AAT Bioquest

What is a critical regulator of mitosis in all eukaryotic cells?

Posted November 30, 2023


The critical regulator of mitosis in all eukaryotic cells are cyclin-dependent kinases (CDKs). These cyclins form complexes with S phase cyclins (S-CDKS) and mitotic cyclins (M-CDKS) to modulate DNA replication. S-CDK functions to prevent re-replication from occurring after the S phase is completed. M-CDK enables the transition of the cell into mitosis, and phosphorylates Cdc6 and Mcm proteins. The primary mitotic kinase in mitosis is CDK-1/cyclin B (which is a type of M-CDK). The CDK-1/cyclin B complex triggers the cell’s entry into mitosis by phosphorylating and activating enzymes which modulate chromatin condensation, spindle formation, and the breakdown of the nuclear envelope.     

Additional resources

Regulators of Cell Cycle Progression

Cell Cycle Assays

Cell Meter™ Fluorimetric Live Cell Cycle Assay Kit *Green Fluorescence Optimized for Flow Cytometry*