HEX-dT Phosphoramidite
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1632.23 |
Solvent | MeCN |
Spectral properties
Correction Factor (280 nm) | 0.15 |
Extinction coefficient (cm -1 M -1) | 73000 |
Excitation (nm) | 533 |
Emission (nm) | 559 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
See also: Peptide and Oligonucleotide Labeling
Molecular weight 1632.23 | Correction Factor (280 nm) 0.15 | Extinction coefficient (cm -1 M -1) 73000 | Excitation (nm) 533 | Emission (nm) 559 |
HEX-dT phosphoramidite contains a DMT group to allow quantification of coupling. It can be inserted into a target sequence as a replacement for a dT residue. It provides a valuable tool to add the HEX tag to a desired location of an oligo sequence. It is complimentary to the commonly used 6-HEX phosphoramidite that contains no DMT group and can only be added at the 5'-terminus, thus terminating synthesis. AAT Bioquest also offer fluorescein-dT phosphoramidite, TET-dT phosphoramidite, biotin-dT phosphoramidite, biotin-dT CPG and Fluorescein-dT CPG.
Calculators
Common stock solution preparation
Table 1. Volume of MeCN needed to reconstitute specific mass of HEX-dT Phosphoramidite to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 61.266 µL | 306.329 µL | 612.659 µL | 3.063 mL | 6.127 mL |
5 mM | 12.253 µL | 61.266 µL | 122.532 µL | 612.659 µL | 1.225 mL |
10 mM | 6.127 µL | 30.633 µL | 61.266 µL | 306.329 µL | 612.659 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (280 nm) | 0.15 |
Extinction coefficient (cm -1 M -1) | 73000 |
Excitation (nm) | 533 |
Emission (nm) | 559 |
References
View all 5 references: Citation Explorer
Solid- and solution-phase synthesis and application of R6G dual-labeled oligonucleotide probes.
Authors: Skoblov, Aleksander Yu and Vichuzhanin, Maxim V and Farzan, Valentina M and Veselova, Olga A and Konovalova, Tatiana A and Podkolzin, Alexander T and Shipulin, German A and Zatsepin, Timofei S
Journal: Bioorganic & medicinal chemistry (2015): 6749-56
Authors: Skoblov, Aleksander Yu and Vichuzhanin, Maxim V and Farzan, Valentina M and Veselova, Olga A and Konovalova, Tatiana A and Podkolzin, Alexander T and Shipulin, German A and Zatsepin, Timofei S
Journal: Bioorganic & medicinal chemistry (2015): 6749-56
Acetylated and methylated β-cyclodextrins as viable soluble supports for the synthesis of short 2′-oligodeoxyribo-nucleotides in solution.
Authors: Molina, Alejandro Gimenez and Kungurtsev, Vyacheslav and Virta, Pasi and Lönnberg, Harri
Journal: Molecules (Basel, Switzerland) (2012): 12102-20
Authors: Molina, Alejandro Gimenez and Kungurtsev, Vyacheslav and Virta, Pasi and Lönnberg, Harri
Journal: Molecules (Basel, Switzerland) (2012): 12102-20
Sorting the unique chirality, right handed single wall carbon nanotubes via the dye modified ssDNA.
Authors: Liu, Ru and Chen, Zhong and Zhao, Feng and Chang, Xueling and Zu, Yan and Gao, Xueyun
Journal: Journal of nanoscience and nanotechnology (2011): 7587-92
Authors: Liu, Ru and Chen, Zhong and Zhao, Feng and Chang, Xueling and Zu, Yan and Gao, Xueyun
Journal: Journal of nanoscience and nanotechnology (2011): 7587-92
A new synthetic protocol for labeled oligonucleotides, using a chemically cleavable universal linker.
Authors: Mahajan, Shweta and Patnaik, S and Kumar, P and Gandhi, R P and Gupta, K C
Journal: Bioorganic & medicinal chemistry (2006): 4302-9
Authors: Mahajan, Shweta and Patnaik, S and Kumar, P and Gandhi, R P and Gupta, K C
Journal: Bioorganic & medicinal chemistry (2006): 4302-9
Amplified fragment length polymorphism (AFLP) protocol for genotyping the malarial parasite Plasmodium falciparum.
Authors: Rubio, J M and Berzosa, P J and Benito, A
Journal: Parasitology (2001): 331-6
Authors: Rubio, J M and Berzosa, P J and Benito, A
Journal: Parasitology (2001): 331-6
Application notes
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A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets