AAT Bioquest

What is the difference between trypsin and chymotrypsin?

Posted July 22, 2020


Trypsin and chymotrypsin are two very similar digestive enzymes that hydrolyze proteins into amino acids. Although they share similar structure and function, there are still some differences between these two enzymes.

  • Specificity: Trypsin hydrolyzes peptide bond at the C-terminal side of basic amino acids such as lysine and arginine, whereas chymotrypsin attacks the C-terminal side of aromatic amino acids like phenylalanine, tryptophan, and tyrosine. This is the main difference between these two enzymes.
  • Activation: The inactive form of trypsin, trypsinogen, is activated by enterokinase, while chymotrypsinogen is activated by trypsin.
Additional resources

Amplite™ Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*

Amplite™ Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*

Lodish, H., Berk, A., Kaiser, C. A., Krieger, M., Scott, M. P., Bretscher, A., ... & Matsudaira, P. (2008). Molecular cell biology. Macmillan.

Berg, J. M., Tymoczko, J. L., & Stryer, L. (2008). Biochemistry (Loose-Leaf). Macmillan.