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AAT Bioquest

What are the types of chemical reagents used in the staining process of the tissues?

Posted March 22, 2023


Answer

During the staining process of tissues, hematoxylin and eosin are commonly used chemical reagents. Hematoxylin is a basic dye that stains the nuclei a blue/purple color. It also stains RNA in ribosomes and rough endoplasmic reticulum. Eosin is a counterstain used after hematoxylin, and stains the cell’s cytoplasmic organelles a pink/red color.

Additional resources

Histological Stains: A Literature Review and Case Study

Immunohistochemistry (IHC)

Stayright™ Purple HRP Staining Kit