Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Red Fluorescence*
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
UNSPSC | 12352200 |
Overview | ![]() ![]() |
Platform
Fluorescence microscope
Excitation | 520 nm |
Emission | 605 nm |
Recommended plate | Black wall/clear bottom |
Instrument specification(s) | Texas Red filter set |
Fluorescence microplate reader
Excitation | 520 nm |
Emission | 605 nm |
Cutoff | 590 nm |
Recommended plate | Black wall/clear bottom |
Instrument specification(s) | Bottom read mode |
Components
Example protocol
AT A GLANCE
Protocol summary
- Prepare cells in growth medium
- Add Amplite™ ROS Red working solution (100 µL/well for a 96-well plate or 25 µL/well for a 384-well plate)
- Incubate the cells at 37°C for 1 hour
- Treat the cells with test compounds to induce ROS
- Monitor the fluorescence increase (bottom read mode) at Ex/Em= 520/605 nm (Cutoff = 590 nm) or fluorescence microscope with Ex/Em = 520/605 nm filter set
Important notes
Thaw all the kit components at room temperature before starting the experiment.
PREPARATION OF STOCK SOLUTION
1. Amplite™ ROS Red stock solution (500X):
Add 40 µL of DMSO (Component C) into the vial of Amplite™ ROS Red (Component A) and mix well to make 500X Amplite™ ROS Red stock solution. Protect from light. Note: 20 µL of 500X Amplite™ ROS Red stock solution is enough for 1 plate. Note: Unused portion can be aliquoted and stored at < -20 °C for more than one month if the tubes are sealed tightly and kept from light. Avoid repeated freeze-thaw cycles.
PREPARATION OF WORKING SOLUTION
Add 20 µL of 500X Amplite™ ROS Red stock solution into 10 mL of Assay Buffer (Component B) and mix well to make Amplite™ ROS Red working solution. Note: This Amplite™ ROS Red working solution is stable for at least 2 hours at room temperature.
For guidelines on cell sample preparation, please visit
https://www.aatbio.com/resources/guides/cell-sample-preparation.html
SAMPLE EXPERIMENTAL PROTOCOL
- Add 100 µL/well (96-well plate) or 25 µL/well (384-well plate) of Amplite™ ROS Red working solution into the cell plate.
- Incubate the cells in a 5% CO2, 37°C incubator for one hour.
- Treat cells with 20 µL of 11X test compounds (96-well plate) or 10 µL of 6X test compounds (384-well plate) in your desired buffer (such as PBS or HHBS). For control wells (untreated cells), add the corresponding amount of compound buffer.
- To induce ROS, incubate the cell plate at room temperature or in a 5% CO2, 37°C incubator for at least 15 minutes or a desired period of time (30 minutes for Hela cells treated with 1 mM H2O2).
- Monitor the fluorescence increase with a fluorescence microplate reader (bottom read mode) at Ex/Em = 520/605 nm (Cutoff = 590 nm) or observe cells using a fluorescence microscope with Ex/Em = 520/605 nm filter set (Texas Red filter).
Images

Citations
Authors: Zha, Yiwen and Yang, Yongqi and Zhou, Yue and Ye, Bingqian and Li, Hongliang and Liang, Jingyan
Journal: International Journal of Molecular Sciences (2023): 6653
Authors: Yamanobe, Hironaka and Yamamoto, Kenta and Kishimoto, Saki and Nakai, Kei and Oseko, Fumishige and Yamamoto, Toshiro and Mazda, Osam and Kanamura, Narisato
Journal: Molecules (2023): 2935
Authors: Li, Xiaoming and Zhang, Xiang and Hou, Xiaozhi and Bing, Xin and Zhu, Fangyuan and Wu, Xinhao and Guo, Na and Zhao, Hui and Xu, Fenglei and Xia, Ming
Journal: Apoptosis (2022): 1--15
Authors: Tucker, Katherine and Yin, Yajie and Staley, Stuart-Allison and Zhao, Ziyi and Fang, Ziwei and Fan, Yali and Zhang, Xin and Suo, Hongyan and Sun, Wenchuan and Prabhu, Varun Vijay and others,
Journal: American Journal of Cancer Research (2022): 521
Authors: Teng, Yu-Ning and Hung, Chin-Chuan and Kao, Pei-Heng and Chang, Ying-Tzu and Lan, Yu-Hsuan
Journal: Biomedicine \& Pharmacotherapy (2022): 113832
Authors: Cai, Dong-Mei and Mei, Fan-Biao and Zhang, Chao-Jun and An, San-Chun and Lv, Rui-Bo and Ren, Guan-Hua and Xiao, Chan-Chan and Long, Long and Huang, Tian-Ren and Deng, Wei
Journal: Journal of Hepatocellular Carcinoma (2022): 1229--1246
Authors: Inokuchi, Shoichi and Yoshizumi, Tomoharu and Toshima, Takeo and Itoh, Shinji and Yugawa, Kyohei and Harada, Noboru and Mori, Hiroyuki and Fukuhara, Takasuke and Matsuura, Yoshiharu and Mori, Masaki
Journal: Cancer medicine (2021): 1501--1514
Authors: Akintade, Damilare D and Chaudhuri, Bhabatosh
Journal: Biomedicines (2021): 95
Authors: Akintade, Damilare D and Chaudhuri, Bhabatosh
Journal: Cell Biology and Toxicology (2021): 1--10
Authors: Akintade, DD and Chaudhuri, B
Journal: (2021)
References
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