What is the difference between PCR and molecular cloning regarding DNA replication?
Posted June 22, 2020
- Molecular cloning replicates DNA within in a living cell, while PCR replicates DNA in an in vitro solution, free of living cells.
- Molecular cloning involves cutting and pasting the sequences, while PCR amplifies DNA by copying an existing sequence.
- DNA cloned by molecular cloning is usually faithfully copied and fully functional, whereas PCR introduces errors in sequence, resulting in mutations.