Name: Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Orange Fluorescence*
Brand: AAT Bioquest
SKU: 22902
Price: 232 USD
Availability: InStock

Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit

Orange Fluorescence

Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. The accumulation of ROS results in significant damage to cell structures. The role of oxidative stress in cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory diseases, a number of neurodegenerative diseases and cancer has been well established. The ROS measurement will help to determine how oxidative stress modulates varied intracellular pathways. Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit uses our proprietary ROS Brite™ 570 sensor to quantify ROS in live cells. The cell-permeable and non-fluorescent ROS Brite™ 570 exhibits a strong fluorescence signal upon reaction with ROS. ROS Brite™ 570 sensor is localized in the cytoplasm. The fluorescence signal of ROS Brite™ 570 sensor can be measured by fluorescence microscopy, high-content imaging, microplate fluorometry, or flow cytometry. The Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit provides a sensitive, one-step fluorimetric assay to detect intracellular ROS (especially superoxide and hydroxyl radical) in live cells within 1 hour incubation. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format using either a fluorescence microplate reader or a fluorescent microscope with TRITC filter.

Images of HeLa cells stained with Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit in a Costar black wall/clear bottom 96-well plate. A: Untreated control cells. B: Cells treated with 100 µM tert-butyl hydroperoxide (TBHP) for 30 minutes before staining.

Protocol

SDS

Catalog	Size	Price	Quantity
22902	200 Tests	Price

Citations

View all 19 citations: Citation Explorer

Tanreqing injection inhibits stemness and enhances sensitivity of non-small cell lung cancer models to gefitinib through ROS/STAT3 signaling pathway

Authors:

Xiao, Zhenzhen and Ding, Lina and Yu, Yaya and Ma, Changju and Lei, Chenjing and Liu, Yihong and Chang, Xuesong

Journal:

Journal of Cancer (2024): 4259--4274

Palmitic Acid Exerts Anti-Tumorigenic Activities by Modulating Cellular Stress and Lipid Droplet Formation in Endometrial Cancer

Authors:

Zhao, Ziyi and Wang, Jiandong and Kong, Weimin and Newton, Meredith A and Burkett, Wesley C and Sun, Wenchuan and Buckingham, Lindsey and O’Donnell, Jillian and Suo, Hongyan and Deng, Boer and others,

Journal:

Biomolecules (2024): 601

Linoleic acid exhibits anti-proliferative and anti-invasive activities in endometrial cancer cells and a transgenic model of endometrial cancer

Authors:

Qiu, Jianqing and Zhao, Ziyi and Suo, Hongyan and Paraghamian, Sarah E and Hawkins, Gabrielle M and Sun, Wenchuan and Zhang, Xin and Hao, Tianran and Deng, Beor and Shen, Xiaochang and others,

Journal:

Cancer Biology \& Therapy (2024): 2325130

Reduced expression of phosphorylated ataxia-telangiectasia mutated gene is related to poor prognosis and gemcitabine chemoresistance in pancreatic cancer

Authors:

Xun, Jingyu and Ohtsuka, Hideo and Hirose, Katsuya and Douchi, Daisuke and Nakayama, Shun and Ishida, Masaharu and Miura, Takayuki and Ariake, Kyohei and Mizuma, Masamichi and Nakagawa, Kei and others,

Journal:

BMC Cancer (2023): 1--13

Anti-Inflammatory Effects of $\beta$-Cryptoxanthin on 5-Fluorouracil-Induced Cytokine Expression in Human Oral Mucosal Keratinocytes

Authors:

Yamanobe, Hironaka and Yamamoto, Kenta and Kishimoto, Saki and Nakai, Kei and Oseko, Fumishige and Yamamoto, Toshiro and Mazda, Osam and Kanamura, Narisato

Journal:

Molecules (2023): 2935

References

View all 48 references: Citation Explorer

Automatic flow injection based methodologies for determination of scavenging capacity against biologically relevant reactive species of oxygen and nitrogen

Authors:

Magalhaes LM, Lucio M, Segundo MA, Reis S, Lima JL.

Journal:

Talanta (2009): 1219

Reactive oxygen species and yeast apoptosis

Authors:

Perrone GG, Tan SX, Dawes IW.

Journal:

Biochim Biophys Acta (2008): 1354

Sensitive determination of reactive oxygen species by chemiluminescence methods and their application to biological samples and health foods

Authors:

Wada M., undefined

Journal:

Yakugaku Zasshi (2008): 1031

The role of mitochondria in reactive oxygen species metabolism and signaling

Authors:

Starkov AA., undefined

Journal:

Ann N Y Acad Sci (2008): 37

Virion disruption by ozone-mediated reactive oxygen species

Authors:

Murray BK, Ohmine S, Tomer DP, Jensen KJ, Johnson FB, Kirsi JJ, Robison RA, O'Neill KL.

Journal:

J Virol Methods (2008): 74

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	488 nm or 532 nm laser
Emission	575/26 nm filter
Instrument specification(s)	PE channel

Fluorescence microscope
Excitation	TRITC filter
Emission	TRITC filter
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	540 nm
Emission	570 nm
Cutoff	550 nm
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode

Documents

Protocol
Safety Data Sheet
Certificate of Origin
Certificate of Analysis
Brochure: Reactive Oxygen Species (ROS) Detection
Flyer: Reactive Oxygen Species (ROS)

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 28, 2025

Component A: ROS Brite™ 570	1 vial
Component B: Assay Buffer	1 bottle (20 mL)
Component C: DMSO	1 vial (100 µL)