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What is needed for Sanger sequencing?
Posted July 11, 2022
Coronavirus (SARS-CoV-2)DNA and RNA QuantitationPhysiological ProbesNucleus
Answer

Sanger sequencing requires: 

  • A DNA template
  • A sequencing primer, which is a short piece of DNA
  • A thermostable DNA polymerase, which is the enzyme that copies DNA
  • Nucleotides (dNTPS)
  • dideoxynucleotides (ddNTPs) 
  • Buffer
Additional resources
A simplified Sanger sequencing method for full genome sequencing of multiple subtypes of human influenza A virusesMagaDye™ 4 Color Sanger Sequencing Terminator Kit

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