What are the advantages of Crispr-Cas9?

One advantage of CRISPR-Cas9 is the flexible design. CRISPR-Cas9 gene editing requires just two main components: the Cas nuclease and a guide RNA. Researchers have the option to use either the two-part crRNA-tracrRNA system or a single-guide RNA (sgRNA). Once these components join together, they form a ribonucleoprotein complex, allowing the guide RNA to target the desired sequence for editing. Another advantage is CRISPR-Cas9 enables the simultaneous editing of multiple genes by designing sgRNAs to target different genetic locations. This feature makes CRISPR-Cas9 an efficient method for manipulating several regions in the genome at once. Another pro is that CRISPR-Cas9 is a highly cost-effective gene editing solution compared to alternatives like transcription activator-like effector nucleases (TALENs) and zinc finger nucleases (ZFNs). This is primarily because these other methods require protein engineering processes, which can significantly increase the overall cost involved.