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What are the types of probes that can be used in performing in situ hybridization?
Posted March 27, 2024
DNA and RNA QuantitationFISHFluorescenceNucleic AcidsNucleusPhysiological Probesin situ hybridization
Answer

The types of probes which are utilized carrying out in situ hybridization include: 

  1. dsDNA probes
  2. RNA probes
  3. ssDNA probes
  4. synthetic oligonucleotides 
Additional resources
Technical Review: In Situ HybridizationFluorescent in situ hybridization (FISH) ReadiLink™ iFluor® 488 FISH Fluorescence Imaging Kit

Which is the most complex step of FISH?What are the differences between FISH and ISH?What are the advantages of Fluorescence in situ hybridization (FISH)?What are the disadvantages of using dsDNA probes for performing in situ hybridization?What are the disadvantages of using RNA probes for performing in situ hybridization?