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Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit
Green Fluorescence
Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. However, during oxidative stress-related states, ROS levels can increase dramatically. The accumulation of ROS results in significant damage to cell structures. The role of oxidative stress in cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory diseases, a number of neurodegenerative diseases and cancer has been well established. The ROS measurement will help to determine how oxidative stress modulates varied intracellular pathways. Cell Meter™ Fluorimetric ROS Assay Kit uses our unique ROS sensor to quantify ROS in live cells. ROS Green is cell-permeable. It generates the green fluorescence when it reacts with ROS. The kit is an optimized "mix and read" assay format that is compatible with HTS liquid handling instruments. The Cell Meter™ Fluorimetric ROS Assay Kit provides a sensitive, one-step fluorimetric assay to detect intracellular ROS in live cells with one hour incubation. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read using either a fluorescence microplate reader or a fluorescence microscope.
Fluorescence images of simultaneous detection of intracellular nitric oxide (NO) and total ROS in RAW 264.7 macrophage. Cells were co-stained with Nitrixyte™ Orange (Red) and Amplite® ROS Green (Green). The cells were then treated with or without 20 µg/mL of lipopolysaccharide (LPS), 1 mM L-arginine (L-Arg) and 50 µM Pyocyanin (Pyo) at 37°C for 16 hours. The fluorescence signals were measured using fluorescence microscope equipped with TRITC (Nitrixyte™ Orange, Red) and FITC (Amplite® ROS Green, Green) filter sets, simultaneously.
Fluorescence images of simultaneous detection of intracellular nitric oxide (NO) and total ROS in RAW 264.7 macrophage. Cells were co-stained with Nitrixyte™ Orange (Red) and Amplite® ROS Green (Green). The cells were then treated with or without 20 µg/mL of lipopolysaccharide (LPS), 1 mM L-arginine (L-Arg) and 50 µM Pyocyanin (Pyo) at 37°C for 16 hours. The fluorescence signals were measured using fluorescence microscope equipped with TRITC (Nitrixyte™ Orange, Red) and FITC (Amplite® ROS Green, Green) filter sets, simultaneously.
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
22900200 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microscope
ExcitationFITC filter
EmissionFITC filter
Recommended plateBlack wall/clear bottom
Fluorescence microplate reader
Excitation490 nm
Emission525 nm
Cutoff515 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
Documents
Protocol
Safety Data Sheet
Certificate of Origin
Certificate of Analysis
Brochure: Reactive Oxygen Species (ROS) Detection
Flyer: Reactive Oxygen Species (ROS)
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Page updated on October 30, 2025