logo
AAT Bioquest

What is the working solution concentration for 6-ROX glycine?

Posted June 1, 2018


How much should we add to our 1 mL PCR mix?

Answer
The working soluition concentration for 6-ROX glycine is ~500nM.
 
When calibrating an ABI real-time PCR insturment, we recommend performing a 40X to 50X dilution of 6-ROX glycine.
Additional resources

6-ROX glycine *25 uM fluorescence reference solution for PCR reactions*

6-ROXtra™ fluorescence reference solution *25 uM for PCR reactions*