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6-NED, SE [6-NED NHS ester]

Chemical structure for 6-NED, SE [6-NED NHS ester].
Chemical structure for 6-NED, SE [6-NED NHS ester].
Ordering information
Price ()
Catalog Number214
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight644.47
SolventDMSO
Spectral properties
Absorbance (nm)544
Extinction coefficient (cm -1 M -1)740001
Excitation (nm)545
Emission (nm)567
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
644.47
Absorbance (nm)
544
Extinction coefficient (cm -1 M -1)
740001
Excitation (nm)
545
Emission (nm)
567
5'-Labeled primer pairs include a fluorescently labeled oligo with a choice of dye on the 5' end, along with an unlabeled oligo, in each pair. These products can be used in a variety of molecular biological applications. 6-NED is one of the colors used for making the well-known multicolor fluorescent oligo set of 6-FAM, 6-TET, 6-VIC, 6-HEX, 6-NED and 6-PET for use in fragment analysis, gene expression studies, genotyping, and PCR applications. 6 -NED SE is reasonably stable fluorescent probe that is widely used in labeling amino-modified oligos.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-NED, SE [6-NED NHS ester] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM155.166 µL775.831 µL1.552 mL7.758 mL15.517 mL
5 mM31.033 µL155.166 µL310.333 µL1.552 mL3.103 mL
10 mM15.517 µL77.583 µL155.166 µL775.831 µL1.552 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Absorbance (nm)544
Extinction coefficient (cm -1 M -1)740001
Excitation (nm)545
Emission (nm)567

References


View all 13 references: Citation Explorer
Assessment of genetic diversity and bottleneck in Purnathadi buffaloes using short tandem repeat markers.
Authors: Ali, S Sajid and Kuralkar, S V and Das, Ramendra and Raina, Varinder and Kataria, R S and Vohra, Vikas
Journal: Animal biotechnology (2020): 1-12
HLA Class III: A susceptibility region to systemic lupus erythematosus in Tunisian population.
Authors: Hachicha, Hend and Mahfoudh, Nadia and Fourati, Hajer and Elloumi, Nesrine and Marzouk, Sameh and Feki, Sawsan and Fakhfakh, Raouia and Frikha, Faten and Ayadi, Abir and Maatoug, Amira and Gaddour, Lilia and Hakim, Feiza and Bahloul, Zouheir and Makni, Hafedh and Masmoudi, Hatem and Kammoun, Arwa
Journal: PloS one (2018): e0198549
Multicolor-based discrimination of 21 short tandem repeats and amelogenin using four fluorescent universal primers.
Authors: Asari, Masaru and Okuda, Katsuhiro and Hoshina, Chisato and Omura, Tomohiro and Tasaki, Yoshikazu and Shiono, Hiroshi and Matsubara, Kazuo and Shimizu, Keiko
Journal: Analytical biochemistry (2016): 16-22
Multiplex-Ready Technology for mid-throughput genotyping of molecular markers.
Authors: Bonneau, Julien and Hayden, Matthew
Journal: Methods in molecular biology (Clifton, N.J.) (2014): 47-57
Efficient multiplex simple sequence repeat genotyping of the oomycete plant pathogen Phytophthora infestans.
Authors: Li, Ying and Cooke, David E L and Jacobsen, Evert and van der Lee, Theo
Journal: Journal of microbiological methods (2013): 316-22
[Genetic polymorphisms of 15 X-STR loci in Shandong Han population].
Authors: Ma, Teng and Xu, Jing and Yang, Ya-Jun and Sun, Kuan and Xue, Fu-Zhong and Jin, Li and Li, Shi-Lin
Journal: Fa yi xue za zhi (2013): 202-5, 208
A multiplex RTi-PCR reaction for simultaneous detection of Escherichia coli O157:H7, Salmonella spp. and Staphylococcus aureus on fresh, minimally processed vegetables.
Authors: Elizaquível, Patricia and Aznar, Rosa
Journal: Food microbiology (2008): 705-13
Internally controlled triplex quantitative PCR assay for human polyomaviruses JC and BK.
Authors: Dumonceaux, Timothy J and Mesa, Christine and Severini, Alberto
Journal: Journal of clinical microbiology (2008): 2829-36
Clinical validation of a new triplex real-time polymerase chain reaction assay for the detection and discrimination of Herpes simplex virus types 1 and 2.
Authors: Reil, Heide and Bartlime, Ariane and Drerup, Jana and Grewing, Thomas and Korn, Klaus
Journal: The Journal of molecular diagnostics : JMD (2008): 361-7
Developmental validation of a multiplex qPCR assay for assessing the quantity and quality of nuclear DNA in forensic samples.
Authors: Swango, Katie L and Hudlow, William R and Timken, Mark D and Buoncristiani, Martin R
Journal: Forensic science international (2007): 35-45