AAT Bioquest

Can homogeneous mixtures be separated?

Posted April 14, 2023


Yes, homogeneous mixtures can be separated by distillation, filtration, crystallization, or chromatography. In homogeneous mixtures, the individual components are uniformly distributed throughout the mass and are indistinguishable. The technique used to separate homogeneous mixtures depends on the properties of the individual components of the mixture. 

Distillation: Distillation is used to separate a mixture if the individual components have different boiling points. 

Solvent extraction: This technique of separating mixtures is based on the relative solubility of the individual components in different immiscible liquids. 

Precipitation/crystallization reactions: This is a two-step separating technique that can be used if one of the components forms a solid salt due to changes in temperature or concentration. The solid salt can be separated from the liquid component by simple decantation, filtration or centrifugation. 

Chromatography: Chromatography is based on the mobility of the phases, for example- gas chromatography or liquid chromatography. 

Electrophoresis: Electrophoresis can be used to separate macromolecules in a fluid or gel based on their size, charge, and binding affinity.

Additional resources

Liquid-Liquid Phase Separation in Supermicrometer and Submicrometer Aerosol Particles

Buffers and Lab Consumables

ReadiUse™ hydrogen peroxide solution *50 mM calibrated and stabilized solution*