AAT Bioquest

What are some common anti-fading techniques?

Posted April 13, 2023


There are 3 steps to follow for a common antifading technique. In step 1, one must place the sample on the cover slide and finish all staining before or after this step. In step 2, the antifade mountant is applied over the sample. If it is a hard setting mount, it should be cured overnight in open air. In step 3, (for a hard-setting mountant) one should add a drop of 100% glycerol to the mounted specimen and add a coverslip. If a mountant is soft-setting, the slides should be imaged immediately. They can be washed away and the sample can be restrained or used for PCR or single-cell RNA-sequencing. Hard-setting mountants are used for long-term storage of samples.

Additional resources

Anti-Fading Media for Live Cell GFP Imaging

FluoroQuest™ Anti-fading Mounting Medium with DAPI