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What is the difference between SDS-PAGE and western blot?
Posted June 1, 2020
Answer
SDS-PAGE is an electrophoresis method that separates proteins by mass. Western blot is an analytical technique to identify the presence of a specific protein within a complex mixture of proteins, where gel electrophoresis is usually used as the first step in procedure to separate the protein of interest. SDS-PAGE is by far the most common type of gel electrophoresis being used in western blot.
Additional resources
Antibody development servicesGelite™ Green Nucleic Acid Gel Staining KitMahmood, T., & Yang, P. C. (2012). Western blot: technique, theory, and trouble shooting. North American journal of medical sciences, 4(9), 429.Sambrook, J., & Russell, D. W. (2006). SDS-polyacrylamide gel electrophoresis of proteins. CSH Protoc, 2006(4), pdb-prot4540.SDS-PAGE SDS Running Buffer (10x) Preparation and Recipe