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What are the common fluorescein applications?
Posted November 10, 2022
Chemical ReagentsClassic DyesFlow Cytometry ReagentsFluoresceinsPhysiological ProbesSpectral Flow Cytometryimmunofluorescence
Answer
Fluorescein is a highly fluorescent substance with an absorption maximum at 494 nm and emission maximum of 512 nm in water. 
Fluorescein and its derivatives are commonly used in the following applications:  
  • Microscopy
  • Analytical chemistry as an indicator in silver nitrate titrations
  • Forensics and serology to detect blood traces
  • A specific type of dye laser as the gain medium
  • Dye tracing
  • Environmental testing simulations 
The sodium salt of fluorescein, fluorescein sodium, is used extensively in ophthalmology and optometry as a diagnostic tool.
Additional resources
An efficient method for FITC labelling of proteins using tandem affinity purification FluoresceinsFluorescein *CAS 2321-07-5*

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