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AMP-Fluorescein conjugate calibrator

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Fax1-800-609-2943
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Physical properties
Molecular weight~800
SolventDMSO
Spectral properties
Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


See also: Fluoresceins
Molecular weight
~800
Absorbance (nm)
487
Correction Factor (260 nm)
0.32
Correction Factor (280 nm)
0.35
Extinction coefficient (cm -1 M -1)
800001
Excitation (nm)
498
Emission (nm)
517
Quantum yield
0.79001, 0.952
AMP-Fluorescein conjugate calibrator is desigend to use with our green fluorescent FAM-cAMP PDE IV substrate (#Cat: 13602), a cAMP derivative that is a specific substrate for phosphodiesterase (PDE) IV. This AMP-Fluorescein conjugate calibrator is the hydrolyzed product of FAM-cAMP PDE IV substrate by PDE IV. It is used to calibrate the hydrolysis rate of FAM-cAMP PDE IV substrate by PDE IV.

Spectrum


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spectrum

Spectral properties

Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
GMP-Fluorescein conjugate calibrator4985178000010.79001, 0.9520.320.35

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References


View all 34 references: Citation Explorer
Importance of cAMP-response element-binding protein in regulation of expression of the murine cyclic nucleotide phosphodiesterase 3B (Pde3b) gene in differentiating 3T3-L1 preadipocytes
Authors: Liu H, Tang JR, Choi YH, Napolitano M, Hockman S, Taira M, Degerman E, Manganiello VC.
Journal: J Biol Chem (2006): 21096
Immunohistochemical distribution of cAMP- and cGMP-Phosphodiesterase (PDE) isoenzymes in the human prostate
Authors: Sampaio FJ., undefined
Journal: Int Braz J Urol (2006): 368
Scanning peptide array analyses identify overlapping binding sites for the signalling scaffold proteins, beta-arrestin and RACK1, in cAMP-specific phosphodiesterase PDE4D5
Authors: Bolger GB, Baillie GS, Li X, Lynch MJ, Herzyk P, Mohamed A, Mitchell LH, McCahill A, Hundsrucker C, Klussmann E, Adams DR, Houslay MD.
Journal: Biochem J (2006): 23
cAMP phosphodiesterase-4A1 (PDE4A1) has provided the paradigm for the intracellular targeting of phosphodiesterases, a process that underpins compartmentalized cAMP signalling
Authors: Huston E, Houslay TM, Baillie GS, Houslay MD.
Journal: Biochem Soc Trans (2006): 504
Expression and Activity of cAMP Phosphodiesterase Isoforms in Pulmonary Artery Smooth Muscle Cells from Patients with Pulmonary Hypertension: Role for PDE1
Authors: Murray F, Patel HH, Suda RY, Zhang S, Thistlethwaite P, Yuan JX, Insel PA.
Journal: Am J Physiol Lung Cell Mol Physiol. (2006)
Immunohistochemical distribution of cAMP- and cGMP-phosphodiesterase (PDE) isoenzymes in the human prostate
Authors: Uckert S, Oelke M, Stief CG, Andersson KE, Jonas U, Hedlund P.
Journal: Eur Urol (2006): 740
The Dunce cAMP phosphodiesterase PDE-4 negatively regulates G alpha(s)-dependent and G alpha(s)-independent cAMP pools in the Caenorhabditis elegans synaptic signaling network
Authors: Charlie NK, Thomure AM, Schade MA, Miller KG.
Journal: Genetics (2006): 111
Phosphodiesterase-5 Gln817 is critical for cGMP, vardenafil, or sildenafil affinity: its orientation impacts cGMP but not cAMP affinity
Authors: Zoraghi R, Corbin JD, Francis SH.
Journal: J Biol Chem (2006): 5553
DdPDE4, a novel cAMP-specific phosphodiesterase at the surface of dictyostelium cells
Authors: Bader S, Kortholt A, Snippe H, Van Haastert PJ.
Journal: J Biol Chem (2006): 20018
Expression of cAMP and cGMP-phosphodiesterase isoenzymes 3, 4, and 5 in the human clitoris: immunohistochemical and molecular biology study
Authors: Oelke M, Hedlund P, Albrecht K, Ellinghaus P, Stief CG, Jonas U, Andersson KE, Uckert S.
Journal: Urology (2006): 1111