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What is the difference between bicarbonate and PBS as a coating buffer in ELISA assay?
Posted April 24, 2020

Answer

PBS and bicarbonate buffer, which are two of the most commonly used coating buffers for ELISAs, differ in their pH—while PBS has a pH of 7.4, bicarbonate buffer has a pH of 9.5. For many antigens, the higher pH of a bicarbonate buffer assists in hydrophobic reactions between amino acids side chains and the polystyrene surface of the plate, thus helping in the coating process.