What is the difference between compensation and spectral unmixing?
Posted June 22, 2020
Both compensation and spectral unmixing are processes aiming to remove the interfering signals from other fluorescent molecules, which can usually be achieved automatically by appropriate software. Compensation uses the spillover matrix while spectral unmixing uses reference spectra for calculation, which are in fact mathematically similar. The main difference between these two methods is that spectral unmixing handles data from more detectors than compensation, and separates the individual spectral fingerprints rather than intensities at a specific wavelength.
Fundamentals of Flow Cytometry
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Nolan, J. P., & Condello, D. (2013). Spectral flow cytometry. Current protocols in cytometry, 63(1), 1-27.