ReadiLink™ Psoralen-A17-Biotin

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	739.89
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C)
UNSPSC	12171501

Overview SDS Protocol

Example protocol

PREPARATION OF STOCK SOLUTIONS

Prepare a ReadiLink™ Psolaren-A17-Biotin stock solution by adding 70 μL (for Cat# 39052) or 350 μL (for Cat# 39053) of DMSO to the vial of ReadiLink™ Psolaren-A17-Biotin.

Note: Any unused stock solution should be divided into single-use aliquots and stored at ≤-20 °C after preparation. Protect from light and avoid repeated freeze-thaw cycles.

SAMPLE EXPERIMENTAL PROTOCOL

The following protocol is to be used as a general guideline and may require optimization for your specific application and experimental system.

Adjust DNA or RNA to the desired concentration (e.g., 20-100 μg/mL) in TE buffer (10 mM Tris, 1 mM EDTA, pH 7.4).

Note: To improve the binding of dye to nucleic acid, DNA can be denatured by boiling for 5 minutes. After boiling, the DNA tube should be quickly cooled by placing it in a dry ice/ethanol bath.
Add 1 μL of ReadiLink™ Psolaren-A17-Biotin stock solution to the DNA or RNA (100 μL volume) and mix well.

Note: A serial dilution of the ReadiLink™ Psolaren-A17-Biotin stock solution may be required when using small reaction volumes.
Irradiate the tube from above using a long wavelength UV light source for at least 20-30 minutes.
To remove any non-reacted ReadiLink™ Psolaren-A17-Biotin, precipitate the sample with 0.5 M NaCl and two volumes of 100% ethanol at -20 °C for a minimum of 30 minutes. After centrifugation, wash the pellet with 70% ethanol and allow it to dry. Dissolve the biotinylated sample in water or a buffer of your choice.
Biotinylated DNA can then be verified using HABA and gel electrophoresis.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiLink™ Psoralen-A17-Biotin to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	135.155 µL	675.776 µL	1.352 mL	6.758 mL	13.516 mL
5 mM	27.031 µL	135.155 µL	270.31 µL	1.352 mL	2.703 mL
10 mM	13.516 µL	67.578 µL	135.155 µL	675.776 µL	1.352 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Images

Figure 1. Chemical structure for ReadiLink™ Psoralen-A17-Biotin.

References

View all 39 references: Citation Explorer

Hidden threat lurking in extensive hand hygiene during the Covid-19 pandemic: investigation of sensitizing molecules in gel products by hyphenated chromatography techniques.
Authors: Salerno, Tania M G and Trovato, Emanuela and Cafeo, Giovanna and Vento, Federica and Zoccali, Mariosimone and Donato, Paola and Dugo, Paola and Mondello, Luigi
Journal: Analytical and bioanalytical chemistry (2023): 3327-3340

Determination of Oxygen Heterocyclic Compounds in Foods Using Supercritical Fluid Chromatography-Tandem Mass Spectrometry.
Authors: Cafeo, Giovanna and Satira, Antonella and Russo, Marina and Mondello, Monica and Dugo, Paola
Journal: Foods (Basel, Switzerland) (2023)

Comparison of the Efficacy and Safety of Bexarotene and Photo(Chemo)Therapy Combination Therapy and Bexarotene Monotherapy for Cutaneous T-Cell Lymphoma.
Authors: Morita, Akimichi and Tateishi, Chiharu and Ikumi, Kyoko and Hayashi, Daisuke and Nakada, Aya and Nishihara, Haruna and Torii, Kan and Nishida, Emi and Tsuruta, Daisuke
Journal: Dermatology and therapy (2022): 615-629

Force-Dependent Intercalative Bulky DNA Adduct Formation Detected by Single-Molecule Stretching.
Authors: Liu, Yajun and Pei, Yufeng and Xu, Jingjing and Cheng, Yuanlei and Tong, Qingyi and You, Huijuan
Journal: Analytical chemistry (2022): 13623-13630

[Alopecia areata with good response to tofacitinib].
Authors: Maksimow, A and Stintzing, D and Wohlrab, J
Journal: Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete (2021): 525-527

Phase II trial of SM-88, a cancer metabolism based therapy, in non-metastatic biochemical recurrent prostate cancer.
Authors: Gartrell, Benjamin A and Roach, Mack and Retter, Avi and Sokol, Gerald H and Del Priore, Giuseppe and Scher, Howard I
Journal: Investigational new drugs (2021): 499-508

Fractional Carbon Dioxide Laser versus Fractional Carbon Dioxide Laser with Autologous Intralesional Platelet-rich Plasma in the Treatment of Stable, Non-segmental Vitiligo: A Randomized Comparative Study.
Authors: Raizada, Annie and Panda, Maitreyee and Singh, Bhabani Stp and Kar, Bikash R
Journal: Journal of cutaneous and aesthetic surgery (2021): 55-63

Comparative Effectiveness and Safety of Methotrexate Versus PUVA in Severe Chronic Stable Plaque Psoriasis.
Authors: Banerjee, Saikat and Das, Suchibrata and Roy, Alok K and Ghoshal, Loknath
Journal: Indian journal of dermatology (2021): 371-377

Design of a Fluorescence Turn-on and Label-free Aptasensor Using the Intrinsic Quenching Power of G-Quadruplex to AMT.
Authors: Wang, Dandan and Geng, Fenghua and Wang, Yongxiang and Ma, Yu and Li, Guixin and Qu, Peng and Shao, Congying and Xu, Maotian
Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2020): 965-970

The European TREatment of ATopic eczema (TREAT) Registry Taskforce survey: prescribing practices in Europe for phototherapy and systemic therapy in adult patients with moderate-to-severe atopic eczema.
Authors: Vermeulen, F M and Gerbens, L A A and Schmitt, J and Deleuran, M and Irvine, A D and Logan, K and Ouwerkerk, W and Vestergaard, C and Flohr, C and Spuls, P I and ,
Journal: The British journal of dermatology (2020): 1073-1082