Name: iFluor® 488 succinimidyl ester
Brand: AAT Bioquest
SKU: 1023
Availability: InStock

iFluor® 488 succinimidyl ester

Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor® 488 dyes are far superior to conjugates of fluorescein derivatives such as FITC. iFluor® 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor® 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor® 488 SE dye is reasonably stable and shows good reactivity and selectivity with protein amino groups. This iFluor® 488 has spectral properties and reactivity similar to Alexa Fluor® 488 NHS ester ( Alexa Fluor® is the trademark of Invitrogen).

HeLa cells were stained with rabbit anti-tubulin followed by iFluor 488 goat anti-rabbit IgG (H+L), and nuclei were stained with Nuclear Red DCS1.

Protocol

COO

COA

Catalog	Size	Price
1023	1 mg	Price
71023	100 ug	Price
71503	5 mg	Price
71553	10 mg	Price

Citations

View all 77 citations: Citation Explorer

Enhanced combinatorial analysis of tumor cell-ECM interactions using design-of-experiment optimized microarrays

Authors:

Kimmel, Hannah and Paxhia, Allison L and Adamji, Zahra and Underhill, Gregory

Journal:

Biofabrication (2025)

Piperine Enhances Mitochondrial Biogenesis to Mitigate Stress in SH-SY5Y Neuroblastoma Cells

Authors:

Saikachain, Nongluk and Charoensawat, Pawitchaya and Tuntoolavest, Oramon and Vejsureeyakul, Napak and Kunpittaya, Teeranart and Poolsri, Wanangkan and Sungkaworn, Titiwat and Saeeng, Rungnapha and Muanprasat, Chatchai and Asavapanumas, Nithi

Journal:

Food Science \& Nutrition (2025): e70637

Multiphasic protein condensation governed by shape and valency

Authors:

Pandey, Vikas and Hosokawa, Tomohisa and Hayashi, Yasunori and Urakubo, Hidetoshi

Journal:

Cell Reports (2025)

Gut-Brain Axis-Based Polygala Tenuifolia and Magnolia Officinalis Improve D-gal-Induced Cognitive Impairment in Mice Through cAMP and NF-$\kappa$B Signaling Pathways

Authors:

Yang, Dan and Li, Wen and Chen, Qiuping and Liu, Si and Peng, Chengjie and Deng, Fengcheng and Meng, Yingqi and Yang, Yang and Yan, Ping and Ao, Hui and others,

Journal:

Drug Design, Development and Therapy (2025): 1869--1894

Axon Trafficking Counteracts Aberrant Protein Aggregation in Neurons

Authors:

Feng, Yu and Luan, Tongshu and Zhang, Zhenda and Wang, Wei and Chu, Yuanyuan and Wan, Sijia and Pan, Xiaorong and Li, Jie and Liu, Yifan and Wang, Tong

Journal:

bioRxiv (2025): 2025--01

References

View all 49 references: Citation Explorer

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Physical properties

Molecular weight	945.07
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9 ¹

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on September 23, 2025