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How to fluorescently label exosomes?
Posted August 30, 2019
Answer
You can use any one of these lipophilic fluorescent stains for labeling membranes and other hydrophobic structures, such as exosomes:
- DiOC16(3) perchlorate – Abs/Em maxima = 482/500 nm
- DiI perchlorate – Abs/Em maxima = 549/563 nm
- DiD perchlorate – Abs/Em maxima = 645/663 nm
- DiR iodide – Abs/Em maxima = 754/778 nm
The distinct fluorescence color of DiO (green fluorescence), DiI (orange fluorescence), DiD (red fluorescence) and DiR (deep red fluorescence) provide convenient tools for multicolor imaging and flow cytometric analysis.
For more lipophilic fluorescent stains, click here.
Additional resources
DiOC16(3) perchlorate [3,3-Dihexadecyloxacarbocyanine perchlorate]
DiR iodide [1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide]
DiD labeling solution [1,1-Dioctadecyl-3,3,3,3-tetramethylindodicarbocyanine] *5 mM DMSO solution*
DiIC12(3)-DS [1,1-Diododecyl-3,3,3,3-tetramethylindocarbocyanine-5,5-disulfonic acid]