)

Molecular and Spectroscopic Characterization of Green and Red Cyanine Fluorophores from the Alexa Fluor and AF Series.
Authors: Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2021): 1546

Hot-Band Anti-Stokes Fluorescence Properties of Alexa Fluor 568.
Authors: Gajdos, Tamás and Hopp, Béla and Erdélyi, Miklós
Journal: Journal of fluorescence (2020): 437-443

Photo-isomerization of the Cyanine Dye Alexa-Fluor 647 (AF-647) in the Context of dSTORM Super-Resolution Microscopy.
Authors: Karlsson, Joshua K G and Laude, Alex and Hall, Michael J and Harriman, Anthony
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2019): 14983-14998

Observing the Reversible Single Molecule Electrochemistry of Alexa Fluor 647 Dyes by Total Internal Reflection Fluorescence Microscopy.
Authors: Fan, Sanjun and Webb, James E A and Yang, Ying and Nieves, Daniel J and Gonçales, Vinicius R and Tran, Jason and Hilzenrat, Geva and Kahram, Mohaddeseh and Tilley, Richard D and Gaus, Katharina and Gooding, J Justin
Journal: Angewandte Chemie (International ed. in English) (2019): 14495-14498