AAT Bioquest

How should an antifade reagent be chosen for a specific experiment or analysis?

Posted April 13, 2023


An antifade reagent should optimally be chosen to have no affinity for the target epitopes, stabilization of cellular morphology, and high binding rates to non-target reactive sites. This ensures that there is minimal loss of fluorescence due to photobleaching, and thus it is important to pick the correct antifade reagent. For example, SlowFade Glass is a commonly used antifade reagent for samples with imaging depths between 0-500 micrometers (including cultured cells, thin and thick tissue sections, and organoids. This antifade reagent is preferred  over other reagents because it preserves signals across the entire visible spectrum and generates minimal quenching of the initial fluorescent signals. The reagent ensures protection against fading across the visible and IR spectra, and can be used with most fluorescent dyes.  

Additional resources

An introduction to Performing Immunofluorescence Staining

FluoroQuest™ Anti-fading Mounting Medium with DAPI