AAT Bioquest

What are the common fluorescein applications?

Posted November 10, 2022


Fluorescein is a highly fluorescent substance with an absorption maximum at 494 nm and emission maximum of 512 nm in water. 

Fluorescein and its derivatives are commonly used in the following applications:  

  • Microscopy
  • Analytical chemistry as an indicator in silver nitrate titrations
  • Forensics and serology to detect blood traces
  • A specific type of dye laser as the gain medium
  • Dye tracing
  • Environmental testing simulations 

The sodium salt of fluorescein, fluorescein sodium, is used extensively in ophthalmology and optometry as a diagnostic tool.

Additional resources

An efficient method for FITC labelling of proteins using tandem affinity purification


Fluorescein *CAS 2321-07-5*