AAT Bioquest

What are some common components of an antifade mounting medium?

Posted April 13, 2023


Mounting mediums are made of 2 or 3 main components: a base, an antifade reagent, and a sealer to set. Sodium azide is typically used as a preservative. The base component of a mounting medium is either aqueous (RI 1.33), glycerol (RI 1.47), plastic (RI 1.51), natural oil (1.53). The base component is the major determinant of the RI of the medium. Glycerol may be incompatible with samples containing lipophilic plasma membrane stains like DiL. The majority of antifade reagents are reactive oxygen species scavengers. Some antifade reagents will quench fluorescence of the dye such as NPG and DABCO. DPX (distyrene tricresyl phosphate xylene) is a commonly used plasticizer and works effectively with Lissamine rhodamine and TRITC.   

Additional resources

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