AAT Bioquest

How to fluorescently label exosomes?

Posted August 30, 2019

You can use any one of these lipophilic fluorescent stains for labeling membranes and other hydrophobic structures, such as exosomes:
The distinct fluorescence color of DiO (green fluorescence), DiI (orange fluorescence), DiD (red fluorescence) and DiR (deep red fluorescence) provide convenient tools for multicolor imaging and flow cytometric analysis.
For more lipophilic fluorescent stains, click here.
Additional resources

DiOC16(3) perchlorate [3,3-Dihexadecyloxacarbocyanine perchlorate]

DiR iodide [1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide]

DiD labeling solution [1,1-Dioctadecyl-3,3,3,3-tetramethylindodicarbocyanine] *5 mM DMSO solution*

DiIC12(3)-DS [1,1-Diododecyl-3,3,3,3-tetramethylindocarbocyanine-5,5-disulfonic acid]