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Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit
Red Fluorescence
Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. However, ROS levels can increase dramatically during oxidative stress-related states, resulting in significant damage to cell structures. The role of oxidative stress in cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory diseases, neurodegenerative disease, and cancer has been well established. Through ROS measurements, researchers can determine how oxidative stress modulates varied intracellular pathways. The Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit uses our unique ROS sensor, Amplite® ROS Red, to quantify ROS in live cells. Amplite® ROS Red is cell-permeable and generates red fluorescence when reacting with ROS. The Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay is easy to perform using an optimized mix-and-read format. It provides a sensitive, one-step fluorimetric assay to detect intracellular ROS in live cells with 1-2 hours incubation. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read using either a fluorescence microplate reader or a fluorescent microscope. It can be used to either quantify ROS activities or screen for ROS inhibitors.
Detection of ROS in Jurkat cells with Cell Meter&trade; Fluorimetric Intracellular Total ROS Activity Assay Kit. Jurkat cells were seeded on the same day at 300,000 cells/100&micro;L/well in a Costar black wall/clear bottom 96-well plate. The ROS assay loading solution (100 &micro;L/well) was added and incubated in a 5% CO2, 37 &deg;C incubator for 1 hour. The cells were treated with or without 1 mM H<sub>2</sub>O<sub>2</sub> for 2 hours. The fluorescence signal was monitored at Ex/Em = 520/605 nm (Cutoff = 590 nm) with bottom read mode using FlexStation (Molecular Devices).
Detection of ROS in Jurkat cells with Cell Meter&trade; Fluorimetric Intracellular Total ROS Activity Assay Kit. Jurkat cells were seeded on the same day at 300,000 cells/100&micro;L/well in a Costar black wall/clear bottom 96-well plate. The ROS assay loading solution (100 &micro;L/well) was added and incubated in a 5% CO2, 37 &deg;C incubator for 1 hour. The cells were treated with or without 1 mM H<sub>2</sub>O<sub>2</sub> for 2 hours. The fluorescence signal was monitored at Ex/Em = 520/605 nm (Cutoff = 590 nm) with bottom read mode using FlexStation (Molecular Devices).
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
22901200 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microscope
Excitation520 nm
Emission605 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Texas Red filter set
Fluorescence microplate reader
Excitation520 nm
Emission605 nm
Cutoff590 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Reactive Oxygen Species (ROS) Detection
Flyer: Reactive Oxygen Species (ROS)
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Page updated on October 29, 2025